国际脑血管病杂志
國際腦血管病雜誌
국제뇌혈관병잡지
INTERNATIONAL JOURNAL OF CEREBROVASCULAR DISEASES
2012年
10期
739-744
,共6页
姜雪松%高美玲%马爱军%王琨%王源%范凌燕%韩莹%伊朋%李翠玲%潘旭东
薑雪鬆%高美玲%馬愛軍%王琨%王源%範凌燕%韓瑩%伊朋%李翠玲%潘旭東
강설송%고미령%마애군%왕곤%왕원%범릉연%한형%이붕%리취령%반욱동
基质金属蛋白酶3%多态现象,遗传学%卒中%脑缺血
基質金屬蛋白酶3%多態現象,遺傳學%卒中%腦缺血
기질금속단백매3%다태현상,유전학%졸중%뇌결혈
Matrix Metalloproteinase 3%Polymorphism,Genetic%Stroke%Brain Ischemia
目的 探讨基质金属蛋白酶-3(matrix metalloprotemase-3,MMP-3)血浆水平及其基因Lys45Glu(rs679620)多态性与缺血性卒中及其TOAST亚型的关系.方法 纳入根据TOAST病因学分型分为大动脉粥样硬化性卒中(large artery atherosclerotic stroke,LAA)和小动脉闭塞性卒中(small artery occlusion stroke,SAO)患者(缺血性卒中组)和健康体检者(对照组).采用酶联免疫吸附法检测血浆MMP-3水平,多聚酶链反应-限制性片段长度多态性法检测MMP-3 Lys45 Glu基因型.结果共纳入急性缺血性卒中患者233例,其中LAA 162例,SAO 71例;200名健康体检者作为对照组.缺血性卒中组血浆MMP-3水平显著高于对照组[(253.99±75.02)ng/ml对(196.38±78.17) ng/ml;=7.813,P=0.000];LAA组[(262.81±69.23)ng/ml]血浆MMP-3水平均显著高于SAO组[(233.85±83.90)ng/ml,P=0.008]和对照组(P=0.000),SAO组也显著高于对照组(P=0.000).多变量logistic回归分析显示,血清MMP-3水平增高是缺血性卒中的独立危险因素[优势比(odds ratio,OR)1.012,95％可信区间(confidence interval,CI)1.008～1.015;P=0.000].缺血性卒中组MMP-3Lys45Glu基因型(x2 =2.085,P=0.353)和等位基因(x2 =2.29,P=0.130)频率与对照组均无显著差异.LAA、SAO和对照组之间MMP-3 Lys45Glu基因型频率存在显著差异(x2=10.39,P=0.034),其中LAA组AA +GA频率显著高于SAO组(65.4％对49.3％;x2=5.375,P=0.020)和对照组(65.4％对54.0％;x2 =4.84,P=0.028);3组间MMP-3 Lys45Glu等位基因频率无显著性差异(x2=3.887,P=0.143).多变量logistic回归分析显示,Lys45Glu多态性是LAA的独立危险因素(OR1.783,95％ CI1.183 ～2.688;P=0.006).AA基因型(n=73)、GA基因型(n=176)和GG基因型(n=184)患者血浆MMP-3水平分别为(235.70±70.85)ng/ml、(244.20±85.90) ng/ml和(207.98±77.61) ng/ml,3组间存在显著性差异(F=9.682,P=0.000);AA +GA基因型(n=249)患者血浆MMP-3水平显著高于GG基因型患者[(241.71±81.73) ng/ml对(207.98±77.61) ng/ml;=4.336,P =0.000].结论 LAA或SAO患者血浆MMP-3水平增高,以LAA亚型增高最为显著;MMP-3Lys45Glu多态性可能与MMP-3血浆水平和LAA有关.
