国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2012年
12期
1088-1090
,共3页
何惠%刘基铎%周迎春%肖明锋%蓝红云%刘光平
何惠%劉基鐸%週迎春%肖明鋒%藍紅雲%劉光平
하혜%류기탁%주영춘%초명봉%람홍운%류광평
慢性髓系细胞白血病%K562细胞%细胞凋亡%瘀毒清
慢性髓繫細胞白血病%K562細胞%細胞凋亡%瘀毒清
만성수계세포백혈병%K562세포%세포조망%어독청
Chronic myeloid leukemia%K562 cells%Aptoptosis%Yuduqing
目的 探讨瘀毒清对诱导慢性粒细胞白血病K562细胞株凋亡的作用.方法 采用血清药理学方法,取慢性粒细胞白血病K562细胞株悬液分组,分别加入不含药血清(空白对照组)、含伊马替尼血清、含高剂量瘀毒清血清、中剂量瘀毒清血清、低剂量瘀毒清血清、含伊马替尼加高、中、低剂量瘀毒清血清,不加血清作为正常对照组.分别于干预后第12h、24 h、48 h和72 h通过Annexin V/PI双染法、流式细胞仪检测不同时间、不同药物组的细胞凋亡率.结果 正常对照组的原代细胞有较低凋亡率,而空白对照组K562细胞的凋亡率更低,二者比较差异有统计学意义(P<0.05).12h、24 h内瘀毒清含药血清低、中、高组凋亡率与正常对照组、空白对照组比较存在统计学差异(P<0.05).药物组间相互比较亦有显著差异,并呈一定的时间剂量依赖性.瘀毒清高剂量组72 h未见凋亡率(31.48±6.58)进一步升高,与48 h比较无统计学差异,与瘀毒清中剂量组72 h的诱导凋亡率(27.54±5.89)比较也无统计学差异(P>0.05).伊马替尼组12h开始即显示较高的凋亡率(23.80±6.94),与空白对照组细胞的凋亡率相比有统计学差异(P<0.05).伊马替尼加瘀毒清各剂量组72 h的凋亡率与伊马替尼组、瘀毒清低、中、高组的凋亡率比较均有明显差异(P<0.05).结论 含瘀毒清血清对体外K562细胞有诱导凋亡作用,其作用呈一定时间、剂量依赖关系:瘀毒清与伊马替尼联合使用有增效作用.
目的 探討瘀毒清對誘導慢性粒細胞白血病K562細胞株凋亡的作用.方法 採用血清藥理學方法,取慢性粒細胞白血病K562細胞株懸液分組,分彆加入不含藥血清(空白對照組)、含伊馬替尼血清、含高劑量瘀毒清血清、中劑量瘀毒清血清、低劑量瘀毒清血清、含伊馬替尼加高、中、低劑量瘀毒清血清,不加血清作為正常對照組.分彆于榦預後第12h、24 h、48 h和72 h通過Annexin V/PI雙染法、流式細胞儀檢測不同時間、不同藥物組的細胞凋亡率.結果 正常對照組的原代細胞有較低凋亡率,而空白對照組K562細胞的凋亡率更低,二者比較差異有統計學意義(P<0.05).12h、24 h內瘀毒清含藥血清低、中、高組凋亡率與正常對照組、空白對照組比較存在統計學差異(P<0.05).藥物組間相互比較亦有顯著差異,併呈一定的時間劑量依賴性.瘀毒清高劑量組72 h未見凋亡率(31.48±6.58)進一步升高,與48 h比較無統計學差異,與瘀毒清中劑量組72 h的誘導凋亡率(27.54±5.89)比較也無統計學差異(P>0.05).伊馬替尼組12h開始即顯示較高的凋亡率(23.80±6.94),與空白對照組細胞的凋亡率相比有統計學差異(P<0.05).伊馬替尼加瘀毒清各劑量組72 h的凋亡率與伊馬替尼組、瘀毒清低、中、高組的凋亡率比較均有明顯差異(P<0.05).結論 含瘀毒清血清對體外K562細胞有誘導凋亡作用,其作用呈一定時間、劑量依賴關繫:瘀毒清與伊馬替尼聯閤使用有增效作用.
목적 탐토어독청대유도만성립세포백혈병K562세포주조망적작용.방법 채용혈청약이학방법,취만성립세포백혈병K562세포주현액분조,분별가입불함약혈청(공백대조조)、함이마체니혈청、함고제량어독청혈청、중제량어독청혈청、저제량어독청혈청、함이마체니가고、중、저제량어독청혈청,불가혈청작위정상대조조.분별우간예후제12h、24 h、48 h화72 h통과Annexin V/PI쌍염법、류식세포의검측불동시간、불동약물조적세포조망솔.결과 정상대조조적원대세포유교저조망솔,이공백대조조K562세포적조망솔경저,이자비교차이유통계학의의(P<0.05).12h、24 h내어독청함약혈청저、중、고조조망솔여정상대조조、공백대조조비교존재통계학차이(P<0.05).약물조간상호비교역유현저차이,병정일정적시간제량의뢰성.어독청고제량조72 h미견조망솔(31.48±6.58)진일보승고,여48 h비교무통계학차이,여어독청중제량조72 h적유도조망솔(27.54±5.89)비교야무통계학차이(P>0.05).이마체니조12h개시즉현시교고적조망솔(23.80±6.94),여공백대조조세포적조망솔상비유통계학차이(P<0.05).이마체니가어독청각제량조72 h적조망솔여이마체니조、어독청저、중、고조적조망솔비교균유명현차이(P<0.05).결론 함어독청혈청대체외K562세포유유도조망작용,기작용정일정시간、제량의뢰관계:어독청여이마체니연합사용유증효작용.
Objective To explore the effect of Yuduqing on the apoptosis of CML K562 cells cultured in vitro and its molecular mechanism.Methods In serologic pharmacological test,the K562 cells were divided into 8 different groups.Serum with imatinib plus Yuduqing (high-dose,middle-dose,low-dose) were added into the cells respectively in the 8 groups of K562 cells.Morphological assessment of apoptosis was performed with optical microscope,the rates of apoptosis and the cell cycles analysis was performed with flow cytometry at 12 h,24 h,48 h and 72 h time points respectively after the intervention.Results The primary cell of normal control group had a low rate of apoptosis,while the blank control group K562 cell apoptosis rate was lower,the difference is significant (P<0.05).The differences between the rates of apoptosis in high-dose,middle-dose and low-dose Yuduqing groups and those in normal control group and blank control group were significant in 12 hours or 24 hours (P<0.05).Drug groups showed significant differences of pair-comparison in groups and a certain time dose dependence.But the rate of apoptosis(31.48± 6.58) in k562 cells in high-dose Yuduqing group did not increase further at 72 hours after the intervention and it was not statistically different from that of 48 hours,nor statistically different from that of middle-dose group at 72 hours(27.54±5.89) after the intervention (P>0.05).The rate ofapoptosis in k562 cells in imatinib group (23.80±6.94) was relatively high at 12 hours after the intervention and it was significantly different from that in blank control group (P<0.05).The rates of apoptosis in imatinib and Yuduqing (high-dose,middle-dose,and low-dose) groups were significantly higher than those in imatinib group or Yuduqing high-dose,middle-dose,and low-dose)groups (P<0.05).Conclusion Serum with Yuduqing could induce apoptosis of K562 cells cultured in vitro and its action was dose-time dependent; Serum with Yuduqing (high-dose and middle-dose) was similar to serum with imatinib in inducing apoptosis of K562 cells cultured in vitro; Yuduqing could enhance the efficacy of imatinib.