CAJ | 학술논문

目的探讨溃疡性结肠炎(ulcerative colitis,UC)中半胱天冬氨酸酶-3(Caspase-3)、B细胞淋巴瘤/白血病基因2相关X蛋白(Bax)的表达水平与辨证分型的相关性.方法将UC患者辨证分为湿热内蕴型组(25例)、脾胃虚弱型组(25例),用免疫组织化学技术(SP法),检测两组患者结肠黏膜腺上皮细胞Caspase-3、Bax的表达水平情况；并与正常对照组(25例)进行比较.结果 Bax在湿热内蕴型[(4.56±1.58)分、脾胃虚弱型(3.28±1.14)分]的表达均升高,与正常对照组比较,差异均有统计学意义(P均＜0.0.5)；两型的Caspase-3表达均升高,两组比较差异无统计学意义(P＞0.05).此外,湿热内蕴型、脾胃虚弱型的Caspase-3、Bax的表达均呈正相关(r=0.23、r=0.21).结论 Bax、Caspase-3 在UC中呈高表达,两者在细胞凋亡中具有调节作用,并与辨证分型有关.
목적 탐토궤양성결장염(ulcerative colitis,UC)중반광천동안산매-3(Caspase-3)、B세포림파류/백혈병기인2상관X단백(Bax)적표체수평여변증분형적상관성.방법 장UC환자변증분위습열내온형조(25례)、비위허약형조(25례),용면역조직화학기술(SP법),검측량조환자결장점막선상피세포Caspase-3、Bax적표체수평정황；병여정상대조조(25례)진행비교.결과 Bax재습열내온형[(4.56±1.58)분、비위허약형(3.28±1.14)분]적표체균승고,여정상대조조비교,차이균유통계학의의(P균＜0.0.5)；량형적Caspase-3표체균승고,량조비교차이무통계학의의(P＞0.05).차외,습열내온형、비위허약형적Caspase-3、Bax적표체균정정상관(r=0.23、r=0.21).결론 Bax、Caspase-3 재UC중정고표체,량자재세포조망중구유조절작용,병여변증분형유관.
Objective To study the correlation between TCM differentiation of syndromes typing for ulcerative colitis (UC) and expressions of Bax and Caspase-3.Methods 50 patients with UC were divided into 2 groups according to TCM syndrome differentiation including dampness-heat internal accumulation syndrome group (25cases),deficiency of spleen and stomach syndrome group (25cases).Immunohistochemistry was used to study expression of Bax and Caspase-3 in colonic mucosas and compare with the normal group (25cases).Results The expressions of Bax were highest in dampness-heat internal accumulation syndrome group (4.56±1.58).The expressions of Bax of deficiency of spleen and stomach syndrome group were lower than dampness-heat internal accumulation syndrome group(3.28± 1.14)scores.There were significant differences between the three groups (P＜0.05).However,There were no significant difference between dampness-heat internal accumulation syndrome group and deficiency of spleen and stomach syndrome group in terms of the Caspase-3expressions (P＞0.05).In addition,Caspase-3 and Bax were positively related in each group(r=0.23and 0.21).Conclusion The high expressions of Bax,Caspase-3 in ulcerative colitis were closely related to TCM typing according to syndrome differentiation.Caspase-3 and Bax were adjustment in apoptosis.The detection of expression of Bax,Caspase-3 is helpful to the TCM syndrome differentiation and to the selection of treatment plan.At the same time,it can provide more important experiment basses for the pathogenesis of ulcerative colitis.