国际生物制品学杂志
國際生物製品學雜誌
국제생물제품학잡지
INTERNATIONAL JOURNAL OF BIOLOGICALS
2014年
5期
217-219
,共3页
日本脑炎疫苗%脑炎病毒,日本%毒力%恒河猴
日本腦炎疫苗%腦炎病毒,日本%毒力%恆河猴
일본뇌염역묘%뇌염병독,일본%독력%항하후
Japanese encephalitis vaccines%Encephalitis virus,Japanese%Virulence%Macaca mulatta
目的 为了解乙型脑炎(乙脑)减毒活疫苗主种子批弱毒株的神经毒力减弱程度,对其进行猴体神经毒力试验,以观察其安全性.方法 对恒河猴脑内和脊髓内同时注射乙脑病毒强毒株SA14或减毒活疫苗主种子批弱毒株SA14-14-2,然后进行为期21 d的临床体征观察,并进行组织病理学检查.结果 强毒株组动物接种后的临床体征为典型的神经毒性反应,动物发病并死亡.组织病理学检查显示,中枢神经系统严重受损,并有神经细胞坏死.弱毒株组动物仅出现短时发热,组织病理学检查可见轻微炎症反应,未见神经细胞坏死现象.结论 乙脑减毒活疫苗主种子批的神经毒力高度减弱,对恒河猴是安全的.
目的 為瞭解乙型腦炎(乙腦)減毒活疫苗主種子批弱毒株的神經毒力減弱程度,對其進行猴體神經毒力試驗,以觀察其安全性.方法 對恆河猴腦內和脊髓內同時註射乙腦病毒彊毒株SA14或減毒活疫苗主種子批弱毒株SA14-14-2,然後進行為期21 d的臨床體徵觀察,併進行組織病理學檢查.結果 彊毒株組動物接種後的臨床體徵為典型的神經毒性反應,動物髮病併死亡.組織病理學檢查顯示,中樞神經繫統嚴重受損,併有神經細胞壞死.弱毒株組動物僅齣現短時髮熱,組織病理學檢查可見輕微炎癥反應,未見神經細胞壞死現象.結論 乙腦減毒活疫苗主種子批的神經毒力高度減弱,對恆河猴是安全的.
목적 위료해을형뇌염(을뇌)감독활역묘주충자비약독주적신경독력감약정도,대기진행후체신경독력시험,이관찰기안전성.방법 대항하후뇌내화척수내동시주사을뇌병독강독주SA14혹감독활역묘주충자비약독주SA14-14-2,연후진행위기21 d적림상체정관찰,병진행조직병이학검사.결과 강독주조동물접충후적림상체정위전형적신경독성반응,동물발병병사망.조직병이학검사현시,중추신경계통엄중수손,병유신경세포배사.약독주조동물부출현단시발열,조직병이학검사가견경미염증반응,미견신경세포배사현상.결론 을뇌감독활역묘주충자비적신경독력고도감약,대항하후시안전적.
Objective To evaluate safety of an attenuated Japanese encephalitis virus strain SA14-14-2 (master seed lot) in rhesus by a neurovirulence test.Methods Animals were inoculated with an virulent strain SA14 or the attenuated strain SA14-14-2 of Japanese encephalitis virus by intracranial and intraspinal routes.Clinical signs were observed for 21 days and histopathological examination was performed.Results The rhesuses injected with strain SA14 developed neurotoxic reactions and died.The histopathological results showed severe damage with neuronal cell necrosis in their central nervous systems.Only transient fever and mild inflammatory reaction were observed,and no neuronal cell necrosis was found in the animals receiving the attenuated SA14-14-2 virus.Conclusion The neurovirulence of strain SA14-14-2 is remarkably attenuated and the strain is safe in rhesus.
