CAJ | 학술논문

目的探讨miR-145、miR-21、miR-10b在乳腺癌中的表达及意义,为进一步提高乳腺癌诊治水平提供理论实践基础.方法采用Real-time PCR法检测35例乳腺癌组织及其癌旁正常乳腺组织中miR-145、miR-21、miR-10b的表达,分析其与乳腺癌临床病理特征的关系;同时用免疫组化检查乳腺癌中ER、PR的表达;荧光原位杂交检测HER-2、TOP 2A的表达.结果 miR-145在乳腺癌组织中的表达低于癌旁正常组织,仅为正常组织的0.78倍,但差异无统计学意义,在肿块大小、淋巴结转移、ER阳性、HER-2阳性、TOP 2A阳性患者中低表达(P＜0.05),与年龄、月经、PR表达无关.miR-21、miR-10b在乳腺癌组织中的表达明显高于癌旁组织,分别是正常组织的8.58倍、1.70倍(P＜0.05).miR-21在年老、绝经、淋巴结转移、HER-2阳性患者中高表达(P＜0.05),与肿块大小、ER、PR、及TOP 2A的表达程度无关.miR-10b在肿块大小、淋巴结转移、TOP 2A阳性中高表达(P＜0.05),而与患者年龄、月经、ER、PR、HER-2等无关.结论 miR-145、miR-21、miR-10b在乳腺癌发展、侵袭的过程中可能发挥重要作用.
목적 탐토miR-145、miR-21、miR-10b재유선암중적표체급의의,위진일보제고유선암진치수평제공이론실천기출.방법 채용Real-time PCR법검측35례유선암조직급기암방정상유선조직중miR-145、miR-21、miR-10b적표체,분석기여유선암림상병리특정적관계;동시용면역조화검사유선암중ER、PR적표체;형광원위잡교검측HER-2、TOP 2A적표체.결과 miR-145재유선암조직중적표체저우암방정상조직,부위정상조직적0.78배,단차이무통계학의의,재종괴대소、림파결전이、ER양성、HER-2양성、TOP 2A양성환자중저표체(P＜0.05),여년령、월경、PR표체무관.miR-21、miR-10b재유선암조직중적표체명현고우암방조직,분별시정상조직적8.58배、1.70배(P＜0.05).miR-21재년로、절경、림파결전이、HER-2양성환자중고표체(P＜0.05),여종괴대소、ER、PR、급TOP 2A적표체정도무관.miR-10b재종괴대소、림파결전이、TOP 2A양성중고표체(P＜0.05),이여환자년령、월경、ER、PR、HER-2등무관.결론 miR-145、miR-21、miR-10b재유선암발전、침습적과정중가능발휘중요작용.
Objective To investigate the significance of miR-145,and miR-21,the role of miR-10b in breast cancer,providing theoretical and practical foundation for further improving the diagnosis and treatment of breast cancer.Methods Real-time RT-PCR detected 35 cases of breast cancer and adjacent normal breast tissue miR-145,miR-21,miR-10b expression,and analysis of their relationship with clinico-pathological features of breast cancer; the expression was examined by immunohistochemistry in breast cancer ER,PR expression; fluorescencein situ hybridization in detection of HER-2,TOP 2A.Results Expression of miR-145 in breast cancer tissues was lower than in adjacent normal tissue,only 0.78 of normal tissue,but without statistical significance.miR-145 in large lumps,lymph node metastasis,ER-positive,HER-2 positive,TOP 2A-positive patients with low expression (P ＜ 0.05),with age,menstruation,PR expression is independent.Expression of miR-21,miR-10b in breast cancer tissues was significantly higher than that in adjacent normal tissue,which are 8.58,1.70 times the normal tissues (P ＜0.05).MiR-21 in old age,menopause,lymph node metastasis,HER-2 positive patients with high expression (P ＜0.05),regardless of tumor size,ER,PR,and the degree of expression of TOP 2A; miR-10b in large lumps,lymph node metastasis,TOP 2A high positive expression (P ＜ 0.05),while with age,menstruation,ER,PR,HER-2 independent.MiR-10b in large lumps,lymph node metastasis,the positive expression TOP 2A high expression (P ＜ 0.05),and with age,unrelated to menstruation,ER,PR,HER-2 expression.Conclusions miR-145,miR-21 and miR-10b in breast cancer development,invasion process may play an important role.