国际脑血管病杂志
國際腦血管病雜誌
국제뇌혈관병잡지
INTERNATIONAL JOURNAL OF CEREBROVASCULAR DISEASES
2013年
12期
892-897
,共6页
蓝琳芳%范玉华%郑璐%杨娴
藍琳芳%範玉華%鄭璐%楊嫻
람림방%범옥화%정로%양한
噻唑烷二酮类%吡格列酮%过氧化物酶体增生物激活受体γ%高血压%脑白质病%认知障碍%大鼠
噻唑烷二酮類%吡格列酮%過氧化物酶體增生物激活受體γ%高血壓%腦白質病%認知障礙%大鼠
새서완이동류%필격렬동%과양화물매체증생물격활수체γ%고혈압%뇌백질병%인지장애%대서
Thiazolidinediones%Pioglitazone%PPARγ%Hypertension%Leukoencephalopathies%Cognition Disorders%Rats
目的 探讨过氧化物酶体增殖物激活受体γ激动剂吡格列酮对慢性高血压大鼠脑白质病变和空间记忆功能的影响.方法 雄性Sprague-Dawley大鼠36只,随机分为假手术组(n=6)、高血压组(n=15)和吡格列酮组(n=15).高血压组和吡格列酮组采用双肾双夹法制作易卒中型肾血管性高血压大鼠(stroke-prone renovascular hypertensive rat,RHRSP)模型,术后8周开始灌胃给药.吡格列酮组给予10 mg/(kg·d)吡格列酮,高血压组给予等量生理盐水,分别于术前、给药前及给药后每隔2周经尾动脉监测血压.连续给药12周后进行Loyez染色观察脑白质疏松程度,水迷宫试验检测空间记忆功能.结果 RHRSP大鼠造模后血压缓慢升高,显著性高于假手术组(P均=0.000),高血压组与吡格列酮组血压无显著性差异(P =0.897).水迷宫试验显示,假手术组和吡格列酮组逃避潜伏期显著性短于高血压组(P均＜0.05),三组大鼠跨越平台次数分别为(5.200±1.798)次、(4.560±1.592)次和(2.333±1.978)次,差异具有统计学意义(F=8.143,P=0.001),假手术组和吡格列酮组均显著性多于高血压组(P均＜0.05).Loyez染色显示,假手术组、高血压组和吡格列酮组胼胝体脑白质病变分级分别为0.333±0.516、2.600±0.507和0.500±0.522,三组间差异有统计学意义(F =25.652,P=0.000),假手术组和吡格列酮组分级均显著性低于高血压组(P均＜0.05).结论 PPAR-γ激动剂吡格列酮能通过减轻慢性高血压大鼠脑白质病变保护空间记忆功能.
目的 探討過氧化物酶體增殖物激活受體γ激動劑吡格列酮對慢性高血壓大鼠腦白質病變和空間記憶功能的影響.方法 雄性Sprague-Dawley大鼠36隻,隨機分為假手術組(n=6)、高血壓組(n=15)和吡格列酮組(n=15).高血壓組和吡格列酮組採用雙腎雙夾法製作易卒中型腎血管性高血壓大鼠(stroke-prone renovascular hypertensive rat,RHRSP)模型,術後8週開始灌胃給藥.吡格列酮組給予10 mg/(kg·d)吡格列酮,高血壓組給予等量生理鹽水,分彆于術前、給藥前及給藥後每隔2週經尾動脈鑑測血壓.連續給藥12週後進行Loyez染色觀察腦白質疏鬆程度,水迷宮試驗檢測空間記憶功能.結果 RHRSP大鼠造模後血壓緩慢升高,顯著性高于假手術組(P均=0.000),高血壓組與吡格列酮組血壓無顯著性差異(P =0.897).水迷宮試驗顯示,假手術組和吡格列酮組逃避潛伏期顯著性短于高血壓組(P均＜0.05),三組大鼠跨越平檯次數分彆為(5.200±1.798)次、(4.560±1.592)次和(2.333±1.978)次,差異具有統計學意義(F=8.143,P=0.001),假手術組和吡格列酮組均顯著性多于高血壓組(P均＜0.05).Loyez染色顯示,假手術組、高血壓組和吡格列酮組胼胝體腦白質病變分級分彆為0.333±0.516、2.600±0.507和0.500±0.522,三組間差異有統計學意義(F =25.652,P=0.000),假手術組和吡格列酮組分級均顯著性低于高血壓組(P均＜0.05).結論 PPAR-γ激動劑吡格列酮能通過減輕慢性高血壓大鼠腦白質病變保護空間記憶功能.
