国际生物制品学杂志
國際生物製品學雜誌
국제생물제품학잡지
INTERNATIONAL JOURNAL OF BIOLOGICALS
2014年
2期
67-72
,共6页
卞炯%祝慈芳%何毅明%朱威
卞炯%祝慈芳%何毅明%硃威
변형%축자방%하의명%주위
载脂蛋白AⅠ%工艺学,制药%纯化
載脂蛋白AⅠ%工藝學,製藥%純化
재지단백AⅠ%공예학,제약%순화
Apolipoprotein A-Ⅰ%Technology,pharmaceutical%Purification
目的 初探大规模纯化人血浆载脂蛋白AⅠ(apolipoprotein A Ⅰ,ApoA Ⅰ)的低成本生产工艺.方法 以人血浆组分Ⅳ为原料,通过等电点沉淀、阴离子层析、低温乙醇沉淀和超滤等步骤纯化ApoA Ⅰ蛋白,并放大实验室纯化方法来纯化ApoA Ⅰ.结果 放大纯化方法生产的ApoA Ⅰ的得率和纯度分别为59.0％和85.6％,经相关方法验证为人血浆ApoAⅠ.结论 实验室方法放大的纯化工艺可纯化ApoA Ⅰ.
目的 初探大規模純化人血漿載脂蛋白AⅠ(apolipoprotein A Ⅰ,ApoA Ⅰ)的低成本生產工藝.方法 以人血漿組分Ⅳ為原料,通過等電點沉澱、陰離子層析、低溫乙醇沉澱和超濾等步驟純化ApoA Ⅰ蛋白,併放大實驗室純化方法來純化ApoA Ⅰ.結果 放大純化方法生產的ApoA Ⅰ的得率和純度分彆為59.0％和85.6％,經相關方法驗證為人血漿ApoAⅠ.結論 實驗室方法放大的純化工藝可純化ApoA Ⅰ.
목적 초탐대규모순화인혈장재지단백AⅠ(apolipoprotein A Ⅰ,ApoA Ⅰ)적저성본생산공예.방법 이인혈장조분Ⅳ위원료,통과등전점침정、음리자층석、저온을순침정화초려등보취순화ApoA Ⅰ단백,병방대실험실순화방법래순화ApoA Ⅰ.결과 방대순화방법생산적ApoA Ⅰ적득솔화순도분별위59.0％화85.6％,경상관방법험증위인혈장ApoAⅠ.결론 실험실방법방대적순화공예가순화ApoA Ⅰ.
Objective To preliminarily explore a low-cost production process of purifying apolipoprotein A Ⅰ (ApoA Ⅰ) from human plasma in large scale.Methods ApoA Ⅰ was purified from human plasma fraction Ⅳ through isoelectric precipitation,anion exchange chromatography,ethanol precipitation and ultrafiltration steps,and the laboratory purification method was amplified to purify ApoA Ⅰ.Results The yield and purity of ApoA Ⅰ produced by amplified purification method were 59.0％ and 85.6％ respectively,and ApoA Ⅰ was verified with relative methods.Conclusion The purification process amplified from laboratory method can purify ApoA Ⅰ.
