国际麻醉学与复苏杂志
國際痳醉學與複囌雜誌
국제마취학여복소잡지
INTERNATIONAL JOURNAL OF ANESTHESIOLOGY AND RESUSCITATION
2014年
11期
981-984,1040
,共5页
异氟醚%缺血预处理%再灌注损伤%肾%脂肪乳剂
異氟醚%缺血預處理%再灌註損傷%腎%脂肪乳劑
이불미%결혈예처리%재관주손상%신%지방유제
Isoflurane%Ischemic preconditioning%Reperfusion injury%Kidney%Fat emulsions
目的 观察8％乳化异氟醚(emulsified isoflurane,EI)预处理对缺血/再灌注损伤(ischemia/reperfusion injury,I/RI)大鼠肾脏氧化应激反应的影响.方法 健康雄性Sprague-Dawley(SD)大鼠32只,右肾切除后采用随机数字表法分为4组(每组8只):假手术组(Sham组,仅分离左侧肾蒂,不阻断肾血流)、肾脏I/RI组(夹闭左侧肾蒂45 min后恢复再灌注)、EI预处理组(静脉泵注8％ EI 4 ml·kg-1h-130 min,停药15 min后夹闭左侧肾蒂,余同I/RI组)、脂肪方乳剂(lipid emulsion,LE)预处理组(静脉泵注30％ LE 4 ml· kg-1·h-1 30 min,停药15 min后夹闭左侧肾蒂,余同I/RI组).于肾脏再灌注3h时采集腹主动脉血测血清肌酐(creatinine,Cr)、尿素氮(blood urea nitrogen,BUN);取肾组织测超氧化物歧化酶活性及丙二醛(malondialdehyde,MDA)含量,光镜下观察肾脏病理学变化,并行肾小管Paller评分.采用黄嘌呤氧化酶法测定肾组织总超氧化物歧化酶活性,采用硫代巴比妥酸法测定肾组织MDA含量.结果 各组大鼠血清Cr、BUN、肾组织MDA含量及肾小管Paller评分:I/RI组[Cr(98±7) μmol/L,BUN(14.5±4.7) mmol/L,MDA(2.05±0.31) μmol/g,Paller(33.5±5.6)分]、EI组[Cr(62±6) μmol/L,BUN(9.3±2.3) mmol/L,MDA(1.61 ±0.28) μmol/g,Paller(18.5±4.5)分]、LE组[Cr(94±15) μmol/L,BUN(13.9±4.5) mmol/L,MDA(1.91±0.35) μmol/g,Paller(32.2±3.8)分]与Sham组[Cr(24±6)μmol/L,BUN(6.5±1.6) mmol/L,MDA(1.32±0.19) μmol/g,Paller(1.6±0.9)分]比较,显著升高(P＜0.05);肾组织超氧化物歧化酶活性:I/RI组[(78±12)×103 U/g]、EI组[(97±7)×103 U/g]、LE组[(79±13)×103 U/g]与Sham组[(117±10)×103 U/g]比较,显著降低(P＜0.05).分别与I/RI组和LE组比较,EI组血清Cr、BUN、肾组织MDA含量及肾小管Paller评分均降低(P＜0.05),肾组织超氧化物歧化酶活性均升高(P＜0.05).I/RI组和LE组上述各指标比较差异无统计学意义(P＞0.05).I/RI组和LE组肾组织病理学损伤最严重,EI组肾组织病理学损伤明显减轻.结论 8％EI预处理可通过抑制肾组织氧化应激反应减轻大鼠肾脏I/RI.
