国际脑血管病杂志
國際腦血管病雜誌
국제뇌혈관병잡지
INTERNATIONAL JOURNAL OF CEREBROVASCULAR DISEASES
2013年
12期
898-902
,共5页
卢昌均%韦冰心%安红伟%陆兵勋%刘国成%周哲屹
盧昌均%韋冰心%安紅偉%陸兵勛%劉國成%週哲屹
로창균%위빙심%안홍위%륙병훈%류국성%주철흘
痴呆,血管性%记忆障碍%乙酰胆碱%海马%细胞凋亡%疾病模型,动物%大鼠
癡呆,血管性%記憶障礙%乙酰膽堿%海馬%細胞凋亡%疾病模型,動物%大鼠
치태,혈관성%기억장애%을선담감%해마%세포조망%질병모형,동물%대서
Dementia,Vascular%Memory Disorders%Acetylcholine%Hippocampus%Apoptosis%Disease Models,Animal%Rats
目的 探讨血管性痴呆(vascular dementia,VaD)模型大鼠海马乙酰胆碱(acetylcholine, ACh)含量与海马CAl区神经元凋亡以及空间记忆障碍的相关性.方法 40只健康雄性Sprague-Dawley大鼠随机分为VaD组和假手术组,每组20只.间断夹闭颈总动脉法建立VaD模型.微透析法采集大鼠海马区透析液,高效液相色谱分析检测透析液ACh含量.Morris水迷宫实验测试其空间记忆能力.TUNEL染色法检测海马CA1区神经元凋亡.结果 微透析分析显示,VaD组海马区ACh含量较假手术组显著性降低[(0.442±0.028) μmmol/L对(1.560±0.092) μmmol/L；t=51.697,P=0.000].TUNEL染色显示,VaD组海马CA1区细胞凋亡率显著性高于假手术组[(55.652±2.051)％对(6.530±1.872)％;t =79.114,P=0.000].不同检测时间点逃避潜伏期均显著性延长[第3天时:(49.713±18.161)s对(13.322±2.454)s,t=-8.881,P=0.000)；第4天时:(34.368±7.424)s对(10.503±1.415)s,t=-14.121,P=0.000；第5天时:(30.676±6.669)s对(7.311±1.534)s,t=-15.270,P=0.000],穿越平台次数显著性减少[(3.768±1.072)次对(10.218±1.165)次；=18.224;P=0.000].Pearson相关分析显示,VaD组海马ACh浓度与逃避潜伏期呈负相关(第3天时:r=-0.476,P=0.034；第4天时:r=-0.700,P=0.001；第5天时:r=-0.693,P=0.001),与穿越平台次数呈正相关性(r=0.689,P=0.001),与海马CA1区神经元凋亡率呈负相关(r=-0.271,P=0.031).结论 VaD模型大鼠海马CA1区ACh含量降低,神经元凋亡率升高,可能是VaD大鼠认知损害的机制之一.
目的 探討血管性癡呆(vascular dementia,VaD)模型大鼠海馬乙酰膽堿(acetylcholine, ACh)含量與海馬CAl區神經元凋亡以及空間記憶障礙的相關性.方法 40隻健康雄性Sprague-Dawley大鼠隨機分為VaD組和假手術組,每組20隻.間斷夾閉頸總動脈法建立VaD模型.微透析法採集大鼠海馬區透析液,高效液相色譜分析檢測透析液ACh含量.Morris水迷宮實驗測試其空間記憶能力.TUNEL染色法檢測海馬CA1區神經元凋亡.結果 微透析分析顯示,VaD組海馬區ACh含量較假手術組顯著性降低[(0.442±0.028) μmmol/L對(1.560±0.092) μmmol/L；t=51.697,P=0.000].TUNEL染色顯示,VaD組海馬CA1區細胞凋亡率顯著性高于假手術組[(55.652±2.051)％對(6.530±1.872)％;t =79.114,P=0.000].不同檢測時間點逃避潛伏期均顯著性延長[第3天時:(49.713±18.161)s對(13.322±2.454)s,t=-8.881,P=0.000)；第4天時:(34.368±7.424)s對(10.503±1.415)s,t=-14.121,P=0.000；第5天時:(30.676±6.669)s對(7.311±1.534)s,t=-15.270,P=0.000],穿越平檯次數顯著性減少[(3.768±1.072)次對(10.218±1.165)次；=18.224;P=0.000].Pearson相關分析顯示,VaD組海馬ACh濃度與逃避潛伏期呈負相關(第3天時:r=-0.476,P=0.034；第4天時:r=-0.700,P=0.001；第5天時:r=-0.693,P=0.001),與穿越平檯次數呈正相關性(r=0.689,P=0.001),與海馬CA1區神經元凋亡率呈負相關(r=-0.271,P=0.031).結論 VaD模型大鼠海馬CA1區ACh含量降低,神經元凋亡率升高,可能是VaD大鼠認知損害的機製之一.
