CAJ | 학술논문

目的明确黑龙江省耐多药结核分枝杆菌耐药相关基因katG、rpoB和inhA的突变特征.方法收集黑龙江省肺结核患者痰培养的1 50株耐多药以及35株对一线药物敏感的结核分枝杆菌;应用PCR法扩增菌株的katG、rpoB及inhA基因,并进行序列测定;应用DNAMAN 6.0.3.99软件分析耐药基因核苷酸及氨基酸突变情况.结果该地区耐多药结核分枝杆菌katG基因优势突变位点为S315,占全部katG基因突变菌株的90.3％;inhA启动子区突变以C-15T为主,占85.4％;rpoB基因突变位点以S531为主,占72.5％;其次是S526突变,占19.7％;katG联合inhA突变的菌株有6株(4.0％),其中5株(3.3％)有3种基因突变.上述耐药基因优势突变类型的比例与国内其他地区有一定的差异.结论该地区耐多药结核分枝杆菌耐药相关基因优势突变类型与国内其他地区无明显差异,但优势突变位点的构成比与国内其他地区不同.
목적 명학흑룡강성내다약결핵분지간균내약상관기인katG、rpoB화inhA적돌변특정.방법 수집흑룡강성폐결핵환자담배양적1 50주내다약이급35주대일선약물민감적결핵분지간균;응용PCR법확증균주적katG、rpoB급inhA기인,병진행서렬측정;응용DNAMAN 6.0.3.99연건분석내약기인핵감산급안기산돌변정황.결과 해지구내다약결핵분지간균katG기인우세돌변위점위S315,점전부katG기인돌변균주적90.3％;inhA계동자구돌변이C-15T위주,점85.4％;rpoB기인돌변위점이S531위주,점72.5％;기차시S526돌변,점19.7％;katG연합inhA돌변적균주유6주(4.0％),기중5주(3.3％)유3충기인돌변.상술내약기인우세돌변류형적비례여국내기타지구유일정적차이.결론 해지구내다약결핵분지간균내약상관기인우세돌변류형여국내기타지구무명현차이,단우세돌변위점적구성비여국내기타지구불동.
Objective To investigate the mutation characteristics of drug resistance genes of multidrug-resistant M.tuberculosis in Heilongjiang province.Methods 150 muhidmg-resistant and 35 drug-sensitive M.tuberculosis isolates were obtained from clinical specimens of pulmonary tuberculosis patients in Heilongjiang province.The katG,rpoB,and inhA genes were amplified by PCR and sequenced.The mutation of genes and the changes of amino acids were analyzed by DNAMAN 6.0.3.99.Results Among the muhidrug-resistant M.tuberculosis isolates in the studied area,mutation at S315,accounting for 90.3％ of all mutation types in katG gene,are the dominant.C-15T in inhA promoter region accounted for 85.4％ of all mutation types.Of the muhidrug-resistant M.tuberculosis isolates containing rpoB gene mutations,72.5％ have S531 mutation; 19.7％ have S526 mutation.Six isolates (4.0 ％) have combined mutations in katG and inhA genes ; Five isolates (3.3 ％) have combined mutations in three genes.The proportions of dominant mutations of the drug resistant genes in this area are varied comparing with those in other areas of China.Conclusion There are the similar mutation types but different proportions of dominant mutations of the genes associated with drug-resistant in the multidrugresistant M.tuberculosis isolates in Heilongjiang Province comparing with other areas of China.