国际遗传学杂志
國際遺傳學雜誌
국제유전학잡지
INTERNATIONAL JOURNAL OF GENETICS
2014年
5期
224-229
,共6页
恶性纤维组织细胞瘤%microRNA%生物信息学%差异表达%调控网络
噁性纖維組織細胞瘤%microRNA%生物信息學%差異錶達%調控網絡
악성섬유조직세포류%microRNA%생물신식학%차이표체%조공망락
Malignant fibrous histiocytoma%microRNA%Bioinformatics%Differential expression%Regulated network
目的 应用芯片表达分析技术探讨miRNA在恶性纤维组织细胞瘤发病机制中的作用.方法 利用miRCURYTM LNA miRNA表达谱芯片技术高通量筛选NMFH1与MSC、HSF、MRC-5和HT-1080细胞系差异表达miRNA,筛选靶基因,分析调控作用机制.结果 筛查NMFH1与4种对照组细胞系差异表达miRNA,选取与所有正常样本对比均下调miR-222、miR-186及miR-933,均上调miR-886-3p、miR-886-5p、miR-183、miR-340及miR-BART7进行qRT-PCR实验,验证芯片数据可靠性.基于生物信息学方法挖掘miRNA靶基因,构建基因调控网络,基于功能组注释信息分析NMFH1风险疾病基因及通路,揭示MFH恶性病变机制.结论 miRNA在肿瘤样本与正常细胞系间显著差异表达,是参与MFH发生发展过程的重要调控因子,发挥负调控基因表达作用,使肿瘤细胞逃避正常生长调控机制,无限增殖和转移,出现恶性表型.
目的 應用芯片錶達分析技術探討miRNA在噁性纖維組織細胞瘤髮病機製中的作用.方法 利用miRCURYTM LNA miRNA錶達譜芯片技術高通量篩選NMFH1與MSC、HSF、MRC-5和HT-1080細胞繫差異錶達miRNA,篩選靶基因,分析調控作用機製.結果 篩查NMFH1與4種對照組細胞繫差異錶達miRNA,選取與所有正常樣本對比均下調miR-222、miR-186及miR-933,均上調miR-886-3p、miR-886-5p、miR-183、miR-340及miR-BART7進行qRT-PCR實驗,驗證芯片數據可靠性.基于生物信息學方法挖掘miRNA靶基因,構建基因調控網絡,基于功能組註釋信息分析NMFH1風險疾病基因及通路,揭示MFH噁性病變機製.結論 miRNA在腫瘤樣本與正常細胞繫間顯著差異錶達,是參與MFH髮生髮展過程的重要調控因子,髮揮負調控基因錶達作用,使腫瘤細胞逃避正常生長調控機製,無限增殖和轉移,齣現噁性錶型.
목적 응용심편표체분석기술탐토miRNA재악성섬유조직세포류발병궤제중적작용.방법 이용miRCURYTM LNA miRNA표체보심편기술고통량사선NMFH1여MSC、HSF、MRC-5화HT-1080세포계차이표체miRNA,사선파기인,분석조공작용궤제.결과 사사NMFH1여4충대조조세포계차이표체miRNA,선취여소유정상양본대비균하조miR-222、miR-186급miR-933,균상조miR-886-3p、miR-886-5p、miR-183、miR-340급miR-BART7진행qRT-PCR실험,험증심편수거가고성.기우생물신식학방법알굴miRNA파기인,구건기인조공망락,기우공능조주석신식분석NMFH1풍험질병기인급통로,게시MFH악성병변궤제.결론 miRNA재종류양본여정상세포계간현저차이표체,시삼여MFH발생발전과정적중요조공인자,발휘부조공기인표체작용,사종류세포도피정상생장조공궤제,무한증식화전이,출현악성표형.
Objective To investigate the differential expression of microRNA in MFH by miRNA array technique.Methods A high throughput MiRCURYTM LNA miRNA array was applied to identify the microRNAs with differential expression in the novel MFH cell (NMFH) in comparison with 4 control cell lines including·embryonic lung fibroblast (MRC-5) 、mesenchymal stem cell (MSC) 、human skin fibrous (HSF) and fibrosarcoma (HT-1080) cell.Results Three down-regulated miR-222,miR-186,miR-933 and five up-regulated miR-886-3p,miR-886-5p,miR-183,miR-340,miR-BART7 were picked for qRT-PCR validation based on their expression level changes and their similar status in the 4 control cell lines.Potential target genes of these miRNA,their regulated networks,the disease associated genes and pathways were analyzed by bioinformatic methods in combination with functional annotation.Conclusion Differential expression of miRNAs in the tumor cells,is an important regulatory factor in the occurrence and development of MFH.It makes the tumor cells escape the normal growth regulation,and become malignant with capacity of unlimited proliferation and metastasis.