목적 탐토혈관성치태(vascular dementia,VaD)모형대서해마을선담감(acetylcholine, ACh)함량여해마CAl구신경원조망이급공간기억장애적상관성.방법 40지건강웅성Sprague-Dawley대서수궤분위VaD조화가수술조,매조20지.간단협폐경총동맥법건립VaD모형.미투석법채집대서해마구투석액,고효액상색보분석검측투석액ACh함량.Morris수미궁실험측시기공간기억능력.TUNEL염색법검측해마CA1구신경원조망.결과 미투석분석현시,VaD조해마구ACh함량교가수술조현저성강저[(0.442±0.028) μmmol/L대(1.560±0.092) μmmol/L；t=51.697,P=0.000].TUNEL염색현시,VaD조해마CA1구세포조망솔현저성고우가수술조[(55.652±2.051)％대(6.530±1.872)％;t =79.114,P=0.000].불동검측시간점도피잠복기균현저성연장[제3천시:(49.713±18.161)s대(13.322±2.454)s,t=-8.881,P=0.000)；제4천시:(34.368±7.424)s대(10.503±1.415)s,t=-14.121,P=0.000；제5천시:(30.676±6.669)s대(7.311±1.534)s,t=-15.270,P=0.000],천월평태차수현저성감소[(3.768±1.072)차대(10.218±1.165)차；=18.224;P=0.000].Pearson상관분석현시,VaD조해마ACh농도여도피잠복기정부상관(제3천시:r=-0.476,P=0.034；제4천시:r=-0.700,P=0.001；제5천시:r=-0.693,P=0.001),여천월평태차수정정상관성(r=0.689,P=0.001),여해마CA1구신경원조망솔정부상관(r=-0.271,P=0.031).결론 VaD모형대서해마CA1구ACh함량강저,신경원조망솔승고,가능시VaD대서인지손해적궤제지일.
Objective To investigate the correlations between the hippocampal acetylcholine (ACh) content and neuronal apoptosis in the hippocampal CA1 region as well as the spatial memory impairment in a rat model of vascular dementia (VaD).Methods Forty male healthy Sprague-Dawley rats were randomly divided into either a VaD group or a sham operation group (n =20 in each group).A VaD model was induced by intermittently clipping common carotid artery.Microdialysis was used to collect dialysis solutions in rat hippocampus.High-performance liquid chromatographic analysis was used to detect the ACh content in the dialysis fluid.Morris water maze test was used to test their learning and memory abilities.TUNEL staining was used to detect neuronal apoptosis in the hippocampal CA1 region.Results Microdialysis analysis showed that the ACh content in the hippocampus in the VaD group was significantly lower than that in the sham operation group (0.442 ± 0.028 μmmol/L vs.1.560 ± 0.092 μ mmol/L; t =51.697,P =0.000).TUNEL staining showed that the apoptosis rate in the hippocampal CA1 region in the VaD group was significantly higher than that in the sham operation group (55.652％ ±2.051％ vs.6.530％ ± 1.872％ ; t =79.114,P=0.000).The escape latencies at different detection time points were prolonged significantly (At day 3:49.713 ± 18.161 s vs.13.322 ± 2.454 s; t =-8.881,P =0.000; at day 4:34.368 ± 7.424 s vs.10.503±1.415 s; t=-14.121,P=0.000; at day 5:30.676± 6.669s vs.7.311± 1.534 s; t=-15.270,P =0.000),and the numbers of cross platform were reduced significantly (3.768 ± 1.072 vs.10.218 ± 1.165; t =18.224,P=0.000).Pearson correlation analysis showed that the ACh contents in the VaD group were negatively correlated with the escape latencies (at day 3:r =-0.476,P =0.034; at day 4:r=-0.700,P=0.001; at day 5:r=-0.693,P=0.001).They were positively correlated with the numbers of cross platform (r =0.689,P =0.001),and negatively correlated with the neuronal apoptosis rates in the hippocampal CA1 region (r =-0.271,P =0.031).Conclusions The decreased ACh content,the increased neuronal apoptosis rate in the rat hippocampal CA1 region in the VaD model may be one of the mechanisms of cognitive impairment in VaD rats.
