国际生物制品学杂志
國際生物製品學雜誌
국제생물제품학잡지
INTERNATIONAL JOURNAL OF BIOLOGICALS
2014年
2期
64-66
,共3页
龚曼琳%江砚芳%杨汇川%许欣
龔曼琳%江硯芳%楊彙川%許訢
공만림%강연방%양회천%허흔
白蛋白类%氢氧化钠%辅药%色谱法
白蛋白類%氫氧化鈉%輔藥%色譜法
백단백류%경양화납%보약%색보법
Albumins%Sodium hydroxide%Exipients%Chromatography
目的 初探不同碱处理条件对人血白蛋白(human serum albumin,HSA)分子的影响.方法 在保持pH 10.0±0.2的条件下,以不同钠离子浓度和辛酸钠浓度处理白蛋白,通过圆二色谱和高效液相色谱对碱处理的HSA进行检测,分析不同处理条件对HSA分子的影响.结果 在保持pH 10.0 ±0.2且辛酸钠浓度为0.16 mmol/g的条件下,以不同钠离子浓度(40～ 800 mmol/L)处理获得的单体和双体HSA含量均＞96％,而且碱处理HSA的圆二色谱峰值(6.7 ～8.2 cm)与合格HSA(7.5 ～8.9 cm)相近,峰值位置没有偏移.在保持pH 10.0±0.2且钠离子浓度为40 mmol/L的条件下,以不同辛酸钠浓度(0.16～ 1.00 mmol/g)处理获得的的单体和双体HSA含量均＞97％,碱处理HSA的圆二色谱峰值(6.1 ～8.2 cm)与合格HSA(7.5 ～ 8.9 cm)相近,峰值位置没有偏移.结论 在一定条件下对HSA进行碱处理不会对其结构产生明显影响.
目的 初探不同堿處理條件對人血白蛋白(human serum albumin,HSA)分子的影響.方法 在保持pH 10.0±0.2的條件下,以不同鈉離子濃度和辛痠鈉濃度處理白蛋白,通過圓二色譜和高效液相色譜對堿處理的HSA進行檢測,分析不同處理條件對HSA分子的影響.結果 在保持pH 10.0 ±0.2且辛痠鈉濃度為0.16 mmol/g的條件下,以不同鈉離子濃度(40～ 800 mmol/L)處理穫得的單體和雙體HSA含量均＞96％,而且堿處理HSA的圓二色譜峰值(6.7 ～8.2 cm)與閤格HSA(7.5 ～8.9 cm)相近,峰值位置沒有偏移.在保持pH 10.0±0.2且鈉離子濃度為40 mmol/L的條件下,以不同辛痠鈉濃度(0.16～ 1.00 mmol/g)處理穫得的的單體和雙體HSA含量均＞97％,堿處理HSA的圓二色譜峰值(6.1 ～8.2 cm)與閤格HSA(7.5 ～ 8.9 cm)相近,峰值位置沒有偏移.結論 在一定條件下對HSA進行堿處理不會對其結構產生明顯影響.
목적 초탐불동감처리조건대인혈백단백(human serum albumin,HSA)분자적영향.방법 재보지pH 10.0±0.2적조건하,이불동납리자농도화신산납농도처리백단백,통과원이색보화고효액상색보대감처리적HSA진행검측,분석불동처리조건대HSA분자적영향.결과 재보지pH 10.0 ±0.2차신산납농도위0.16 mmol/g적조건하,이불동납리자농도(40～ 800 mmol/L)처리획득적단체화쌍체HSA함량균＞96％,이차감처리HSA적원이색보봉치(6.7 ～8.2 cm)여합격HSA(7.5 ～8.9 cm)상근,봉치위치몰유편이.재보지pH 10.0±0.2차납리자농도위40 mmol/L적조건하,이불동신산납농도(0.16～ 1.00 mmol/g)처리획득적적단체화쌍체HSA함량균＞97％,감처리HSA적원이색보봉치(6.1 ～8.2 cm)여합격HSA(7.5 ～ 8.9 cm)상근,봉치위치몰유편이.결론 재일정조건하대HSA진행감처리불회대기결구산생명현영향.
Objective To preliminarily study the influence of different alkali treatment conditions on human serum albumin (HSA) molecules.Methods HSAs were treated with different sodium ion concentrations and different sodium caprylate concentrations at pH 10.0 ± 0.2.Alkali treated HSA was detected by circular dichroism and high performance liquid chromatography to analyse the influence of different treatment conditions on HSA molecules.Results Under the condition of pH 10.0 ± 0.2 and 0.16 mmol/g sodium caprylate,contents of monomer and dimer HSAs obtained by treatment of different sodium ion concentrations (40-800 mmol/L) were all ＞96％,and peak value of circular dichroism of alkali treated HSA (6.7-8.2 cm) was similar to that of the qualified HSA (7.5-8.9 cm) and peak value position did not migrate.Under the condition of pH 10.0 ± 0.2 and 40 mmol/L sodium ion,contents of monomer and dimer HSAs obtained by treatment of different sodium caprylate concentrations (0.16-1.00 mmol/g) were all ＞97％,and peak value of circular dichroism of alkali treated HSA (6.1-8.2 cm) was similar to that of the qualified HSA (7.5-8.9 cm) and peak value position did not migrate.Conclusion HSA structure does not fundamentally change when alkali treatment for HSA is carried out under certain conditions.
