国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2014年
1期
6-12
,共7页
智艳芳%李肖甫%沈勇%邱翠%荣守华%张新
智豔芳%李肖甫%瀋勇%邱翠%榮守華%張新
지염방%리초보%침용%구취%영수화%장신
HPV%E6/E7 mRNA%宫颈病变%bDNA法
HPV%E6/E7 mRNA%宮頸病變%bDNA法
HPV%E6/E7 mRNA%궁경병변%bDNA법
Human papillomavirus%E6/E7 mRNA%Cervical lesion%bDNA assay
目的 探讨人乳头瘤病毒(HPV)癌基因E6/E7 mRNA检测在宫颈病变中的临床意义.方法 收集郑州大学第三附属医院行宫颈液基薄层细胞学(TCT)检查,且经第二代杂交捕获法(HC2)证实HPV DNA阳性的患者280例,采用QUANTIVIRUS(a) HPV E6/E7 mRNA3.0 Assay(bDNA)法检测宫颈脱落细胞中HPV癌基因E6/E7 mRNA水平,并随访其组织病理结果.结果 280例患者的细胞学结果:未见上皮内病变或恶性细胞(NILM) 27例(9.64％),非典型鳞状上皮细胞(ASC) 94例(33.57％),低度鳞状上皮内病变(LSIE)70例(25.00％),高度鳞状上皮内病变(HSIL) 67例(23.93％),鳞癌(SCC) 22例(7.86％).不同细胞学诊断级别间HPV癌基因E6/E7 mRNA水平比较,差异具有统计学意义(F=2.827,P=0.025),病变组(ASC、LSIL、HSIL、SCC) HPV癌基因E6/E7 mRNA水平均显著高于NILM组(*P=0.000、**P=0.000、***P=0.016、****P=0.022).随宫颈细胞学病变程度加重,HPV癌基因E6/E7 mRNA表达增高;280例患者有1 84例追踪到组织学结果:慢性宫颈炎51例(27.72％),宫颈上皮内瘤样病变Ⅰ级(CIN Ⅰ)46例(25.00％),宫颈上皮内瘤样病变Ⅱ级(CINⅡ)25例(13.59％),宫颈上皮内瘤样病变Ⅲ级(CINⅢ)30例(16.30％),SCC 32例(17.39％).不同组织学诊断级别组间HPV癌基因E6/E7 mRNA水平比较,差异显著(F=2.682,P=0.033),病变组(CIN Ⅰ、CINⅡ、CINⅢ、SCC) HPV癌基因E6/E7 mRNA水平均显著高于慢炎组(*P=0.016、**P=0.025、***P=0.007、**** P=0.039).随宫颈组织学病变程度加重,HPV癌基因E6/E7 mRNA表达水平增高;将细胞学病变程度和组织学病变程度分别与HPV癌基因E6/E7mRNA水平进行spearman等级相关分析,相关系数r分别为0.188(P=0.001)与0.582 (P =0.000).结论 bDNA法可定量检测TCT标本中HPV癌基因E6/E7 mRNA;HPV癌基因E6/E7 mRNA表达很可能促进宫颈病变的发生发展,对分流感染HPV及细胞学阳性患者有较好的临床应用前景.
目的 探討人乳頭瘤病毒(HPV)癌基因E6/E7 mRNA檢測在宮頸病變中的臨床意義.方法 收集鄭州大學第三附屬醫院行宮頸液基薄層細胞學(TCT)檢查,且經第二代雜交捕穫法(HC2)證實HPV DNA暘性的患者280例,採用QUANTIVIRUS(a) HPV E6/E7 mRNA3.0 Assay(bDNA)法檢測宮頸脫落細胞中HPV癌基因E6/E7 mRNA水平,併隨訪其組織病理結果.結果 280例患者的細胞學結果:未見上皮內病變或噁性細胞(NILM) 27例(9.64％),非典型鱗狀上皮細胞(ASC) 94例(33.57％),低度鱗狀上皮內病變(LSIE)70例(25.00％),高度鱗狀上皮內病變(HSIL) 67例(23.93％),鱗癌(SCC) 22例(7.86％).不同細胞學診斷級彆間HPV癌基因E6/E7 mRNA水平比較,差異具有統計學意義(F=2.827,P=0.025),病變組(ASC、LSIL、HSIL、SCC) HPV癌基因E6/E7 mRNA水平均顯著高于NILM組(*P=0.000、**P=0.000、***P=0.016、****P=0.022).隨宮頸細胞學病變程度加重,HPV癌基因E6/E7 mRNA錶達增高;280例患者有1 84例追蹤到組織學結果:慢性宮頸炎51例(27.72％),宮頸上皮內瘤樣病變Ⅰ級(CIN Ⅰ)46例(25.00％),宮頸上皮內瘤樣病變Ⅱ級(CINⅡ)25例(13.59％),宮頸上皮內瘤樣病變Ⅲ級(CINⅢ)30例(16.30％),SCC 32例(17.39％).不同組織學診斷級彆組間HPV癌基因E6/E7 mRNA水平比較,差異顯著(F=2.682,P=0.033),病變組(CIN Ⅰ、CINⅡ、CINⅢ、SCC) HPV癌基因E6/E7 mRNA水平均顯著高于慢炎組(*P=0.016、**P=0.025、***P=0.007、**** P=0.039).隨宮頸組織學病變程度加重,HPV癌基因E6/E7 mRNA錶達水平增高;將細胞學病變程度和組織學病變程度分彆與HPV癌基因E6/E7mRNA水平進行spearman等級相關分析,相關繫數r分彆為0.188(P=0.001)與0.582 (P =0.000).結論 bDNA法可定量檢測TCT標本中HPV癌基因E6/E7 mRNA;HPV癌基因E6/E7 mRNA錶達很可能促進宮頸病變的髮生髮展,對分流感染HPV及細胞學暘性患者有較好的臨床應用前景.
