CAJ | 학술논문

目的探讨急性脑血管病患者血浆可溶性血栓调节蛋白(soluble thrombomodulin,sTM)含量的变化和血栓调节蛋白基因Ala455Val(C1418T)多态性的分布.方法在79例汉族急性脑血管病患者(脑出血患者30例,脑梗死患者49例)和30名年龄和性别相匹配的健康对照者中,采用聚合酶链反应限制性酶切片段长度多态性分析技术检测TM Ala455Val(C1418T)多态性,酶联免疫吸附试验检测血浆sTM含量.结果脑梗死组和脑出血组sIM含量分别为(32.08±6.98)ng/ml和(38.93±6.77)ng/ml,显著高于正常对照组的(9.90±3.09)ng/ml(P均<0.01),而且与高血压、糖尿病、心脏病等高危因素无关;脑出血组和脑梗死组TM 1418C/C基因型频率分别为53.3%和57.1%,高于对照组的40%,但无统计学差异.结论无论足脑出血还是腑梗死患者,血浆sTM含量均显著高于正常人群;汉族人群中脑出血和脑梗死患者TM 1418C/C基因型频率虽然高于正常人群,但并非脑血管病的易感基因.
목적 탐토급성뇌혈관병환자혈장가용성혈전조절단백(soluble thrombomodulin,sTM)함량적변화화혈전조절단백기인Ala455Val(C1418T)다태성적분포.방법 재79례한족급성뇌혈관병환자(뇌출혈환자30례,뇌경사환자49례)화30명년령화성별상필배적건강대조자중,채용취합매련반응한제성매절편단장도다태성분석기술검측TM Ala455Val(C1418T)다태성,매련면역흡부시험검측혈장sTM함량.결과 뇌경사조화뇌출혈조sIM함량분별위(32.08±6.98)ng/ml화(38.93±6.77)ng/ml,현저고우정상대조조적(9.90±3.09)ng/ml(P균<0.01),이차여고혈압、당뇨병、심장병등고위인소무관;뇌출혈조화뇌경사조TM 1418C/C기인형빈솔분별위53.3%화57.1%,고우대조조적40%,단무통계학차이.결론 무론족뇌출혈환시부경사환자,혈장sTM함량균현저고우정상인군;한족인군중뇌출혈화뇌경사환자TM 1418C/C기인형빈솔수연고우정상인군,단병비뇌혈관병적역감기인.
Objective To investigate the changes of plasma soluble thrombomodulin (sTM) and the distribution of thrombomodulin Ala455Val (C1418T) polymorphism in patients with acute cerebrovascular diseases. Methods Among the 79 Chinese Han patients with acute cerebrovascular disease (30 intracerebral hemorrhage, and 49 cerebral infarction) and 30 age-and sex-matched healthy controls, polymerase chain reaction-restriction fragment length polymor phism (PCR-RFLP) analysis was used to detect TM Ala455Val (C1418T) polymorphism, and enzyme-linked immunosorbent assay (ELISA) was used to detect sTM level. Results The sTM levels in the cerebral infarction and intracerebral hemorrhage groups were 32. 08 ± 6. 98 ng/ml and 38. 93 ±6. 77 ng/ml, respectively. They were significantly higher than 9. 90 ±3. 09 ng/mL in the healthy control group (all P < 0. 01) and were not associated with the high risk factors, such as hypertension, diabetes, and cardiac disease etc; TM 1418C/C genotype frequencies in the intthe intracerebral hemorrhage and cerebral infarction groups were 53. 3% and 57.1% respectively, and they were higher than 40% of the control group, but there was no statistically significant differences. Conclusions No matter the patients with intracerebral hemorrhage or cerebral infarction, the plasma sTM levels were significantly higher than the normal population; although TM 1418C/C genotype frequencies in patients with intracerebral hemorrhage and cerebral infarction in Chinese Han population were higher than normal population, they were not the susceptible genes of cerebrovascular disease.