目的 觀察8％乳化異氟醚(emulsified isoflurane,EI)預處理對缺血/再灌註損傷(ischemia/reperfusion injury,I/RI)大鼠腎髒氧化應激反應的影響.方法 健康雄性Sprague-Dawley(SD)大鼠32隻,右腎切除後採用隨機數字錶法分為4組(每組8隻):假手術組(Sham組,僅分離左側腎蒂,不阻斷腎血流)、腎髒I/RI組(夾閉左側腎蒂45 min後恢複再灌註)、EI預處理組(靜脈泵註8％ EI 4 ml·kg-1h-130 min,停藥15 min後夾閉左側腎蒂,餘同I/RI組)、脂肪方乳劑(lipid emulsion,LE)預處理組(靜脈泵註30％ LE 4 ml· kg-1·h-1 30 min,停藥15 min後夾閉左側腎蒂,餘同I/RI組).于腎髒再灌註3h時採集腹主動脈血測血清肌酐(creatinine,Cr)、尿素氮(blood urea nitrogen,BUN);取腎組織測超氧化物歧化酶活性及丙二醛(malondialdehyde,MDA)含量,光鏡下觀察腎髒病理學變化,併行腎小管Paller評分.採用黃嘌呤氧化酶法測定腎組織總超氧化物歧化酶活性,採用硫代巴比妥痠法測定腎組織MDA含量.結果 各組大鼠血清Cr、BUN、腎組織MDA含量及腎小管Paller評分:I/RI組[Cr(98±7) μmol/L,BUN(14.5±4.7) mmol/L,MDA(2.05±0.31) μmol/g,Paller(33.5±5.6)分]、EI組[Cr(62±6) μmol/L,BUN(9.3±2.3) mmol/L,MDA(1.61 ±0.28) μmol/g,Paller(18.5±4.5)分]、LE組[Cr(94±15) μmol/L,BUN(13.9±4.5) mmol/L,MDA(1.91±0.35) μmol/g,Paller(32.2±3.8)分]與Sham組[Cr(24±6)μmol/L,BUN(6.5±1.6) mmol/L,MDA(1.32±0.19) μmol/g,Paller(1.6±0.9)分]比較,顯著升高(P＜0.05);腎組織超氧化物歧化酶活性:I/RI組[(78±12)×103 U/g]、EI組[(97±7)×103 U/g]、LE組[(79±13)×103 U/g]與Sham組[(117±10)×103 U/g]比較,顯著降低(P＜0.05).分彆與I/RI組和LE組比較,EI組血清Cr、BUN、腎組織MDA含量及腎小管Paller評分均降低(P＜0.05),腎組織超氧化物歧化酶活性均升高(P＜0.05).I/RI組和LE組上述各指標比較差異無統計學意義(P＞0.05).I/RI組和LE組腎組織病理學損傷最嚴重,EI組腎組織病理學損傷明顯減輕.結論 8％EI預處理可通過抑製腎組織氧化應激反應減輕大鼠腎髒I/RI.
목적 관찰8％유화이불미(emulsified isoflurane,EI)예처리대결혈/재관주손상(ischemia/reperfusion injury,I/RI)대서신장양화응격반응적영향.방법 건강웅성Sprague-Dawley(SD)대서32지,우신절제후채용수궤수자표법분위4조(매조8지):가수술조(Sham조,부분리좌측신체,불조단신혈류)、신장I/RI조(협폐좌측신체45 min후회복재관주)、EI예처리조(정맥빙주8％ EI 4 ml·kg-1h-130 min,정약15 min후협폐좌측신체,여동I/RI조)、지방방유제(lipid emulsion,LE)예처리조(정맥빙주30％ LE 4 ml· kg-1·h-1 30 min,정약15 min후협폐좌측신체,여동I/RI조).우신장재관주3h시채집복주동맥혈측혈청기항(creatinine,Cr)、뇨소담(blood urea nitrogen,BUN);취신조직측초양화물기화매활성급병이철(malondialdehyde,MDA)함량,광경하관찰신장병이학변화,병행신소관Paller평분.채용황표령양화매법측정신조직총초양화물기화매활성,채용류대파비타산법측정신조직MDA함량.결과 각조대서혈청Cr、BUN、신조직MDA함량급신소관Paller평분:I/RI조[Cr(98±7) μmol/L,BUN(14.5±4.7) mmol/L,MDA(2.05±0.31) μmol/g,Paller(33.5±5.6)분]、EI조[Cr(62±6) μmol/L,BUN(9.3±2.3) mmol/L,MDA(1.61 ±0.28) μmol/g,Paller(18.5±4.5)분]、LE조[Cr(94±15) μmol/L,BUN(13.9±4.5) mmol/L,MDA(1.91±0.35) μmol/g,Paller(32.2±3.8)분]여Sham조[Cr(24±6)μmol/L,BUN(6.5±1.6) mmol/L,MDA(1.32±0.19) μmol/g,Paller(1.6±0.9)분]비교,현저승고(P＜0.05);신조직초양화물기화매활성:I/RI조[(78±12)×103 U/g]、EI조[(97±7)×103 U/g]、LE조[(79±13)×103 U/g]여Sham조[(117±10)×103 U/g]비교,현저강저(P＜0.05).분별여I/RI조화LE조비교,EI조혈청Cr、BUN、신조직MDA함량급신소관Paller평분균강저(P＜0.05),신조직초양화물기화매활성균승고(P＜0.05).I/RI조화LE조상술각지표비교차이무통계학의의(P＞0.05).I/RI조화LE조신조직병이학손상최엄중,EI조신조직병이학손상명현감경.결론 8％EI예처리가통과억제신조직양화응격반응감경대서신장I/RI.