目的 探討基質金屬蛋白酶-3(matrix metalloprotemase-3,MMP-3)血漿水平及其基因Lys45Glu(rs679620)多態性與缺血性卒中及其TOAST亞型的關繫.方法 納入根據TOAST病因學分型分為大動脈粥樣硬化性卒中(large artery atherosclerotic stroke,LAA)和小動脈閉塞性卒中(small artery occlusion stroke,SAO)患者(缺血性卒中組)和健康體檢者(對照組).採用酶聯免疫吸附法檢測血漿MMP-3水平,多聚酶鏈反應-限製性片段長度多態性法檢測MMP-3 Lys45 Glu基因型.結果共納入急性缺血性卒中患者233例,其中LAA 162例,SAO 71例;200名健康體檢者作為對照組.缺血性卒中組血漿MMP-3水平顯著高于對照組[(253.99±75.02)ng/ml對(196.38±78.17) ng/ml;=7.813,P=0.000];LAA組[(262.81±69.23)ng/ml]血漿MMP-3水平均顯著高于SAO組[(233.85±83.90)ng/ml,P=0.008]和對照組(P=0.000),SAO組也顯著高于對照組(P=0.000).多變量logistic迴歸分析顯示,血清MMP-3水平增高是缺血性卒中的獨立危險因素[優勢比(odds ratio,OR)1.012,95％可信區間(confidence interval,CI)1.008～1.015;P=0.000].缺血性卒中組MMP-3Lys45Glu基因型(x2 =2.085,P=0.353)和等位基因(x2 =2.29,P=0.130)頻率與對照組均無顯著差異.LAA、SAO和對照組之間MMP-3 Lys45Glu基因型頻率存在顯著差異(x2=10.39,P=0.034),其中LAA組AA +GA頻率顯著高于SAO組(65.4％對49.3％;x2=5.375,P=0.020)和對照組(65.4％對54.0％;x2 =4.84,P=0.028);3組間MMP-3 Lys45Glu等位基因頻率無顯著性差異(x2=3.887,P=0.143).多變量logistic迴歸分析顯示,Lys45Glu多態性是LAA的獨立危險因素(OR1.783,95％ CI1.183 ～2.688;P=0.006).AA基因型(n=73)、GA基因型(n=176)和GG基因型(n=184)患者血漿MMP-3水平分彆為(235.70±70.85)ng/ml、(244.20±85.90) ng/ml和(207.98±77.61) ng/ml,3組間存在顯著性差異(F=9.682,P=0.000);AA +GA基因型(n=249)患者血漿MMP-3水平顯著高于GG基因型患者[(241.71±81.73) ng/ml對(207.98±77.61) ng/ml;=4.336,P =0.000].結論 LAA或SAO患者血漿MMP-3水平增高,以LAA亞型增高最為顯著;MMP-3Lys45Glu多態性可能與MMP-3血漿水平和LAA有關.
목적 탐토기질금속단백매-3(matrix metalloprotemase-3,MMP-3)혈장수평급기기인Lys45Glu(rs679620)다태성여결혈성졸중급기TOAST아형적관계.방법 납입근거TOAST병인학분형분위대동맥죽양경화성졸중(large artery atherosclerotic stroke,LAA)화소동맥폐새성졸중(small artery occlusion stroke,SAO)환자(결혈성졸중조)화건강체검자(대조조).채용매련면역흡부법검측혈장MMP-3수평,다취매련반응-한제성편단장도다태성법검측MMP-3 Lys45 Glu기인형.결과공납입급성결혈성졸중환자233례,기중LAA 162례,SAO 71례;200명건강체검자작위대조조.결혈성졸중조혈장MMP-3수평현저고우대조조[(253.99±75.02)ng/ml대(196.38±78.17) ng/ml;=7.813,P=0.000];LAA조[(262.81±69.23)ng/ml]혈장MMP-3수평균현저고우SAO조[(233.85±83.90)ng/ml,P=0.008]화대조조(P=0.000),SAO조야현저고우대조조(P=0.000).다변량logistic회귀분석현시,혈청MMP-3수평증고시결혈성졸중적독립위험인소[우세비(odds ratio,OR)1.012,95％가신구간(confidence interval,CI)1.008～1.015;P=0.000].결혈성졸중조MMP-3Lys45Glu기인형(x2 =2.085,P=0.353)화등위기인(x2 =2.29,P=0.130)빈솔여대조조균무현저차이.LAA、SAO화대조조지간MMP-3 Lys45Glu기인형빈솔존재현저차이(x2=10.39,P=0.034),기중LAA조AA +GA빈솔현저고우SAO조(65.4％대49.3％;x2=5.375,P=0.020)화대조조(65.4％대54.0％;x2 =4.84,P=0.028);3조간MMP-3 Lys45Glu등위기인빈솔무현저성차이(x2=3.887,P=0.143).다변량logistic회귀분석현시,Lys45Glu다태성시LAA적독립위험인소(OR1.783,95％ CI1.183 ～2.688;P=0.006).AA기인형(n=73)、GA기인형(n=176)화GG기인형(n=184)환자혈장MMP-3수평분별위(235.70±70.85)ng/ml、(244.20±85.90) ng/ml화(207.98±77.61) ng/ml,3조간존재현저성차이(F=9.682,P=0.000);AA +GA기인형(n=249)환자혈장MMP-3수평현저고우GG기인형환자[(241.71±81.73) ng/ml대(207.98±77.61) ng/ml;=4.336,P =0.000].결론 LAA혹SAO환자혈장MMP-3수평증고,이LAA아형증고최위현저;MMP-3Lys45Glu다태성가능여MMP-3혈장수평화LAA유관.
