国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2013年
3期
228-230
,共3页
孙跃宁%杜连平%孙楠%王海英
孫躍寧%杜連平%孫楠%王海英
손약저%두련평%손남%왕해영
玛奴西汤%质量标准%薄层色谱法%高压液相色谱法%异土木香内酯%土木香内酯
瑪奴西湯%質量標準%薄層色譜法%高壓液相色譜法%異土木香內酯%土木香內酯
마노서탕%질량표준%박층색보법%고압액상색보법%이토목향내지%토목향내지
MNXT granule%Quality standard%TLC%HPLC%Isoalantolactone%Alantolactone
目的 建立玛奴西汤(MNXT granule)的质量控制标准.方法 采用薄层色谱法(TLC)对制剂中藏木香、宽筋藤进行定性鉴别；采用高效液相色谱法对制剂中藏木香的成分异土木香内酯、土木香内酯进行定量测定.结果 玛奴西汤中藏木香、宽筋藤经TLC鉴别,方法 专属性强,土木香内酯、异土木香内酯经HPLC含量测定,方法 专属性强.可鉴别藏木香、宽筋藤,阴性对照无干扰,方法 专属性强.异土木香内酯在0.281～0.842 μg范围内呈良好的线性关系,r=0.9998,平均回收率(n=5)为98.5％,RSD为1.14％；土木香内酯在0.232～0.696μg范围内呈良好的线性关系,r=0.9999,平均回收率(n=5)为97.4％,RSD为1.10％.结论 该方法 简便、准确、重复性好,可有效控制玛奴西汤的质量.
目的 建立瑪奴西湯(MNXT granule)的質量控製標準.方法 採用薄層色譜法(TLC)對製劑中藏木香、寬觔籐進行定性鑒彆；採用高效液相色譜法對製劑中藏木香的成分異土木香內酯、土木香內酯進行定量測定.結果 瑪奴西湯中藏木香、寬觔籐經TLC鑒彆,方法 專屬性彊,土木香內酯、異土木香內酯經HPLC含量測定,方法 專屬性彊.可鑒彆藏木香、寬觔籐,陰性對照無榦擾,方法 專屬性彊.異土木香內酯在0.281～0.842 μg範圍內呈良好的線性關繫,r=0.9998,平均迴收率(n=5)為98.5％,RSD為1.14％；土木香內酯在0.232～0.696μg範圍內呈良好的線性關繫,r=0.9999,平均迴收率(n=5)為97.4％,RSD為1.10％.結論 該方法 簡便、準確、重複性好,可有效控製瑪奴西湯的質量.
목적 건립마노서탕(MNXT granule)적질량공제표준.방법 채용박층색보법(TLC)대제제중장목향、관근등진행정성감별；채용고효액상색보법대제제중장목향적성분이토목향내지、토목향내지진행정량측정.결과 마노서탕중장목향、관근등경TLC감별,방법 전속성강,토목향내지、이토목향내지경HPLC함량측정,방법 전속성강.가감별장목향、관근등,음성대조무간우,방법 전속성강.이토목향내지재0.281～0.842 μg범위내정량호적선성관계,r=0.9998,평균회수솔(n=5)위98.5％,RSD위1.14％；토목향내지재0.232～0.696μg범위내정량호적선성관계,r=0.9999,평균회수솔(n=5)위97.4％,RSD위1.10％.결론 해방법 간편、준학、중복성호,가유효공제마노서탕적질량.
Objective To establish the quality standard for Tibetan medicine MNXT granule.Methods Inula racemosa,Tinospora sinensis were identified by TLC; Isoalantolactone and alantolactone were determined by HPLC.Results Inula racemosa,Tinospora sinensis could be identified by TLC.The concentration of isoalantolactone was linear in the range of 0.281～0.842 μg,r=0.9998,with the average recovery rate being 98.5％,RSD being1.14％.The concentration of alantolactone was linear in the range of 0.232～0.696 μg,r=0.9999,with the average recovery rate being 97.4％,RSD being 1.10％.Conclusion The method was simple,accurate,repeatable and able to control the quality of preparation effectively.
