国际外科学杂志
國際外科學雜誌
국제외과학잡지
INTERNATIONAL JOURNAL OF SURGERY
2014年
6期
382-386,封3
,共6页
张同军%薛栋%刘敏%李新军%张成德%成丕光%巩本刚
張同軍%薛棟%劉敏%李新軍%張成德%成丕光%鞏本剛
장동군%설동%류민%리신군%장성덕%성비광%공본강
胰腺肿瘤%Twist转录因子%N-钙黏素%波形蛋白%上皮间质转化%免疫组织化学
胰腺腫瘤%Twist轉錄因子%N-鈣黏素%波形蛋白%上皮間質轉化%免疫組織化學
이선종류%Twist전록인자%N-개점소%파형단백%상피간질전화%면역조직화학
Pancreatic neoplasms%Twist transcription factor%N-cadherin%Vimentin%Epithelial mesenchymal transition%Immunohistochemistry
目的 探讨Twist介导的上皮间质转化在胰腺癌进展中的作用.方法 采用免疫组织化学法检测42例胰腺癌组织中Twist、N-cadherin及Vimentin蛋白的表达,26例癌旁组织作为对照.结果 胰腺癌组织中Twist、N-cadherin及Vimentin蛋白的阳性表达率分别为76.2％、59.5％和57.1％.Twist、N-cadherin 及Vimentin在26例癌组织及配对癌旁组织中的阳性表达率比较,差异均有统计学意义(均P＜0.001);Twist、N-cadherin及Vimentin的表达均与肿瘤分化程度、TNM分期、神经浸润及淋巴结转移明显相关(P＜0.05).经Spearman等级相关性分析,Twist与N-cadherin及Vimentin在胰腺癌组织中的表达均呈正相关(r =0.506,P=0.001;r=0.417,P=0.006);N-cadherin及Vimentin在胰腺癌组织中的表达呈正相关(r=0.462,P=0.002).结论 Twist可能通过诱导胰腺上皮细胞发生上皮间质转化促进胰腺癌侵袭转移.
目的 探討Twist介導的上皮間質轉化在胰腺癌進展中的作用.方法 採用免疫組織化學法檢測42例胰腺癌組織中Twist、N-cadherin及Vimentin蛋白的錶達,26例癌徬組織作為對照.結果 胰腺癌組織中Twist、N-cadherin及Vimentin蛋白的暘性錶達率分彆為76.2％、59.5％和57.1％.Twist、N-cadherin 及Vimentin在26例癌組織及配對癌徬組織中的暘性錶達率比較,差異均有統計學意義(均P＜0.001);Twist、N-cadherin及Vimentin的錶達均與腫瘤分化程度、TNM分期、神經浸潤及淋巴結轉移明顯相關(P＜0.05).經Spearman等級相關性分析,Twist與N-cadherin及Vimentin在胰腺癌組織中的錶達均呈正相關(r =0.506,P=0.001;r=0.417,P=0.006);N-cadherin及Vimentin在胰腺癌組織中的錶達呈正相關(r=0.462,P=0.002).結論 Twist可能通過誘導胰腺上皮細胞髮生上皮間質轉化促進胰腺癌侵襲轉移.
목적 탐토Twist개도적상피간질전화재이선암진전중적작용.방법 채용면역조직화학법검측42례이선암조직중Twist、N-cadherin급Vimentin단백적표체,26례암방조직작위대조.결과 이선암조직중Twist、N-cadherin급Vimentin단백적양성표체솔분별위76.2％、59.5％화57.1％.Twist、N-cadherin 급Vimentin재26례암조직급배대암방조직중적양성표체솔비교,차이균유통계학의의(균P＜0.001);Twist、N-cadherin급Vimentin적표체균여종류분화정도、TNM분기、신경침윤급림파결전이명현상관(P＜0.05).경Spearman등급상관성분석,Twist여N-cadherin급Vimentin재이선암조직중적표체균정정상관(r =0.506,P=0.001;r=0.417,P=0.006);N-cadherin급Vimentin재이선암조직중적표체정정상관(r=0.462,P=0.002).결론 Twist가능통과유도이선상피세포발생상피간질전화촉진이선암침습전이.
Objective To investigate the relationship of Twist induced epithelial mesenchymal transitions (EMT) during pancreatic carcinoma progression.Methods The expressions of Twist,N-cadherin and Vimentin proteins were tested by immunohistochemistry in 42 cases of pancreatic carcinomas and 26 cases of adjacent paracancerous tissues.Results The positive rates of Twist,N-cadherin and Vimentin in 42 cases of pancreatic carcinomas were 76.2％,59.5％ and 57.1％,respectively.The positive rate of Twist,N-cadherin and Vimentin were all significant between pancreatic carcinomas and paired tumor-adjacent paracancerous tissues (all P ＜0.001).The differences of the expression of Twist,N-cadherin and Vimentin in pancreatic carcinoma of tumor differentiation,TNM stage,neural invasion and lymph node metastasis were significant (all P ＜ 0.05).Significantly positive correlation was found between the expression of Twist and the others (N-cadherin and Vimentin) by using spearman correlation analysis (r =0.506,P =0.001 ; r =0.417,P =0.006).Significantly positive correlation was found between the expression of N-cadherin and Vimentin by using spearman correlation analysis(r =0.462,P =0.002).Conclusions Twist signaling pathway activation might mediate pancreatic epithelial cells to EMT and then promote pancreatic carcinoma invasion and metastasis.