목적 탐토과양화물매체증식물격활수체γ격동제필격렬동대만성고혈압대서뇌백질병변화공간기억공능적영향.방법 웅성Sprague-Dawley대서36지,수궤분위가수술조(n=6)、고혈압조(n=15)화필격렬동조(n=15).고혈압조화필격렬동조채용쌍신쌍협법제작역졸중형신혈관성고혈압대서(stroke-prone renovascular hypertensive rat,RHRSP)모형,술후8주개시관위급약.필격렬동조급여10 mg/(kg·d)필격렬동,고혈압조급여등량생리염수,분별우술전、급약전급급약후매격2주경미동맥감측혈압.련속급약12주후진행Loyez염색관찰뇌백질소송정도,수미궁시험검측공간기억공능.결과 RHRSP대서조모후혈압완만승고,현저성고우가수술조(P균=0.000),고혈압조여필격렬동조혈압무현저성차이(P =0.897).수미궁시험현시,가수술조화필격렬동조도피잠복기현저성단우고혈압조(P균＜0.05),삼조대서과월평태차수분별위(5.200±1.798)차、(4.560±1.592)차화(2.333±1.978)차,차이구유통계학의의(F=8.143,P=0.001),가수술조화필격렬동조균현저성다우고혈압조(P균＜0.05).Loyez염색현시,가수술조、고혈압조화필격렬동조변지체뇌백질병변분급분별위0.333±0.516、2.600±0.507화0.500±0.522,삼조간차이유통계학의의(F =25.652,P=0.000),가수술조화필격렬동조분급균현저성저우고혈압조(P균＜0.05).결론 PPAR-γ격동제필격렬동능통과감경만성고혈압대서뇌백질병변보호공간기억공능.
Objective To investigate the effect of peroxisome proliferator-activated receptor γ (PPARγ) agonist pioglitazone on white matter lesions and spatial memory function in chronic hypertensive rats.Methods Thirty-six male Sprague-Dawley rats were randomly divided into a sham operation group (n =6),a hypertensive group,(n =15),and a pinglitazone group (n =15).A model of stroke-prone renovascular hypertensive rat (RHRSP) was induced by the two-kidney two clip method in the hypertensive group and pioglitazone group.At 8 weeks after procedure,the rats were administered intragastrically.10 mg/(kg · d) pioglitazone was given in the pioglitazone group and an equal volume of saline was given in the hypertensive group.Blood pressure was monitored via caudal artery every 2 weeks before procedure,before administration,and after administration,respectively.After 12 weeks of continuous administration,Loyez staining was used to observe the degree of leukoaraiosis,and Morris water maze test was used to detect spatial memory function.Results After modeling,the blood pressure of RHRSP increased gradually.It was significantly higher than the sham operation group (all P =0.001).There was no significant difference in blood pressure between the hypertensive group and the pioglitazone group (P =0.897).The Morris water maze test showed that escape latencies of the sham operation group and the pioglitazone group were significantly shorter than the hypertensive group (all P ＜0.05).The numbers of crossing hidden platform in the 3 groups of rats were 5.200 ± 1.798,4.560 ± 1.592,and 2.333 ± 1.978 times,respectively.There were significantly differences (F =8.143,P =0.001).Both the sham operation group and the pioglitazone group were significantly more than the hypertensive group (all P ＜0.05).Loyez staining showed that the grades of white matter lesions in corpus callosum of the sham operation group,the hypertensive group,and the pioglitazone group were 0.333 ± 0.516,2.600 ± 0.507,and 0.500 ± 0.522,respectively.There were significantly differences among the 3 groups (F =25.652,P =0.000).The grade of the sham operation group and the pioglitazone group was significantly lower than the hypertensive group (all P＜ 0.05).Conclusions PPARγ agonist pioglitazone may protect the spatial memory function by relieving white matter lesions of the chronic hypertensive rats.
