国际内分泌代谢杂志
國際內分泌代謝雜誌
국제내분비대사잡지
INTERNATIONAL JOURNAL OF ENDOCRINOLOGY AND METABOLISM
2014年
1期
15-17
,共3页
李新%孙家忠%孙苏欣%杨杪%吴玉文
李新%孫傢忠%孫囌訢%楊杪%吳玉文
리신%손가충%손소흔%양초%오옥문
CTRP3%3T3-L1脂肪细胞%脂联素%AMPK
CTRP3%3T3-L1脂肪細胞%脂聯素%AMPK
CTRP3%3T3-L1지방세포%지련소%AMPK
CTRP3%3T3-L1 adipocytes%Adiponectin%AMPK
目的 探讨AMP活化蛋白激酶(AMPK)信号通路在Clq/肿瘤坏死因子相关蛋白3(CTRP3)调节3T3-L1脂肪细胞脂联素表达中的作用.方法将3T3-L1脂肪细胞分为对照组、CTRP3(250μg/L)干预组及CTRP3(250 μg/L)+ Compound C(10 μmol/L预处理1h)干预组.以酶联免疫吸附法(ELISA)检测细胞培养上清中脂联素含量,实时定量PCR检测脂联素mRNA相对表达水平,Western印迹检测AMPK(thr172)蛋白相对表达量.结果 与对照组相比,CTRP3干预组脂联素mRNA表达及蛋白分泌分别增加53.0％(q =15.09,P＜0.01)及63.3％(q =8.11,P＜0.01),AMPK(thr172)表达增加43.0％(q =14.64,P＜0.01);与CTRP3干预组相比,CTRP3+Compound C干预组AMPK(thr172)蛋白的表达降低57.3％(q=27.92,P＜0.01),脂联素mRNA表达及蛋白分泌分别下降26.1％(q=11.39,P＜0.01)及54.1％(q =11.31,P＜0.01).结论 AMPK信号通路参与了CTRP3对3T3-L1脂肪细胞脂联素表达的调控过程.
目的 探討AMP活化蛋白激酶(AMPK)信號通路在Clq/腫瘤壞死因子相關蛋白3(CTRP3)調節3T3-L1脂肪細胞脂聯素錶達中的作用.方法將3T3-L1脂肪細胞分為對照組、CTRP3(250μg/L)榦預組及CTRP3(250 μg/L)+ Compound C(10 μmol/L預處理1h)榦預組.以酶聯免疫吸附法(ELISA)檢測細胞培養上清中脂聯素含量,實時定量PCR檢測脂聯素mRNA相對錶達水平,Western印跡檢測AMPK(thr172)蛋白相對錶達量.結果 與對照組相比,CTRP3榦預組脂聯素mRNA錶達及蛋白分泌分彆增加53.0％(q =15.09,P＜0.01)及63.3％(q =8.11,P＜0.01),AMPK(thr172)錶達增加43.0％(q =14.64,P＜0.01);與CTRP3榦預組相比,CTRP3+Compound C榦預組AMPK(thr172)蛋白的錶達降低57.3％(q=27.92,P＜0.01),脂聯素mRNA錶達及蛋白分泌分彆下降26.1％(q=11.39,P＜0.01)及54.1％(q =11.31,P＜0.01).結論 AMPK信號通路參與瞭CTRP3對3T3-L1脂肪細胞脂聯素錶達的調控過程.
목적 탐토AMP활화단백격매(AMPK)신호통로재Clq/종류배사인자상관단백3(CTRP3)조절3T3-L1지방세포지련소표체중적작용.방법장3T3-L1지방세포분위대조조、CTRP3(250μg/L)간예조급CTRP3(250 μg/L)+ Compound C(10 μmol/L예처리1h)간예조.이매련면역흡부법(ELISA)검측세포배양상청중지련소함량,실시정량PCR검측지련소mRNA상대표체수평,Western인적검측AMPK(thr172)단백상대표체량.결과 여대조조상비,CTRP3간예조지련소mRNA표체급단백분비분별증가53.0％(q =15.09,P＜0.01)급63.3％(q =8.11,P＜0.01),AMPK(thr172)표체증가43.0％(q =14.64,P＜0.01);여CTRP3간예조상비,CTRP3+Compound C간예조AMPK(thr172)단백적표체강저57.3％(q=27.92,P＜0.01),지련소mRNA표체급단백분비분별하강26.1％(q=11.39,P＜0.01)급54.1％(q =11.31,P＜0.01).결론 AMPK신호통로삼여료CTRP3대3T3-L1지방세포지련소표체적조공과정.
Objective To investigate the role of AMP activated protein kinase (AMPK)signal in the modulation of Clq/TNF related protein 3 (CTRP3) on the expression of adiponectin in adipocytes.Methods The 3T3-L1 adipocytes were divided into control group,CTRP3 (250 μg/L) intervention group and CTRP3 (250 μg/L)+ Compound C (10 μmol/L pretreated for 1 h) intervention group.The content of adiponectin in supernatant was detected by enzyme linked immunosorbent assay.The relative expression of adiponectin mRNA was detected by real-time polymerase chain reaction.The relative expression of AMPK (thr172) was detected by Western blot.Results Compared with control group,expression of adiponectin mRNA and protein increased 53.0％ (q =15.09,P＜0.01) and 63.3％ (q =8.11,P＜0.01) respectively,the expression of AMPK(thr172) increased 43.0％ (q =14.64,P＜0.01) in CTRP3 intervention group.Compared with CTRP3 intervention group,the expression of AMPK(thr172) in CTRP3+ Compound C intervention group decreased 57.3％ (q =27.92,P＜0.01),expression of adiponectin mRNA and protein decreased 26.1％ (q =11.39,P＜0.01) and 54.1％ (q =11.31,P＜0.01) respectively.Conclusion AMPK signal participates in the modulation of CTRP3 on the expression of adiponectin in 3T3-L1 adipocytes.
