CAJ | 학술논문

目的蛋白质相互作用研究对于理解DNA功能、基因组元件作用机制有重要意义.已有大量工作在从蛋白质-蛋白质互作的计算预测方向展开,其中蛋白质-蛋白质互作的特征起着很重要的作用.互作特征的评价及相关数据库构建仍是蛋白质互作预测的一项重要工作.方法该文从基因、蛋白质序列、功能信息层面提取出人类蛋白质-蛋白质对的27个特征,并应用于多种分类器,利用ROC曲线对特征的性能给出了科学的评价.结果通过研究发现逻辑回归和贝叶斯网络分类效果最好,生物过程、细胞组分、分子功能、基因表达值、组织、域间互作的可用性明显高于其他特征,同时构建了人类蛋白质互作特征数据库,供广大科研工作者使用.结论从多角度评价了特征可用性,得到了表现较优的特征,但是对于其中一些特征,还需要进一步提高覆盖率,从而达到更好的效果.
목적 단백질상호작용연구대우리해DNA공능、기인조원건작용궤제유중요의의.이유대량공작재종단백질-단백질호작적계산예측방향전개,기중단백질-단백질호작적특정기착흔중요적작용.호작특정적평개급상관수거고구건잉시단백질호작예측적일항중요공작.방법 해문종기인、단백질서렬、공능신식층면제취출인류단백질-단백질대적27개특정,병응용우다충분류기,이용ROC곡선대특정적성능급출료과학적평개.결과 통과연구발현라집회귀화패협사망락분류효과최호,생물과정、세포조분、분자공능、기인표체치、조직、역간호작적가용성명현고우기타특정,동시구건료인류단백질호작특정수거고,공엄대과연공작자사용.결론 종다각도평개료특정가용성,득도료표현교우적특정,단시대우기중일사특정,환수요진일보제고복개솔,종이체도경호적효과.
Objective Protein-protein interaction (PPI) studies are important for understanding the DNA function andfunctional mechanism of genomic elements.A lot of works had been done towards the direction of calculating predictions of PPIs,and these methods are a very important tool for determining the PPI chaiacteristics.Still much more works remain to be done for evaluating the PPI characteristics and building a related database.Methods we extracted 27 PPI features from gene,protein sequences and functional information in human,then apphed them to various classifiers and evaluated the performance of all the classifiers and features by ROC curve.Results Through our analysis,we found that logistic regression and bayesian network classification are best for PPI characteristics.Biological Process,Cell Composition,Molecular Function,Gene Expression Values,Organization,and Availability of interactions between domain were obviously more useful than other characteristics.Meanwhile,we built a easy using Human Protein Feature Database (HPFD).Conclusion We discovered PPI characteristics with better performance usability in evaluation of function characteristic.However,some of these characteristics,still need to be further optimized in terms of improved PPI coverage.