CAJ | 학술논문

目的通过TREM-1基因在重症急性胰腺炎大鼠胰腺、肺组织中的基因表达,探讨TREM-1基因在重症急性胰腺炎发病机制中的作用,进而为治疗重症急性胰腺炎提供新的分子靶点.方法健康雄性Wistar大鼠30 只,完全随机分为两组,每组15只.A组:正常组；B组:重症急性胰腺炎组；用5％牛黄胆酸钠逆行胰胆管注射制作重症急性胰腺炎模型.结果 TREM-1相对表达量比较:Real-time PCR进行相对定量检测大鼠胰腺、肺组织中TREM-1的相对表达量,B组胰腺、肺两个组织中TREM-1 mRNA相对含量的在6h、12 h 24 h相应时间点相比明显高于A组,差异有显著性统计学意义(P＜0.05),且随时间变化逐渐升高；且与胰腺病理变化呈正相关.结论重症急性胰腺炎时,TREM-1的相对表达量在大鼠胰腺、肺组织中的明显表达,更能加重胰腺损伤及全身炎症反应综合征.
목적 통과TREM-1기인재중증급성이선염대서이선、폐조직중적기인표체,탐토TREM-1기인재중증급성이선염발병궤제중적작용,진이위치료중증급성이선염제공신적분자파점.방법 건강웅성Wistar대서30 지,완전수궤분위량조,매조15지.A조:정상조；B조:중증급성이선염조；용5％우황담산납역행이담관주사제작중증급성이선염모형.결과 TREM-1상대표체량비교:Real-time PCR진행상대정량검측대서이선、폐조직중TREM-1적상대표체량,B조이선、폐량개조직중TREM-1 mRNA상대함량적재6h、12 h 24 h상응시간점상비명현고우A조,차이유현저성통계학의의(P＜0.05),차수시간변화축점승고；차여이선병리변화정정상관.결론 중증급성이선염시,TREM-1적상대표체량재대서이선、폐조직중적명현표체,경능가중이선손상급전신염증반응종합정.
Objective Through detecting the expression of triggering receptor expressed on myeloid cells-1 (TREM-1) in pancreas and lung of rats with severe acute panereatitis,to approach the role of TREM-1 in severe actute pancreatitis pathogenesis,and provid a new molecular target for SAP.Methods Thirty healthy male Wistar rat were randomly divided into two groups:control group(group A),n =15 ; severe acute pancreatitis group (group B),n =15.The model of severe acute pancreatitis of rats was induced by retrograde injection of 5％ sodium taurocholate into the pancreatic duct.Results TREM-1 in pancreatic tissues and lung tissues was significantly higher in group B than group A in three time points(6 h,12 h,24 h) and had significant changes(P ＜ 0.05),and had a positive correlation with pancreatic pathology.Conclusion TREM-1 was significantly expressed in severe acute pancreatitis,and aggravated pancreas damage and systemic inflammatory response syndrome.