Objective To observe the effect of 8％ emulsified isoflurane (EI) preconditioning on oxidative stress by kidney ischemia repeffusion injury(I/RI) in rats.Methods Thirty-two healthy male Sprague-Dawley(SD) rats were randomly divided into 4 groups after right nephrectomy:① sham operation (Sham) group:the left renal pedicle was not clamped.② I/RI group:kidney I/RI was induced by occlusion of left renal pedicle for 45 min with atraumatic microclips followed by 3 h reperfusion.③ EI preconditioning group and ④ lipid emulsion (LE) preconditioning group:8％ EI or 30％ LE 4 ml·kg-1·h-1 was infused intravenously for 30 min,respectively,15 min before kidney I/RI was induced.At 3 h of reperfusion,the blood samples from abdominal aorta were taken for assessment of creatinine (Cr) and blood urea nitrogen (BUN).The rats were then sacrificed and the left kidney was removed.Renal superoxide dismutase (SOD) activity and malondialdehyde (MDA) content were detected.The renal tissues were stained with hematoxylin-eosin for optical microscopic examination and assessment of necrotic injury of the proximal tubules.Results Compared with Sham group [Cr(24±6) μmol/L,BUN(6.5±1.6) mmol/L,SOD(117±10)×103 U/g,MDA(1.32±-0.19) μmol/g,Paller scores(1.6± 0.9)],serum Cr and BUN levels,and renal MDA content and Paller scores were obviously higher,and renal SOD activity was lower in I/RI,EI and LE group (P＜0.05)[Cr:I/RI group (98±7) μmol/L,EI group (62±6) μmol/L,LE group (94±15) μmol/L.BUN:I/RI group(14.5±4.7) mmol/L,EI(9.3±2.3) mmol/L,LE group(13.9±4.5) mmol/L.SOD:I/RI group(78±12)×103 U/g,EI group(97±7)×103 U/g,LE group(79±13)×103 U/g,MDA:I/RI group(2.05±0.31) μmol/g,EI group(1.61±0.28) μ mol/g,LE group (1.91 ±0.35) μmol/g,Paller scores:I/RI group (33.5±5.6),EI group (18.5±4.5),LE group (32.2±3.8)].The serum Cr and BUN levels,and renal MDA content and Paller scores were significantly decreased,while renal SOD activity was significantly increased in EI group compared with I/RI and LE group,respectively (P＜0.05).There was no significant difference in the above parameters between I/RI and LE group (P＞0.05).The renal pathological damage was most serious in I/RI and LE group,but was significantly attenuated in LE group.Conclusions Preconditioning with 8％ emulsified isoflurane can attenuate kidney I/RI by inhibiting oxidative stress response in rats.
