CAJ | 학술논문

基因组不稳定是肿瘤细胞的特征性标志,基因扩增是一种特殊的基因组不稳定性的表现形式,其在激活原癌基因引发癌症的过程中起着重要的作用.基因扩增将导致细胞加速生长增殖或耐药生长等表型.人们已经广泛注意到其在癌症的诊断和治疗等方面的重要临床意义.但是,人们对基因扩增的分子机制的认识仍然十分有限,本文综述了回文序列介导的基因扩增的分子机制,提出在DNA断点处以无模板形式、新合成小回文序列可能是一部分肿瘤细胞中基因扩增的关键机制,为进一步探索基因扩增的分子机制提供理论参考.
기인조불은정시종류세포적특정성표지,기인확증시일충특수적기인조불은정성적표현형식,기재격활원암기인인발암증적과정중기착중요적작용.기인확증장도치세포가속생장증식혹내약생장등표형.인문이경엄범주의도기재암증적진단화치료등방면적중요림상의의.단시,인문대기인확증적분자궤제적인식잉연십분유한,본문종술료회문서렬개도적기인확증적분자궤제,제출재DNA단점처이무모판형식、신합성소회문서렬가능시일부분종류세포중기인확증적관건궤제,위진일보탐색기인확증적분자궤제제공이론삼고.
Gene amplification is a common chromosomal aberration which is often the result of tumor genome instability,and plays a crucial role in the process of activating oncogenes and leading to cancers.As gene amplification often is evident in advanced stages of cancer,its clinical importance in diagnosis and treatment has been popularly recognized.However,the underlying mechanisms governing gene amplification are still not fully understood.Herein,we reviewed one of the well-defined gene amplification mechanisms:DNA double-strand breaks-triggered,palindromes or short DNA inverted repeatsmediated gene amplification model,which was established and validated in a variety of research organisms including T.thermophila,fission yeast,budding yeast and rodent cells.Based on the recent published reports,we put forward a new model for the palindromes mediated gene amplification mechanism,that is de novo synthesis of small palindromic sequences (usually several hundred base pairs in length or shorter) at the DNA breakpoints in a template-free manner is the key determinant for gene amplification in certain tumor genomes.Elucidating the potential mechanism and involved enzymes for the regulation and creation of novel palindromic sequences should shed more light onto the palindromes mediated gene amplification and the resulting chromosomal instability.