国际脑血管病杂志
國際腦血管病雜誌
국제뇌혈관병잡지
INTERNATIONAL JOURNAL OF CEREBROVASCULAR DISEASES
2014年
9期
665-670
,共6页
曹修麗%萬玉杰%王軍%徐艷%李燕%朱冠南
曹脩麗%萬玉傑%王軍%徐艷%李燕%硃冠南
조수려%만옥걸%왕군%서염%리연%주관남
缺氧缺血,脑%氧调节蛋白%转录因子CHOP%调节因子X转录因子%基因表达%内质网%参附注射液%大鼠
缺氧缺血,腦%氧調節蛋白%轉錄因子CHOP%調節因子X轉錄因子%基因錶達%內質網%參附註射液%大鼠
결양결혈,뇌%양조절단백%전록인자CHOP%조절인자X전록인자%기인표체%내질망%삼부주사액%대서
Hypoxia-Ischemia,Brain%Regulatory Factor Ⅹ Transcription Factors%Transcription Factor CHOP%Oxygen-Regulated Proteins%Gene Expression%Endoplasmic Reticulum%Shen Fu Zhu She Ye%Rats
目的 探讨参附注射液对新生大鼠缺氧缺血性脑损伤(hypoxic-ischemic brain damage,HIBD)后脑皮质神经元内质网应激相关因子氧调节蛋白150(oxygen regulate protein 150,ORP150)、X盒结合蛋白1(X-box binding protein1,XBP1)和C/EBP同源蛋白(C/EBP homologous protein,CHOP)mRNA表达的影响.方法 新生7d的Sprague-Dawley大鼠随机分为假手术组、生理盐水对照组与参附治疗组(参附注射液,10 ml/kg,腹腔注射,1次/d,连续3d).按照造模后不同观察时间点又分为3h、6h、12 h、24 h、3d、7d6个亚组,每个亚组8只.建立新生大鼠HIBD模型.采用逆转录聚合酶链反应检测大鼠大脑皮质ORP150、XBP1和CHOP mRNA表达.结果 假手术组ORP150、XBP1和CHOP基因表达很弱.生理盐水对照组和参附治疗组ORP150、XBP1和CHOP mRNA表达在模型制作后3h即较假手术组显著性上调(P均＜0.05);XBP1和CHOP mRNA表达分别在6h和12 h时达高峰,之后均有所下降,7d时接近假手术组水平.其中,参附治疗组XBP1和CHOP mRNA表达在模型制作后12 h、24 h和3d时显著性低于生理盐水对照组(P均＜0.05);生理盐水对照组XBP1 mRNA表达与CHOP mRNA表达呈显著正相关(r=0.649,P＜0.05).结论 新生大鼠HIBD可诱发内质网应激反应,ORP150、XBP1和CHOP可能参与了新生大鼠HIBD后迟发性神经元损伤过程.参附注射液可下调XBP1和CHOP mRNA表达,抑制内质网应激反应.
目的 探討參附註射液對新生大鼠缺氧缺血性腦損傷(hypoxic-ischemic brain damage,HIBD)後腦皮質神經元內質網應激相關因子氧調節蛋白150(oxygen regulate protein 150,ORP150)、X盒結閤蛋白1(X-box binding protein1,XBP1)和C/EBP同源蛋白(C/EBP homologous protein,CHOP)mRNA錶達的影響.方法 新生7d的Sprague-Dawley大鼠隨機分為假手術組、生理鹽水對照組與參附治療組(參附註射液,10 ml/kg,腹腔註射,1次/d,連續3d).按照造模後不同觀察時間點又分為3h、6h、12 h、24 h、3d、7d6箇亞組,每箇亞組8隻.建立新生大鼠HIBD模型.採用逆轉錄聚閤酶鏈反應檢測大鼠大腦皮質ORP150、XBP1和CHOP mRNA錶達.結果 假手術組ORP150、XBP1和CHOP基因錶達很弱.生理鹽水對照組和參附治療組ORP150、XBP1和CHOP mRNA錶達在模型製作後3h即較假手術組顯著性上調(P均＜0.05);XBP1和CHOP mRNA錶達分彆在6h和12 h時達高峰,之後均有所下降,7d時接近假手術組水平.其中,參附治療組XBP1和CHOP mRNA錶達在模型製作後12 h、24 h和3d時顯著性低于生理鹽水對照組(P均＜0.05);生理鹽水對照組XBP1 mRNA錶達與CHOP mRNA錶達呈顯著正相關(r=0.649,P＜0.05).結論 新生大鼠HIBD可誘髮內質網應激反應,ORP150、XBP1和CHOP可能參與瞭新生大鼠HIBD後遲髮性神經元損傷過程.參附註射液可下調XBP1和CHOP mRNA錶達,抑製內質網應激反應.
