国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2014年
3期
103-108
,共6页
贾熙卓%郑天虎%李艳泽%刁岩%高旭
賈熙卓%鄭天虎%李豔澤%刁巖%高旭
가희탁%정천호%리염택%조암%고욱
胶质瘤%阿托伐他汀%趋化因子受体4%趋化因子12
膠質瘤%阿託伐他汀%趨化因子受體4%趨化因子12
효질류%아탁벌타정%추화인자수체4%추화인자12
Glioma%Atorvastatin%CXCR4%CXCL12
目的 探讨阿托伐他汀(AVT)对人胶质瘤细胞U87侵袭性的抑制作用及其相关机制的研究.方法 采用Transwell法检测AVT抑制胶质瘤细胞侵袭的效率,RT-PCR和Western印迹方法检测AVT对CXCL12和CXCR4表达的影响.结果 RT-PCR实验结果表明AVT作用一定时间(24 h),随着AVT浓度增大,CXCR4 mRNA表达量逐渐减小.AVT浓度为1.0、2.0、8.0 μmol/L AVT与未加AVT对照组差异具有统计学意义(P值分别为0.045、0.021、0.002);在AVT浓度一定时(2.0 μmol/L AVT),AVT作用时间越长,CXCR4 mRNA表达量越小.AVT作用时间为12、24、36 h与未加AVT对照组差异具有统计学意义(P值分别为0.040、0.019、0.001).AVT对CXCL 12 mRNA表达影响不显著,与对照组差异无统计学意义(P值为0.869).结论 AVT能够显著抑制胶质瘤细胞U87的侵袭性,并且抑制趋化因子CXCR4的表达水平,AVT可能通过下调CXCR4的表达从而抑制胶质瘤细胞的侵袭性.
目的 探討阿託伐他汀(AVT)對人膠質瘤細胞U87侵襲性的抑製作用及其相關機製的研究.方法 採用Transwell法檢測AVT抑製膠質瘤細胞侵襲的效率,RT-PCR和Western印跡方法檢測AVT對CXCL12和CXCR4錶達的影響.結果 RT-PCR實驗結果錶明AVT作用一定時間(24 h),隨著AVT濃度增大,CXCR4 mRNA錶達量逐漸減小.AVT濃度為1.0、2.0、8.0 μmol/L AVT與未加AVT對照組差異具有統計學意義(P值分彆為0.045、0.021、0.002);在AVT濃度一定時(2.0 μmol/L AVT),AVT作用時間越長,CXCR4 mRNA錶達量越小.AVT作用時間為12、24、36 h與未加AVT對照組差異具有統計學意義(P值分彆為0.040、0.019、0.001).AVT對CXCL 12 mRNA錶達影響不顯著,與對照組差異無統計學意義(P值為0.869).結論 AVT能夠顯著抑製膠質瘤細胞U87的侵襲性,併且抑製趨化因子CXCR4的錶達水平,AVT可能通過下調CXCR4的錶達從而抑製膠質瘤細胞的侵襲性.
목적 탐토아탁벌타정(AVT)대인효질류세포U87침습성적억제작용급기상관궤제적연구.방법 채용Transwell법검측AVT억제효질류세포침습적효솔,RT-PCR화Western인적방법검측AVT대CXCL12화CXCR4표체적영향.결과 RT-PCR실험결과표명AVT작용일정시간(24 h),수착AVT농도증대,CXCR4 mRNA표체량축점감소.AVT농도위1.0、2.0、8.0 μmol/L AVT여미가AVT대조조차이구유통계학의의(P치분별위0.045、0.021、0.002);재AVT농도일정시(2.0 μmol/L AVT),AVT작용시간월장,CXCR4 mRNA표체량월소.AVT작용시간위12、24、36 h여미가AVT대조조차이구유통계학의의(P치분별위0.040、0.019、0.001).AVT대CXCL 12 mRNA표체영향불현저,여대조조차이무통계학의의(P치위0.869).결론 AVT능구현저억제효질류세포U87적침습성,병차억제추화인자CXCR4적표체수평,AVT가능통과하조CXCR4적표체종이억제효질류세포적침습성.
Objective To explore the mechanism of atorvastatin (AVT) on inhibition of invasion in glioma U87 cells.Methods To detect the efficiency of AVT on inhibition of the invassion of glioma cells by using Transwell kit,RT-PCR and Western blot methods were used to study the effects of AVT on CXCL12 and CXCR4 expression.Results The results showed that the effect of atorvastatin (AVT),after 24 hour treatment,is an dose-dependent decrease in CXCR4mRNA expression with the increasing concentration of atorvastatin.Compared with control group,the cells treated with 1.0,2.0,8.0 μmol/ L AVT have showed statistically significant changes (P values are 0.045,0.021,0.002 respectively).At the same concentration of AVT (2.0 μmol/ L),the expression level of CXCR4 mRNA decreased,following a time-dpendent manner (12,24,36 hours),and being statistically significant (P values are 0.040,0.019,0.001 respectively).The AVT administration does not have effects on the expression of CXCL12 mRNA,statistically,with P value being 0.869.Conclusion AVT significantly inhibited the invasion of glioma U87 cells clinically,and inhibited the expression of CXCR4 experimentally.Therefore we speculate that the AVT maybe inhibit glioma cell invasiveness through downregulation of CXCR4 expression.
