国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2014年
9期
816-820
,共5页
钠钾氯联合转运蛋白1%钾氯联合转运蛋白2%术后疼痛%痛觉过敏
鈉鉀氯聯閤轉運蛋白1%鉀氯聯閤轉運蛋白2%術後疼痛%痛覺過敏
납갑록연합전운단백1%갑록연합전운단백2%술후동통%통각과민
Sodium-potassium-chloride co-transporter 1%Potassium-chloride co-transporter 2%Postoperative pain%Hyperalgesia
目的 检测大鼠切开模型脊髓背角及背根节(dorsal root ganglion,DRG)的钠钾氯联合转运蛋白1(sodiumpotassium-chloride co-transporter 1,NKCC1)与钾氯联合转运蛋白2(potassium-chloride co-transporter 2,KCC2)的表达. 方法 30只SD大鼠用随机数字表法随机分成对照组和切开后2h、2d、3d、7d组(每组6只),在吸入麻醉下制作切开模型或仅接受皮肤消毒.在相应时间点,在深麻醉下灌注、固定,取出L4～L5节段脊髓和双侧L5 DRG,用免疫组化方法测定NKCC1和KCC2蛋白表达. 结果 在正常大鼠,NKCC1在DRG表达,NKCC1及KCC2均在背角表达但以KCC2为主.切开后同侧DRG的NKCC1表达增加(P=0.010),与对照组阳性细胞数(18.0±2.8)比较,2 h(40.7±2.0)、2 d(54.7±9.8)、3 d(45.3±8.6)组的阳性细胞数显著增加(P=0.026,0.001,0.008);同侧脊髓背角NKCC1表达增加(P＜0.001),与对照组(14.33±0.56)比较,2 h(31.00±1.32)、2d(50.33±1.80)、3 d(38.67±3.04)、7 d(32.33±0.21)组的阳性细胞数显著增加(均为P＜0.01);同侧脊髓背角KCC2表达减少(P＜0.01),与对照组(42.7±2.6)比较,2 h(18.0±3.5)、2 d(18.0±1.7)、3 d(23.3±1.5)、7 d(24.7±1.1)组的阳性细胞数显著减少(均为P＜0.01). 结论 在大鼠切开模型,NKCC1在DRG及脊髓背角表达增加,KCC2在脊髓背角表达降低,提示NKCC1和KCC2参与了术后痛觉过敏形成机制,有希望成为术后疼痛治疗的新靶点.
目的 檢測大鼠切開模型脊髓揹角及揹根節(dorsal root ganglion,DRG)的鈉鉀氯聯閤轉運蛋白1(sodiumpotassium-chloride co-transporter 1,NKCC1)與鉀氯聯閤轉運蛋白2(potassium-chloride co-transporter 2,KCC2)的錶達. 方法 30隻SD大鼠用隨機數字錶法隨機分成對照組和切開後2h、2d、3d、7d組(每組6隻),在吸入痳醉下製作切開模型或僅接受皮膚消毒.在相應時間點,在深痳醉下灌註、固定,取齣L4～L5節段脊髓和雙側L5 DRG,用免疫組化方法測定NKCC1和KCC2蛋白錶達. 結果 在正常大鼠,NKCC1在DRG錶達,NKCC1及KCC2均在揹角錶達但以KCC2為主.切開後同側DRG的NKCC1錶達增加(P=0.010),與對照組暘性細胞數(18.0±2.8)比較,2 h(40.7±2.0)、2 d(54.7±9.8)、3 d(45.3±8.6)組的暘性細胞數顯著增加(P=0.026,0.001,0.008);同側脊髓揹角NKCC1錶達增加(P＜0.001),與對照組(14.33±0.56)比較,2 h(31.00±1.32)、2d(50.33±1.80)、3 d(38.67±3.04)、7 d(32.33±0.21)組的暘性細胞數顯著增加(均為P＜0.01);同側脊髓揹角KCC2錶達減少(P＜0.01),與對照組(42.7±2.6)比較,2 h(18.0±3.5)、2 d(18.0±1.7)、3 d(23.3±1.5)、7 d(24.7±1.1)組的暘性細胞數顯著減少(均為P＜0.01). 結論 在大鼠切開模型,NKCC1在DRG及脊髓揹角錶達增加,KCC2在脊髓揹角錶達降低,提示NKCC1和KCC2參與瞭術後痛覺過敏形成機製,有希望成為術後疼痛治療的新靶點.
