CAJ | 학술논문

目的研究连续6d睡眠剥夺对Wistar大鼠甲状腺功能及抗甲状腺抗体的影响.方法健康3月龄Wistar大鼠30只,随机数字法分为睡眠剥夺组(SD组)、实验对照组(TC组)、空白对照组(CC组),每组雌鼠、雄鼠各5只,利用“小平台水环境法”建立大鼠睡眠剥夺模型,以大平台及正常笼养组分别作为实验对照和空白对照.将SD组和TC组大鼠置于24 h拟光照环境中,对SD组大鼠进行连续6d睡眠剥夺;CC组模拟正常作息时间,饲养在每天12h拟光照、12h黑暗的环境中.6d后股动脉放血处死所有大鼠,留取甲状腺组织及血液,采用化学发光免疫分析法检测不同组大鼠血清总T3(TT3)、总T4(TT4)、游离T3(FT3)、游离T4(FT4)、促甲状腺激素(TSH)水平,采用放射免疫分析法检测血清甲状腺过氧化物酶(TPO)抗体、甲状腺球蛋白(Tg)抗体和Tg浓度.结果 SD组大鼠TT3水平较TC组和CC组明显升高(P均＜0.05);TT4、FT4水平明显低于TC组和CC组(P均＜0.05);FT3、Tg水平略有升高;TSH、TPO抗体、Tg抗体水平较TC组和CC组差异无统计学意义.结论连续6d睡眠剥夺可影响大鼠甲状腺功能,但甲状腺功能尚可通过增加T4向T3转换而代偿.
목적 연구련속6d수면박탈대Wistar대서갑상선공능급항갑상선항체적영향.방법 건강3월령Wistar대서30지,수궤수자법분위수면박탈조(SD조)、실험대조조(TC조)、공백대조조(CC조),매조자서、웅서각5지,이용“소평태수배경법”건립대서수면박탈모형,이대평태급정상롱양조분별작위실험대조화공백대조.장SD조화TC조대서치우24 h의광조배경중,대SD조대서진행련속6d수면박탈;CC조모의정상작식시간,사양재매천12h의광조、12h흑암적배경중.6d후고동맥방혈처사소유대서,류취갑상선조직급혈액,채용화학발광면역분석법검측불동조대서혈청총T3(TT3)、총T4(TT4)、유리T3(FT3)、유리T4(FT4)、촉갑상선격소(TSH)수평,채용방사면역분석법검측혈청갑상선과양화물매(TPO)항체、갑상선구단백(Tg)항체화Tg농도.결과 SD조대서TT3수평교TC조화CC조명현승고(P균＜0.05);TT4、FT4수평명현저우TC조화CC조(P균＜0.05);FT3、Tg수평략유승고;TSH、TPO항체、Tg항체수평교TC조화CC조차이무통계학의의.결론 련속6d수면박탈가영향대서갑상선공능,단갑상선공능상가통과증가T4향T3전환이대상.
Objective To study the effects of sleep deprivation for six-consecutive-day on thyroid function and anti-thyroid antibodies in Wistar rats.Methods Thirty rats aged 3 month healthy Wistar were randomly divided into three groups according to a random number table:sleep deprivation group(SD group),tank cage group (TC group) and control group (CC group),with five females and five males in each group.Sleep deprivation was induced in rats by housing them on small platforms over water.Controls were housed either in tanks with large platforms(TC group) or in normal cage(CC group).Rats in SD group and TC group were placed in 24-hour proposed lighting environment,and rats in SD group were deprived of sleep for 6 consecutive days.Rats in CC group were placed in 12 hours light and 12 hours dark environment.Six days later,all rats were sacrificed,thyroid tissue and blood from femoral artery were saved.Total T3(TT3),totalT4(T4),free T3 (FT3),free T4 (FT4) and thyroid stimulating hormone (TSH) in serum were detected by chemiluminescence immunoassay,and the level of anti-thyroid peroxidase(TPOAb),anti-thyroglobulin (TgAb) and thyroglobulin (Tg) were evaluated by radioimmunoassay.Results TT3 in SD group were significandy higher than that of rats in TC and CC group (all P ＜0.05).Compared with TC and CC group,the concentrations of TT4,FT4 were lower (all P ＜ 0.05),and FT3 and Tg concentrations were a little higher.No significant changes were found in TSH,TPOAb and TgAb among the three groups.Conclusion Six consecutive days of sleep deprivation can influence thyroid function,but can be compensated by transferring more T4 to T3.