国际生物制品学杂志
國際生物製品學雜誌
국제생물제품학잡지
INTERNATIONAL JOURNAL OF BIOLOGICALS
2013年
5期
225-228
,共4页
肝炎病毒,乙型%肝炎表面抗原,乙型%酶联免疫吸附测定
肝炎病毒,乙型%肝炎錶麵抗原,乙型%酶聯免疫吸附測定
간염병독,을형%간염표면항원,을형%매련면역흡부측정
Hepatitis B virus%Hepatitis B surface antigens%Enzyme-linked immunosorbent assay
目的 建立小鼠血清乙型肝炎病毒(hepatitis B virus,HBV)前S1抗体的间接ELISA检测方法.方法 以合成的HBV前S1(21-47位氨基酸)多肽包被酶标板,利用方阵滴定法确定间接ELISA方法的最适工作条件,对该法的精密度、特异性和灵敏度进行验证,并与商品化试剂盒进行比较.结果 确定最佳抗原包被浓度为1.0 mg/L,血清稀释度为1∶10.该方法的特异性、灵敏度和精密度均符合检测要求.本法与商品化前S1抗体检测试剂盒测定结果的符合率为98.0％.结论 成功建立了HBV前S1抗体间接ELISA方法,可用于小鼠血清中前S1抗体的检测.
目的 建立小鼠血清乙型肝炎病毒(hepatitis B virus,HBV)前S1抗體的間接ELISA檢測方法.方法 以閤成的HBV前S1(21-47位氨基痠)多肽包被酶標闆,利用方陣滴定法確定間接ELISA方法的最適工作條件,對該法的精密度、特異性和靈敏度進行驗證,併與商品化試劑盒進行比較.結果 確定最佳抗原包被濃度為1.0 mg/L,血清稀釋度為1∶10.該方法的特異性、靈敏度和精密度均符閤檢測要求.本法與商品化前S1抗體檢測試劑盒測定結果的符閤率為98.0％.結論 成功建立瞭HBV前S1抗體間接ELISA方法,可用于小鼠血清中前S1抗體的檢測.
목적 건립소서혈청을형간염병독(hepatitis B virus,HBV)전S1항체적간접ELISA검측방법.방법 이합성적HBV전S1(21-47위안기산)다태포피매표판,이용방진적정법학정간접ELISA방법적최괄공작조건,대해법적정밀도、특이성화령민도진행험증,병여상품화시제합진행비교.결과 학정최가항원포피농도위1.0 mg/L,혈청희석도위1∶10.해방법적특이성、령민도화정밀도균부합검측요구.본법여상품화전S1항체검측시제합측정결과적부합솔위98.0％.결론 성공건립료HBV전S1항체간접ELISA방법,가용우소서혈청중전S1항체적검측.
Objective To develop an indirect enzyme-linked immunosorbent assay (ELISA) for detection of hepatitis B virus (HBV) PreS1 antibody in mouse serum.Methods Microtiter wells were coated with synthesized HBV PreS1 polypeptides (21-47amino acid) and optimal reaction conditions were determined by criss-cross serial dilution analysis.Validation tests were performed to ensure feasibility of the developed method.Specificity,sensitivity and precision of the method were compared with those of a commercial kit.Results The optimal antigen concentration for coating was 1.0 mg/L and the dilution of serum was 1∶ 10.The specificity,sensitivity,and precision of the method were feasible for quality tests of vaccines.The overall coincidence rate of results by the developed method and the commercial kit was 98.0％ in 150 mouse sera.Conclusion The indirect ELISA suitable for detection of HBV PreS1 antibody in mouse serum is well developed and validated.
