CAJ | 학술논문

目的建立白喉杆菌培养基的相关质控标准.方法分别制备氨氮含量≥1.8 g/L和＜1.8 g/L的白喉杆菌培养基用于白喉杆菌大罐培养,采用t检验对培养结果进行统计学分析,确定最佳培养基配方.按确定的培养基配方制备15批白喉杆菌培养基用于白喉杆菌大罐培养,检测培养收获的白喉毒素絮状单位,并分析培养基氨氮含量与白喉杆菌产毒间的相关关系.配制15批15％～20％的麦芽糖溶液,分别作为补料于白喉杆菌培养过程中加入大罐,检测培养收获的白喉毒素絮状单位,并分析麦芽糖溶液浓度与白喉杆菌产毒间的相关关系.结果采用氨氮含量≥1.8 g/L的培养基培养收获的白喉毒素絮状单位明显高于采用氨氮含量＜1.8 g/L的培养基培养(t=0.5635,P＜0.05).在确定的氨氮含量范围(1.8 ～2.0 g/L)内,培养基氨氮含量与收获的白喉毒素絮状单位呈线性正相关(r=0.52).当麦芽糖溶液浓度为15％～ 20％时,麦芽糖溶液浓度与收获的白喉毒素絮状单位呈线性负相关(r=-0.53).结论白喉杆菌培养基氨氮含量控制在1.8 ～2.0 g/L,补料麦芽糖溶液浓度控制在15％～20％时,大罐培养收获的白喉毒素絮状单位可达到规程要求.
목적 건립백후간균배양기적상관질공표준.방법 분별제비안담함량≥1.8 g/L화＜1.8 g/L적백후간균배양기용우백후간균대관배양,채용t검험대배양결과진행통계학분석,학정최가배양기배방.안학정적배양기배방제비15비백후간균배양기용우백후간균대관배양,검측배양수획적백후독소서상단위,병분석배양기안담함량여백후간균산독간적상관관계.배제15비15％～20％적맥아당용액,분별작위보료우백후간균배양과정중가입대관,검측배양수획적백후독소서상단위,병분석맥아당용액농도여백후간균산독간적상관관계.결과 채용안담함량≥1.8 g/L적배양기배양수획적백후독소서상단위명현고우채용안담함량＜1.8 g/L적배양기배양(t=0.5635,P＜0.05).재학정적안담함량범위(1.8 ～2.0 g/L)내,배양기안담함량여수획적백후독소서상단위정선성정상관(r=0.52).당맥아당용액농도위15％～ 20％시,맥아당용액농도여수획적백후독소서상단위정선성부상관(r=-0.53).결론 백후간균배양기안담함량공제재1.8 ～2.0 g/L,보료맥아당용액농도공제재15％～20％시,대관배양수획적백후독소서상단위가체도규정요구.
Objective To establish relative quality standards of Corynebacterium diphtheriae culture medium.Methods Corynebacterium diphtheriae culture media with ≥ 1.8 g/L and ＜ 1.8 g/L ammonia nitrogen were prepared respectively to use for Corynebacterium diphtheriae culture in large fermtor.The statistical analysis of the culture results were made by t test,and the optimal culture medium was identified.Fifteen batches of Corynebacterium diphtheriae culture media were prepared according to the identified formula and used to large fermentor culture.Quantities of diphtheria toxin harvested by culture were detected,and correlativity between ammonia nitrogen contents in Corynebacterium diphtheriae culture medium and diphtheria toxin produced by Corynebacterium diphtheriae was analysed.Fifteen batches of 15％-20％ maltose solutions were made up and fed into large fermentor as supplement during Corynebacterium diphtheriae culture.Quantities of diphtheria toxin harvested by culture were detected,and correlativity between concentrations of maltose solutions and diphtheria toxin produced by Corynebacterium diphtheriae was analysed.Results Quantities of diphtheria toxin harvested from Corynebacterium diphtheriae culture media with ≥ 1.8 g/L ammonia nitrogen were significantly higher than those harvested from Corynebacterium diphtheriae culture media with ＜ 1.8 g/L ammonia nitrogen(t =0.5635,P ＜ 0.05).Positive linear correlation (r =0.52) was showed between ammonia nitrogen contents in Corynebacterium diphtheriae culture medium and quantities of diphtheria toxin in ranges of identified ammonia nitrogen contents (1.8-2.0 g/L).When concentrations of maltose solutions ranged from 15％ to 20％,negative linear correlation (r =-0.53) was showed between concentrations of maltose solutions and quantities of diphtheria toxin.Conclusion When ammonia nitrogen contents in Corynebacterium diphtheriae culture medium and concentrations of maltose solutions for feeding into large fermentor are controlled in ranges of 1.8-2.0 g/L and 15％-20％,respectively,quantities of diphtheria toxin harvested by large fermentor culture can achieve requirements of the regulation.