Objective To investigate the correlation of plasma matrix metalloproteinase-3 (MMP-3)levels and MMP-3 Lys45Glu (rs679620) polyrnorphism with ischemic stroke and its TOAST subtypes.Methods The patients with large artery atherosclerotic stroke (LAA) and small artery occlusion stroke (SAO)according to TOAST etiological typing (ischemic stroke group) and healthy subjects (control group) were enrolled.The enzyme-linked immunosorbent assay was used to detect plasma MMP-3 level.The polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect the genotypes of MMP-3 Lys45Glu.Results A total of 233 patients with ischemic stroke were enrolled,in which 162 were LAA and 71 were SAO; 200 healthy subjects were taken as controls.The plasma MMP-3 level in the ischernic stroke group was significantly higher than that in the control goup (253.99 ± 75.02 ng/ml vs.196.38 ± 78.17 ng/ml;t =7.813,P=0.000).The plasma MMP-3 level in the LAA group (262.81 ±69.23 ng/ml) was significantly higher than those in thegroups of SAO (233.85 ± 83.90 ng/ml,P =0.008) and control (P =0.000),and the plasma MMP-3 level in the SAO was also significantly higher than that in the control group (P =0.000).Multivariate logistic regression analysis showed that the increased serum MMP-3 level was an independent risk factor for ischemic stroke (odds ratio [OR] 1.012,95％ confidence interval [CI] 1.008-1.015; P =0.000).There was no significant difference in the frequencies of genotype (x2 =2.085,P =0.353) and allele (x2 =2.29,P =0.130) of MMP-3 Lys45Glu between the ischemic stroke group and the control group.However,there were significant difference in MMP-3 Lys45Glu genotype frequencies among.the groups of LAA,SAO and control (x2 =10.39,P=0.034).The AA + GA genotype frequency in the LAA group was significant higher than those in the groups of SAO (65.4％ vs.49.3％ ;x2 =5.375,P =0.020) and control (65.4％ vs.54.0％ ;x2 =4.84,P =0.028).There was no significant difference in the allele frequencies among the groups of LAA,SAO and control (x2 =3.887,P =0.143).Multivariate logistic regression analysis showed that MMP-3 Lys45Glu polymorphism was an independent risk factor for LAA (OR 1.783,95％ CI 1.183-2.688; P =0.006).The plasma MMP-3 level in patients with the genotypes AA (n =73),GA (n =176) and GG (n =184)were 235.70 ± 70.85 ng/ml,(244.20 ± 85.90 ng/ml and 207.98 ± 77.61 ng/ml.There were significant difference in the plasma MMP-3 levels among the patients with the genotypes AA,GA and GG (F=9.682,P =0.000).The plasma MMP-3 level in the patients with the genotype AA + GA was significantly higher than that in patients with genotype GG (241.71 ± 81.73 ng/ml vs.207.98 ± 77.61 ng/ml; t =4.336,P =0.000).Conclusions The plasma MMP-3 level increased in patients with LAA or SAO,especially in the patients with LAA.The MMP-3 Lys45Glu polymorphism might be associated with the plasma MMP-3 level and LAA.
