CAJ | 학술논문

目的通过测定妊娠糖尿病(GDM)及正常妊娠妇女血清visfatin、胎盘组织visfatin及过氧化物酶体增殖物活化受体(PPAR)γ的表达,探讨visfatin、PPARγ在GDM中的作用及相互关系.方法选取2011年10月至2012年10月在河北省人民医院妇产科行择期剖宫产终止妊娠的患者57例,其中GDM组30例、正常妊娠组27名.酶联免疫吸附法测定血清visfatin水平,实时荧光定量反转录PCR测定胎盘组织visfatin及PPARγ mRNA表达水平,免疫蛋白印迹法检测胎盘组织visfatin及PPARγ蛋白的表达水平.结果 GDM组产前血清visfatin水平明显低于正常妊娠组[(6.72±3.79)ng/ml vs.(8.87±4.08) ng/ml,t=-2.06,P=0.04];胎盘组织visfatin mRNA及蛋白表达水平在两组间无明显差异(P＞0.05);GDM组PPARγ mRNA及蛋白表达水平低于正常妊娠组[(0.26±0.11)vs.(0.60±0.41),t=-2.77,P＜0.05;(0.34 ±0.09)vs.(0.73±0.13),t=-7.03,P＜0.05],在GDM组,胎盘组织visfatin mRNA表达与PPARγ mRNA表达水平呈正相关(r=0.67,P=0.02).结论 Visfatin在GDM的发病中起一定作用,并与胎盘组织中PPARγ mRNA表达相关.
목적 통과측정임신당뇨병(GDM)급정상임신부녀혈청visfatin、태반조직visfatin급과양화물매체증식물활화수체(PPAR)γ적표체,탐토visfatin、PPARγ재GDM중적작용급상호관계.방법 선취2011년10월지2012년10월재하북성인민의원부산과행택기부궁산종지임신적환자57례,기중GDM조30례、정상임신조27명.매련면역흡부법측정혈청visfatin수평,실시형광정량반전록PCR측정태반조직visfatin급PPARγ mRNA표체수평,면역단백인적법검측태반조직visfatin급PPARγ단백적표체수평.결과 GDM조산전혈청visfatin수평명현저우정상임신조[(6.72±3.79)ng/ml vs.(8.87±4.08) ng/ml,t=-2.06,P=0.04];태반조직visfatin mRNA급단백표체수평재량조간무명현차이(P＞0.05);GDM조PPARγ mRNA급단백표체수평저우정상임신조[(0.26±0.11)vs.(0.60±0.41),t=-2.77,P＜0.05;(0.34 ±0.09)vs.(0.73±0.13),t=-7.03,P＜0.05],재GDM조,태반조직visfatin mRNA표체여PPARγ mRNA표체수평정정상관(r=0.67,P=0.02).결론 Visfatin재GDM적발병중기일정작용,병여태반조직중PPARγ mRNA표체상관.
Objective To investigate the serum visfatin and placental level of visfatin,peroxisome proliferator-activated receptor (PPAR) γ in pregnant women with or without gestational diabetes mellitus (GDM),analyze their co-relationship and roles in GDM.Methods Fifty-seven pregnant women were enrolled at the department of Obstetrics and Gynecology in Hebei general hospital from October 2011 to October 2012.All pregnancies were undertaken elective C-section.Thirty of these women suffered from GDM.The remaining 27 age-matched pregnant women with normal oral glucose tolerance test were served as controls.Serum visfatin level,its mRNA expression,PPARγ mRNA expression (quantitative real-time PCR) and its protein level in placental tissue from women with GDM and normal pregnant controls were measured.Results Serum visfatin was significantly lower in women with GDM compared with those in healthy controls [(6.72 ± 3.79) ng/ml vs.(8.87 ± 4.08) ng/ml,t =-2.06,P =0.04].However,there were no differences in visfatin mRNA and protein expression between the two groups(P ＞ 0.05).PPARγ mRNA and protein expression were lower in GDM group compared with the healthy contol group [(0.26 ± 0.11) vs.(0.60 ± 0.41),t=-2.77,P＜0.05;(0.34 ±0.09) vs.(0.73 ±0.13),t =-7.03,P ＜0.05].The visfatin mRNA expression in placenta was positively correlated with PPARγ mRNA expression in GDM group (r =0.67,P =0.02).Conclusions Visfatin may play a role in the pathogenesis of GDM,and there is an association between visfatin and PPARγ in GDM.