목적 탐토삼부주사액대신생대서결양결혈성뇌손상(hypoxic-ischemic brain damage,HIBD)후뇌피질신경원내질망응격상관인자양조절단백150(oxygen regulate protein 150,ORP150)、X합결합단백1(X-box binding protein1,XBP1)화C/EBP동원단백(C/EBP homologous protein,CHOP)mRNA표체적영향.방법 신생7d적Sprague-Dawley대서수궤분위가수술조、생리염수대조조여삼부치료조(삼부주사액,10 ml/kg,복강주사,1차/d,련속3d).안조조모후불동관찰시간점우분위3h、6h、12 h、24 h、3d、7d6개아조,매개아조8지.건립신생대서HIBD모형.채용역전록취합매련반응검측대서대뇌피질ORP150、XBP1화CHOP mRNA표체.결과 가수술조ORP150、XBP1화CHOP기인표체흔약.생리염수대조조화삼부치료조ORP150、XBP1화CHOP mRNA표체재모형제작후3h즉교가수술조현저성상조(P균＜0.05);XBP1화CHOP mRNA표체분별재6h화12 h시체고봉,지후균유소하강,7d시접근가수술조수평.기중,삼부치료조XBP1화CHOP mRNA표체재모형제작후12 h、24 h화3d시현저성저우생리염수대조조(P균＜0.05);생리염수대조조XBP1 mRNA표체여CHOP mRNA표체정현저정상관(r=0.649,P＜0.05).결론 신생대서HIBD가유발내질망응격반응,ORP150、XBP1화CHOP가능삼여료신생대서HIBD후지발성신경원손상과정.삼부주사액가하조XBP1화CHOP mRNA표체,억제내질망응격반응.
Objective To investigate the effect of Shenfu injection on the expressions of endoplasmic reticulum stress-related factors oxygen-regulated protein 150 (ORP150),X-box binding protein 1 (XBP1),and C/EBP homologous protein (CHOP) mRNAs in cerebral cortical neurons after hypoxic-ischemic brain damage (HIBD) in neonatal rats.Methods The 7 day-newborn Sprague-Dawley rats were randomly divided into sham operation,normal saline and Shenfu treatment (Shenfu injection,10 ml/kg per day,peritoneal injection,for 3 days) groups.They were redivided into 3 h,6 h,12 h,24 h,3 d and 7 d subgroups at different time points after modeling (n =8 in each group).A HIBD model of the neonatal rats was induced.Reverse transcription-polymerase chain reaction was used to detect the expressions of ORP150,XBP1,and CHOP mRNAs in rat cerebral cortex.Results The expressions of ORP150,XBP1 and CHOP mRNAs of the sham operation group was very week.The expressions of ORP150,XBP1 and CHOP mRNAs in the normal saline group and the Shenfu treatment group were significantly upregulated compared to those in the sham operation group at 3 h after modeling (all P ＜0.05); the expressions of XBP1 and CHOP mRNAs reached the peak at 6 and 12 h,respectively.Then they decreased mildly and closed to the level in the sham operation group at day 7.The expressions of XBP1 and CHOP mRNAs in the Shenfu treatment group at 12,24 h and day 3 after modeling were significantly lower than those in the normal saline group (all P ＜0.05).The XBP1 mRNA expression was significantly positively correlated with the CHOP mRNA expression in the normal saline group (r =0.649,P ＜0.05).Conclusions HIBD in neonatal rats induces endoplasmic reticulum stress response.ORP150,XBP1 and CHOP may be involved in the delayed neuronal damage process after HIBD in neonatal rats.Shenfu injection downregulates the expression of XBP1 and CHOP mRNAs and inhibits endoplasmic reticulum stress response.