목적 검측대서절개모형척수배각급배근절(dorsal root ganglion,DRG)적납갑록연합전운단백1(sodiumpotassium-chloride co-transporter 1,NKCC1)여갑록연합전운단백2(potassium-chloride co-transporter 2,KCC2)적표체. 방법 30지SD대서용수궤수자표법수궤분성대조조화절개후2h、2d、3d、7d조(매조6지),재흡입마취하제작절개모형혹부접수피부소독.재상응시간점,재심마취하관주、고정,취출L4～L5절단척수화쌍측L5 DRG,용면역조화방법측정NKCC1화KCC2단백표체. 결과 재정상대서,NKCC1재DRG표체,NKCC1급KCC2균재배각표체단이KCC2위주.절개후동측DRG적NKCC1표체증가(P=0.010),여대조조양성세포수(18.0±2.8)비교,2 h(40.7±2.0)、2 d(54.7±9.8)、3 d(45.3±8.6)조적양성세포수현저증가(P=0.026,0.001,0.008);동측척수배각NKCC1표체증가(P＜0.001),여대조조(14.33±0.56)비교,2 h(31.00±1.32)、2d(50.33±1.80)、3 d(38.67±3.04)、7 d(32.33±0.21)조적양성세포수현저증가(균위P＜0.01);동측척수배각KCC2표체감소(P＜0.01),여대조조(42.7±2.6)비교,2 h(18.0±3.5)、2 d(18.0±1.7)、3 d(23.3±1.5)、7 d(24.7±1.1)조적양성세포수현저감소(균위P＜0.01). 결론 재대서절개모형,NKCC1재DRG급척수배각표체증가,KCC2재척수배각표체강저,제시NKCC1화KCC2삼여료술후통각과민형성궤제,유희망성위술후동통치료적신파점.
Objective To investigate the expression of sodium-potassium-chloride co-transporter 1 (NKCC 1) and potassiumchloride co-transporter 2 (KCC2) in the spinal dorsal horn and dorsal root ganglion (DRG) of the incisional rat.Methods Adult male Sprague-Dawley rats were randomly divided into 5 groups of 6,a control and 4 incision groups at 2 h,2 d,3 d,7 d following incision.Incision was made or only skin degerming under inhalation anesthesia.At the specific time,rats were perfused with fixative under anesthesia,then the L4-L5 spinal cord and bilateral L5 DRG removed,and NKCC1 and KCC2 expression estimated by immunohistochemistry.Results In the intact rats,NKCC1 was expressed in the DRG,and both NKCC1 and KCC2 was expressed in the dorsal horn with KCC2 predominated.NKCC 1 increased in the DRG (P=0.010) following incision.Compared to controls(18.0±2.8),the number of NKCC1-positive neurons was significantly higher in ipsilateral DRG on 2 h (40.7±2.0),2 d (54.7±9.8),3 d (45.3±8.6) following incision (P=0.026,0.001,0.008).NKCC1 also increased in the ipsilateral dorsal horn (P＜0.01),compared tocontrols (14.33±0.56),the number of NKCC1-positive neurons was significantly higher on 2 h (31.00±1.32),2 d (50.33± 1.80),3 d(38.67±3.04),7 d (32.33±0.21) and all P＜0.01.KCC2 decreased in the dorsal horn following incision (P<0.01).Compared to controls(42.7±2.6),the number of KCC2-positive neurons was significantly lower in the dorsal horn on 2 h(18.0±3.5),2 d(18.0±1.7),3 d(23.3±1.5) and 7 d (24.7±1.1) following incision (all P＜0.01).Conclusions NKCC1 increased in the DRG and dorsal horn while KCC2 decreased in the dorsal horn in the incision model of rat,which suggested that NKCC1 and KCC2 participated in the mechanism of hyperalgesia following incision.
