国际内分泌代谢杂志
國際內分泌代謝雜誌
국제내분비대사잡지
INTERNATIONAL JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2013年
5期
298-301
,共4页
戚筠%尚桂莲%邹润梅%徐焱成
慼筠%尚桂蓮%鄒潤梅%徐焱成
척균%상계련%추윤매%서염성
枸杞多糖%MIN6细胞%细胞增殖%细胞凋亡
枸杞多糖%MIN6細胞%細胞增殖%細胞凋亡
구기다당%MIN6세포%세포증식%세포조망
Lycium barbarum polysaccharide%MIN6 cells%Cells viability%Apoptosis
目的 研究不同浓度枸杞多糖对小鼠胰岛β细胞系MIN6细胞增殖活性和凋亡的影响.方法 培养MIN6细胞,分为对照组、不同浓度枸杞多糖干预组,分别给予0,100,200,400 mg/L枸杞多糖干预24 h.噻唑蓝(MTT)法检测MIN6细胞增殖活性,流式细胞术检测MIN6细胞凋亡率,Western印迹分析磷酸化细胞外信号调节激酶(ERK)及B淋巴细胞瘤-2(Bcl-2)蛋白表达.结果 与对照组相比,100、200 mg/L枸杞多糖干预组MIN6细胞增殖显著增加(P均＜0.01),MIN6细胞凋亡显著受抑制(P均＜0.01),而400 mg/L枸杞多糖干预组细胞增殖活性降低(P＜0.05),MIN6细胞凋亡增加(P＜0.01).Western印迹显示,与对照组相比,100,200,400 mg/L枸杞多糖干预组Bcl-2表达(F=65.26,P＜0.01)及磷酸化ERK水平(F=14.85,P＜0.01)均增加,而400 mg/L枸杞多糖干预组Bcl-2与磷酸化ERK表达下降(P均＜0.05),200 mg/L枸杞多糖干预组与400 mg/L枸杞多糖干预组相比,Bcl-2与磷酸化ERK水平差异均有统计学意义(P均＜0.01).结论 低浓度枸杞多糖可促进MIN6细胞增殖,减少MIN6细胞凋亡,而高浓度枸杞多糖作用相反,其机制可能与ERK信号通路有关.
目的 研究不同濃度枸杞多糖對小鼠胰島β細胞繫MIN6細胞增殖活性和凋亡的影響.方法 培養MIN6細胞,分為對照組、不同濃度枸杞多糖榦預組,分彆給予0,100,200,400 mg/L枸杞多糖榦預24 h.噻唑藍(MTT)法檢測MIN6細胞增殖活性,流式細胞術檢測MIN6細胞凋亡率,Western印跡分析燐痠化細胞外信號調節激酶(ERK)及B淋巴細胞瘤-2(Bcl-2)蛋白錶達.結果 與對照組相比,100、200 mg/L枸杞多糖榦預組MIN6細胞增殖顯著增加(P均＜0.01),MIN6細胞凋亡顯著受抑製(P均＜0.01),而400 mg/L枸杞多糖榦預組細胞增殖活性降低(P＜0.05),MIN6細胞凋亡增加(P＜0.01).Western印跡顯示,與對照組相比,100,200,400 mg/L枸杞多糖榦預組Bcl-2錶達(F=65.26,P＜0.01)及燐痠化ERK水平(F=14.85,P＜0.01)均增加,而400 mg/L枸杞多糖榦預組Bcl-2與燐痠化ERK錶達下降(P均＜0.05),200 mg/L枸杞多糖榦預組與400 mg/L枸杞多糖榦預組相比,Bcl-2與燐痠化ERK水平差異均有統計學意義(P均＜0.01).結論 低濃度枸杞多糖可促進MIN6細胞增殖,減少MIN6細胞凋亡,而高濃度枸杞多糖作用相反,其機製可能與ERK信號通路有關.
목적 연구불동농도구기다당대소서이도β세포계MIN6세포증식활성화조망적영향.방법 배양MIN6세포,분위대조조、불동농도구기다당간예조,분별급여0,100,200,400 mg/L구기다당간예24 h.새서람(MTT)법검측MIN6세포증식활성,류식세포술검측MIN6세포조망솔,Western인적분석린산화세포외신호조절격매(ERK)급B림파세포류-2(Bcl-2)단백표체.결과 여대조조상비,100、200 mg/L구기다당간예조MIN6세포증식현저증가(P균＜0.01),MIN6세포조망현저수억제(P균＜0.01),이400 mg/L구기다당간예조세포증식활성강저(P＜0.05),MIN6세포조망증가(P＜0.01).Western인적현시,여대조조상비,100,200,400 mg/L구기다당간예조Bcl-2표체(F=65.26,P＜0.01)급린산화ERK수평(F=14.85,P＜0.01)균증가,이400 mg/L구기다당간예조Bcl-2여린산화ERK표체하강(P균＜0.05),200 mg/L구기다당간예조여400 mg/L구기다당간예조상비,Bcl-2여린산화ERK수평차이균유통계학의의(P균＜0.01).결론 저농도구기다당가촉진MIN6세포증식,감소MIN6세포조망,이고농도구기다당작용상반,기궤제가능여ERK신호통로유관.
Objective To study the effects of different concentration lycium barbarum polysaccharide (LBP) on mouse islet β cell line MIN6 cells viability and apoptosis.Methods MIN6 cells were cultured and divided into control group and different concentration LBP intervene group,treated separately with 0,100,200,400 mg/L LBP for 24 h.Cells viability and apoptosis were separately detected by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and flow cytometry.The expression of phosphorylated extracellular regulated protein kinase (ERK) and B-cell lymphoma 2 (Bcl-2) were analyzed by Western blot.Results Compared with control group,MIN6 cells viability was promoted (all P ＜ 0.01),apoptosis was repressed (all P ＜0.01) in 100,200 mg/L LBP intervene group,while MIN6 cells viability was decreased (P ＜ 0.05) and apoptosis was increased (P ＜ 0.05) in 400 mg/L LBP intervene group.Compared with control group,protein levels of Bcl-2 and phosphorylated ERK were increased in 100,200,400 mg/L LBP intervene group (F =65.26,P ＜0.01 ; F =14.85,P ＜0.01),and decreased in 400 mg/L LBP intervene group.The difference of levels of Bcl-2 and phosphorylated ERK were statistically significant between 200 mg/L LBP intervene group and 400 mg/L LBP intervene group (all P ＜ 0.01).Conclusions Lower concentration LBP could promote the proliferation and inhibit the apoptosis of MIN6 cells,while the effects of higher concentration LBP were on the contrary.The mechanisms were associated with ERK signaling pathway.
