国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2013年
3期
160-164
,共5页
姜雨刚%张安秦%朱彩霞%卢奕嘉%王颀
薑雨剛%張安秦%硃綵霞%盧奕嘉%王頎
강우강%장안진%주채하%로혁가%왕기
乳腺肿瘤%癌%甲硫腺苷磷酸化酶%基质金属蛋白酶1%增生%侵袭%迁移
乳腺腫瘤%癌%甲硫腺苷燐痠化酶%基質金屬蛋白酶1%增生%侵襲%遷移
유선종류%암%갑류선감린산화매%기질금속단백매1%증생%침습%천이
Breast neoplasms%Carcinoma%Methylthioadenosine phosphorylase%Matrix metalloproteinase 1%Hyperplasia%Invasion%Migration
目的 观察甲硫腺苷磷酸化酶(MTAP)对人乳腺癌细胞侵袭和迁移能力的影响.方法 利用RNA干扰技术下调人乳腺癌细胞系MCF-7细胞中MTAP的表达,CCK-8试剂盒检测细胞增生能力,Transwell检测MCF-7细胞的侵袭及迁移能力.Western blotting检测MCF-7细胞中MTAP及基质金属蛋白酶l(MMPl)的表达.实验分组:空白对照组、阴性对照组、MTAP-siRNA实验组.结果 RNA干扰技术下调MCF-7细胞中MTAP表达后,促进了MCF-7细胞的增生,CCK-8检测显示,MATP-siRNA实验组450 nm吸光度在24 h、48 h、72 h分别是空白对照组的(112.3±11.9)％、(144.4±8.4)％、(169.3±9.4)％.Transwell侵袭实验结果显示,空白对照组、阴性对照组及MTAP-siRNA实验组在570 nm吸光度值分别为0.49±0.06、0.45 ±0.07、0.87±0.07.细胞迁移实验显示空白对照组、阴性对照组及MTAP-siRNA实验组在570 nm吸光度值分别为0.46 ±0.06、0.49 ±0.08、0.75±0.07.在下调MCF-7细胞中MTAP的表达后,Western blotting检测发现MMP1的表达上调.结论 MTAP表达下调可以增强人乳腺癌细胞MCF-7的侵袭及迁移能力,并可能与MMP1的表达上调有关.
目的 觀察甲硫腺苷燐痠化酶(MTAP)對人乳腺癌細胞侵襲和遷移能力的影響.方法 利用RNA榦擾技術下調人乳腺癌細胞繫MCF-7細胞中MTAP的錶達,CCK-8試劑盒檢測細胞增生能力,Transwell檢測MCF-7細胞的侵襲及遷移能力.Western blotting檢測MCF-7細胞中MTAP及基質金屬蛋白酶l(MMPl)的錶達.實驗分組:空白對照組、陰性對照組、MTAP-siRNA實驗組.結果 RNA榦擾技術下調MCF-7細胞中MTAP錶達後,促進瞭MCF-7細胞的增生,CCK-8檢測顯示,MATP-siRNA實驗組450 nm吸光度在24 h、48 h、72 h分彆是空白對照組的(112.3±11.9)％、(144.4±8.4)％、(169.3±9.4)％.Transwell侵襲實驗結果顯示,空白對照組、陰性對照組及MTAP-siRNA實驗組在570 nm吸光度值分彆為0.49±0.06、0.45 ±0.07、0.87±0.07.細胞遷移實驗顯示空白對照組、陰性對照組及MTAP-siRNA實驗組在570 nm吸光度值分彆為0.46 ±0.06、0.49 ±0.08、0.75±0.07.在下調MCF-7細胞中MTAP的錶達後,Western blotting檢測髮現MMP1的錶達上調.結論 MTAP錶達下調可以增彊人乳腺癌細胞MCF-7的侵襲及遷移能力,併可能與MMP1的錶達上調有關.
목적 관찰갑류선감린산화매(MTAP)대인유선암세포침습화천이능력적영향.방법 이용RNA간우기술하조인유선암세포계MCF-7세포중MTAP적표체,CCK-8시제합검측세포증생능력,Transwell검측MCF-7세포적침습급천이능력.Western blotting검측MCF-7세포중MTAP급기질금속단백매l(MMPl)적표체.실험분조:공백대조조、음성대조조、MTAP-siRNA실험조.결과 RNA간우기술하조MCF-7세포중MTAP표체후,촉진료MCF-7세포적증생,CCK-8검측현시,MATP-siRNA실험조450 nm흡광도재24 h、48 h、72 h분별시공백대조조적(112.3±11.9)％、(144.4±8.4)％、(169.3±9.4)％.Transwell침습실험결과현시,공백대조조、음성대조조급MTAP-siRNA실험조재570 nm흡광도치분별위0.49±0.06、0.45 ±0.07、0.87±0.07.세포천이실험현시공백대조조、음성대조조급MTAP-siRNA실험조재570 nm흡광도치분별위0.46 ±0.06、0.49 ±0.08、0.75±0.07.재하조MCF-7세포중MTAP적표체후,Western blotting검측발현MMP1적표체상조.결론 MTAP표체하조가이증강인유선암세포MCF-7적침습급천이능력,병가능여MMP1적표체상조유관.
Objective To investigate the effects of methylthioadenosine phosphorylase (MTAP) on invasion and migration in breast cancer cells.Methods Human breast cancer cell line MCF-7 cells were treated with MTAPtargeted siRNA to diminish MTAP mRNA.MCF-7 cells proliferation was evaluated by cell counting kit-8,the analysis of cells invasion and migration was performed using Transwell chamber.The expressions of MTAP and matrix metalloproteinase 1 (MMP1) in cell extracts were detected by Western blotting.The experimental divided into blank contrd group,negative control group,MTAP-siRNA experimental group.Results The MCF-7 cells growth was promoted after knockdown the MTAP mRNA.MATP-siRNA experimental group 450 nm absorbance values at 24h,48 hand72 h of the control group were (112.3±11.9)％,(144.4±8.4)％,(169.3±9.4)％ respectively.Cell invasion analysis by Transwell chamber showed 570 nm absorbance values were 0.49 ± 0.06 (control),0.45 ± 0.07 (negative control) and 0.87 ± 0.07 (MTAP-siRNA) respectively.Cell migration analysis by Transwell chamber showed 570 nm absorbance values were 0.46 ± 0.06 (control),0.49 ± 0.08 (negative control)and 0.75 ± 0.07 (MTAP-siRNA) respectively.The expression of MMP1 in MCF-7 cells was upregulated after knockdown the MTAP mRNA.Conclusion The knockdown of MTAP in MCF-7 cell can increase the cells invasion and migration,and this may involve the the MMP1.
