CAJ | 학술논문

染色体步移技术是一种重要的分子生物学研究技术,该技术现阶段主要分为结合基因组文库的染色体步移技术和基于PCR扩增的染色体步移技术.鉴于染色体步移技术可以克隆已知序列相邻的侧翼序列,所以主要应用于克隆已知序列的侧翼启动子和调控原件、鉴定T-DNA转座子或转座子的插入位点、探知特定位点的上下游序列和染色体人工测序中的空隙填补,从而获得完整的基因组序列.随着染色体步移技术的不断改进,其在基因组学和分子生物学中得到了广泛的应用.该文就目前染色体步移技术的应用及进展加以综述.
염색체보이기술시일충중요적분자생물학연구기술,해기술현계단주요분위결합기인조문고적염색체보이기술화기우PCR확증적염색체보이기술.감우염색체보이기술가이극륭이지서렬상린적측익서렬,소이주요응용우극륭이지서렬적측익계동자화조공원건、감정T-DNA전좌자혹전좌자적삽입위점、탐지특정위점적상하유서렬화염색체인공측서중적공극전보,종이획득완정적기인조서렬.수착염색체보이기술적불단개진,기재기인조학화분자생물학중득도료엄범적응용.해문취목전염색체보이기술적응용급진전가이종술.
Chromosome walking technique is an important technique in molecular biology research.Chromosome walking technique by its means is divided into genomic library combined technology and PCR-based technology.As the chromosome walking technique may clone the flanking sequences adjacent to a known sequence,this technique is mainly used in cloning the promoter of known sequence and flanking regulatory element,identifying T-DNA transposon and insertion site of the transposon,discovering upstream and downstream sequence of specific genomic site,gap filling in artificial chromosome sequencing as to obtain the complete genome sequence.As chromosome walking technology continues to improve,it is widely used in genomics and molecular biology.In this review,we demonstrate some key applications of chromosome walking techniques in recent years.