CAJ | 학술논문

目的研究灵芝多糖与顺铂联合用药对荷瘤小鼠的肿瘤生长情况及其免疫功能的影响.方法 Balb/c小鼠腋下接种小鼠肺癌细胞株Lewis 5×106个细胞,待肿瘤至100 mm3分组给药.分为对照组、灵芝多糖组(1次/2 d,40 mg/kg,灌胃)、顺铂组(1次/2 d,2 mg/kg,腹腔注射)和联合用药组(等剂量的顺铂和灵芝多糖).每3天测量一次肿瘤的体积,30d后解剖,检测荷瘤小鼠的肿瘤大小、脾脏的变化,脾脏和外周血中免疫细胞的变化以及相关免疫细胞的功能.结果灵芝多糖可有效增加小剂量顺铂的抗肿瘤效果,提高荷瘤小鼠的存活率,使小鼠外周血中Th细胞向Th1转化,增加细胞免疫功能,并增加骨髓来源DC细胞表面CD11c分子的表达(与对照组比较灵芝多糖组从1.06％升至4.59％,与顺铂比较,联合用药组从2.8％升至7.21％),提高抗原提呈能力.结论灵芝多糖可以提高荷瘤小鼠的免疫功能,与小剂量顺铂联合用药后,具有协同抗肿瘤生长作用,且可提高小鼠存活率.
목적 연구령지다당여순박연합용약대하류소서적종류생장정황급기면역공능적영향.방법 Balb/c소서액하접충소서폐암세포주Lewis 5×106개세포,대종류지100 mm3분조급약.분위대조조、령지다당조(1차/2 d,40 mg/kg,관위)、순박조(1차/2 d,2 mg/kg,복강주사)화연합용약조(등제량적순박화령지다당).매3천측량일차종류적체적,30d후해부,검측하류소서적종류대소、비장적변화,비장화외주혈중면역세포적변화이급상관면역세포적공능.결과 령지다당가유효증가소제량순박적항종류효과,제고하류소서적존활솔,사소서외주혈중Th세포향Th1전화,증가세포면역공능,병증가골수래원DC세포표면CD11c분자적표체(여대조조비교령지다당조종1.06％승지4.59％,여순박비교,연합용약조종2.8％승지7.21％),제고항원제정능력.결론 령지다당가이제고하류소서적면역공능,여소제량순박연합용약후,구유협동항종류생장작용,차가제고소서존활솔.
Objective To study the effects on inhibit tumor growth and enhance immune function of Ganoderma lucidum polysaccharide(GLP)combined with low-dose cisplatin in tumor-bearing mice.Methods 2 × 106 Lewis cells in 0.1 mL PBS were injected subcutaneously into the flanks of healthy female BALB/c nude mice (5～6 weeks old).When the tumor volume reached 100 mm3,mice were randomly assigned to 4 groups,control group (Oral gavage of 0.5 ml PBS and intraperitoneal injection of 0.5 ml),GLP group (gavage once every two days,40 mg/kg GLP in 0.5 ml),cisplatin group (intraperitoneal injection once every two days,2 mg/kg cisplatin in 0.5 ml) and the combination group (such as the above dose of cisplatin and GLP).After the treatment regimen was complete,the mice were sacrificed,and the tumors were removed,photographed,weighed,and prepared for histological analysis.The immune cells in spleen and peripheral blood were analyzed by flow cytometry.Results GLP can effectively increase anti-tumor effect of the small dose cisplatin,improve the survival of tumor-bearing mice,promote Th cells convert to Th1 in peripheral blood,increased the expression of CD 11c + on DC cell surface (GLP group 4.59％ VS the control group 1.06％,the combined group 7.21％ VS the cisplatin group 2.8％).Conclusion GLP can improve the immune function of tumor-bearing mice,had synergistic anti-tumor growth with low dose cisplatin,moreover,reduce renal toxic effects of cisplatin.