CAJ | 학술논문

目的观察新生大鼠缺氧缺血性脑损伤(HIBD)脑组织Bcl-2、Bax基因表达变化及氨基甲酰化促红细胞生成素(CEPO)干预对其表达的影响,探讨CEPO发挥脑保护作用的可能机制.方法将新生7日龄SD大鼠建立HIBD模型,利用RT-PCR检测缺氧缺血和CEPO干预后2h、12h、24 h、48 h、72 h凋亡基因Bcl-2、Bax的mRNA表达的改变.结果与假手术组相比,缺氧缺组在2h、12h、24 h、48 h、72 h脑组织中Bcl-2、Bax的表达均增加(P＜0.05),与缺氧缺血组相比,CEPO干预组在不同时间点Bcl-2表达增加(P＜0.05),Bax表达下降(P＜0.05).结论在新生大鼠HIBD中Bcl-2、Bax mRNA的表达发生改变,调控二者的表达水平可能是CEPO发挥脑保护的作用机制之一.
목적 관찰신생대서결양결혈성뇌손상(HIBD)뇌조직Bcl-2、Bax기인표체변화급안기갑선화촉홍세포생성소(CEPO)간예대기표체적영향,탐토CEPO발휘뇌보호작용적가능궤제.방법 장신생7일령SD대서건립HIBD모형,이용RT-PCR검측결양결혈화CEPO간예후2h、12h、24 h、48 h、72 h조망기인Bcl-2、Bax적mRNA표체적개변.결과 여가수술조상비,결양결조재2h、12h、24 h、48 h、72 h뇌조직중Bcl-2、Bax적표체균증가(P＜0.05),여결양결혈조상비,CEPO간예조재불동시간점Bcl-2표체증가(P＜0.05),Bax표체하강(P＜0.05).결론 재신생대서HIBD중Bcl-2、Bax mRNA적표체발생개변,조공이자적표체수평가능시CEPO발휘뇌보호적작용궤제지일.
Objective To investigated the association between the expressions of Bcl-2 and Bax genes in neonatal rats upon cerebral hypoxic-ischemia and the effects of Carbamylated Erythropoietin (CEPO.) administration on both genes.Methods An animal model of neonatal hypoxic-ischemia brain damage was established.The changes of Bcl-2 and Bax mRNAs in brain tissues were detected in rats after HI or CEPO administration by RT-PCR.Results The levels of Bcl-2 and Bax mRNAs were increased upon HIBD compared with sham-operated group at 2 h,12 h,24 h,48 h,72 h time points post treatment (P ＜ 0.05).On the contrary,only the level of Bcl-2 mRNA was increased (P ＜ 0.05) while the level of Bax mRNA was decreased (P ＜ 0.05) in CEPOintervention group.Conclusion The levels of Bcl-2 and Bax mRNAs in HIBD of neonatal rats were altered.CEPO may exert some neuroprotective effects on the brain tissues via interventing the expression of Bcl-2 and Bax.