목적 탐토인유두류병독(HPV)암기인E6/E7 mRNA검측재궁경병변중적림상의의.방법 수집정주대학제삼부속의원행궁경액기박층세포학(TCT)검사,차경제이대잡교포획법(HC2)증실HPV DNA양성적환자280례,채용QUANTIVIRUS(a) HPV E6/E7 mRNA3.0 Assay(bDNA)법검측궁경탈락세포중HPV암기인E6/E7 mRNA수평,병수방기조직병리결과.결과 280례환자적세포학결과:미견상피내병변혹악성세포(NILM) 27례(9.64％),비전형린상상피세포(ASC) 94례(33.57％),저도린상상피내병변(LSIE)70례(25.00％),고도린상상피내병변(HSIL) 67례(23.93％),린암(SCC) 22례(7.86％).불동세포학진단급별간HPV암기인E6/E7 mRNA수평비교,차이구유통계학의의(F=2.827,P=0.025),병변조(ASC、LSIL、HSIL、SCC) HPV암기인E6/E7 mRNA수평균현저고우NILM조(*P=0.000、**P=0.000、***P=0.016、****P=0.022).수궁경세포학병변정도가중,HPV암기인E6/E7 mRNA표체증고;280례환자유1 84례추종도조직학결과:만성궁경염51례(27.72％),궁경상피내류양병변Ⅰ급(CIN Ⅰ)46례(25.00％),궁경상피내류양병변Ⅱ급(CINⅡ)25례(13.59％),궁경상피내류양병변Ⅲ급(CINⅢ)30례(16.30％),SCC 32례(17.39％).불동조직학진단급별조간HPV암기인E6/E7 mRNA수평비교,차이현저(F=2.682,P=0.033),병변조(CIN Ⅰ、CINⅡ、CINⅢ、SCC) HPV암기인E6/E7 mRNA수평균현저고우만염조(*P=0.016、**P=0.025、***P=0.007、**** P=0.039).수궁경조직학병변정도가중,HPV암기인E6/E7 mRNA표체수평증고;장세포학병변정도화조직학병변정도분별여HPV암기인E6/E7mRNA수평진행spearman등급상관분석,상관계수r분별위0.188(P=0.001)여0.582 (P =0.000).결론 bDNA법가정량검측TCT표본중HPV암기인E6/E7 mRNA;HPV암기인E6/E7 mRNA표체흔가능촉진궁경병변적발생발전,대분류감염HPV급세포학양성환자유교호적림상응용전경.
Objective To investigate the clinical significance of human papillomavirus (HPV) oncogene E6/E7 mRNA testing for cervical lesions.Methods The clinical materials consisted of 280 women,with positive HPV DNA testing,from the Third affiliated hospital of Zhengzhou University,during the period from January 2011 up to December 2011,were selected under the examinations of thinprep cytologic test (TCT) and hybrid capture Ⅱ (HC2) test.All TCT samples were studied for the level of HPV E6/E7 mRNA by QUANTIVIRUS（R） HPV E6/E7 mRNA 3.0 Assay (bDNA).Subjects with cervical biopsy were followwed up the histopathological results.Results The cytologic pathological results of the 280 cases were as follows:27 (9.64％) cases were negative for intraepithelial lesion(NILM),94 (33.57％)cases were atypical squamous cells (ASC),70(25.00％)cases were low-grade squamous intraepithelial lesion (LSIL),67 (23.93％)cases were high-grade squamous intraepithelial lesion (HSIL) and 22 (7.86％) cases were squamous cell carcinoma (SCC).The levels of HPV E6/E7 mRNA in abnormal cytologic result groups (include ASC,LSIL,HSIL and SCC) were significantly higher than that in NILM group respectively (* P =0.000,**P =0.000,***P =0.016,****P =0.022).HPV E6/E7 mRNA expression increased with severity of cervical cytologic lesions.One hundred eight four,of 280 cases,were traced the histologic results.According to the pathological diagnosis,51 (27.72％) cases were chronic cervicitis,46 (25.00％) cases were cervical intraepithelial neoplasia (CIN) Ⅰ,25 (13.59％) cases were CIN Ⅱ,30 (16.30％) cases were CIN Ⅲ and 32 (17.39％) cases were squamous cell carcinoma (SCC).The levels of HPV E6/E7 mRNA in abnormal pathological groups (include CIN Ⅰ,CIN Ⅱ,CIN Ⅲ and SCC) were significantly higer than that in chronic cervicitis group (*P=0.016,**P=0.025,***P =0.007,****P =0.039).HPV E6/E7 mRNA transcripts increased with severity of histologic lesions.The spearman rank assay was used to analyse the correlation between HPV E6/E7 mRNA expression with severity of cervical cytologic lesions (r=0.188,P=0.001) and histologic lesions(r =0.582,P=0.000).Conclusion TCT specimen are ideal to be used to detect HPV E6/E7 mRNA by bDNA.The up-regulation of HPV E6/E7 mRNA probably promotes the development and progression of cervical intraepithelial neoplasia.Testing for HPV E6/E7 transcripts may serve as a more specific discriminator between transient cervical dysplasias and potentially progressive lesions.
