CAJ | 학술논문

目的制备13价肺炎链球菌多糖结合疫苗,并初步研究其免疫原性.方法将通过偶联方法制成的13种单价肺炎链球菌多糖结合疫苗,配制成含或不含铝佐剂的13价肺炎链球菌多糖结合疫苗.用制备的2种疫苗分别免疫猕猴和家兔,以ELISA法检测免疫动物的血清抗多糖抗体水平.结果制备的2种疫苗的各项指标均达到质控标准.末次免疫后,含或不含铝佐剂疫苗免疫猕猴的平均血清抗各型多糖抗体阳转率分别为98.08％和94.23％；含或不含铝佐剂疫苗免疫家兔的平均血清抗各型多糖抗体阳转率分别为95.38％和96.92％.结论成功研制了13价肺炎链球菌多糖结合疫苗,含或不含铝佐剂疫苗在猕猴和家兔中均具有免疫原性.
목적 제비13개폐염련구균다당결합역묘,병초보연구기면역원성.방법 장통과우련방법제성적13충단개폐염련구균다당결합역묘,배제성함혹불함려좌제적13개폐염련구균다당결합역묘.용제비적2충역묘분별면역미후화가토,이ELISA법검측면역동물적혈청항다당항체수평.결과 제비적2충역묘적각항지표균체도질공표준.말차면역후,함혹불함려좌제역묘면역미후적평균혈청항각형다당항체양전솔분별위98.08％화94.23％；함혹불함려좌제역묘면역가토적평균혈청항각형다당항체양전솔분별위95.38％화96.92％.결론 성공연제료13개폐염련구균다당결합역묘,함혹불함려좌제역묘재미후화가토중균구유면역원성.
Objective To prepare 13-valent pneumococcal polysaccharide conjugate vaccines and study their immunogenicity preliminarily.Methods 13 monovalent pneumococcal polysaccharide conjugate vaccines were prepared by coupling method.13-valent pneumococcal polysaccharide conjugate vaccines with or without aluminium adjuvant were prepared by mixing 13 monovalent pneumococcal polysaccharide conjugate vaccines.Macaques and rabbits were immunized with both kinds of prepared vaccines,and levels of serum antibodies to polysaccharide were detected by ELISA.Results All the indexes of two kinds of prepared vaccines met the standard of quality control.After the last immunization,average seroconversion rates in macaques immunized with 13-valent pneumococcal polysaccharide conjugate vaccine with or without aluminium adjuvant were 98.08％ and 94.23％,respectively; average seroconversion rates in rabbits immunized with 13-valent pneumococcal polysaccharide conjugate vaccine with or without aluminium adjuvant were 95.38％ and 96.92％,respectively.Conclusions 13-valent pneumococcal polysaccharide conjugate vaccines are successfully prepared.No matter whether the vaccines contain adjuvant or not,they have immunogenicity in macaques and rabbits.