CAJ | 학술논문

目的观察中药复方糖痹康对糖尿病周围神经病变大鼠的抗氧化作用.方法采用链脲佐菌素制作糖尿病周围神经病变(DPN)大鼠模型,将成模大鼠随机分为模型对照组、甲钴胺组,以及糖痹康低、中、高剂量组.观察各组连续给药12周后,大鼠血浆、肝脏、肌肉和神经组织中的MDA和SOD水平,并观察坐骨神经的超微结构.结果糖痹康低、中、高剂量组DPN大鼠肝组织中MDA含量[(8.47±1.82) nmol/ml、(7.57±1.45) nmol/ml、(8.71±1.51) nmol/ml]较模型组[(10.42±2.05) nmol/ml]明显降低(P＜0.01或＜0.05),SOD含量低、中、高剂量组[(21.12±1.52) U/ml、(22.29±2.21)U/ml、(19.29±1.10) U/ml]较模型组[(18.24±1.98) U/ml]明显升高(P＜0.01或0.05)；糖痹康各剂量组还可不同程度的降低DPN大鼠血浆、肌肉以及神经组织中的MDA含量,与模型组比较差异有统计学意义(P＜0.05),且能升高血浆、肌肉和神经组织中的SOD含量,与模型组比较差异有统计学意义(P＜0.01).经糖痹康治疗后糖尿病周围神经病变大鼠的透射电镜显示:坐骨神经有髓神经纤维变宽的髓鞘和松散的结构明显改善,斑块状脱髓鞘和节段性脱髓鞘现象明显减少,空泡状缺损少见,神经微丝、微管清晰可见,髓鞘结构趋完整态分布.结论糖痹康对糖尿病周围神经病变具有抗氧化作用,可减轻氧化应激造成的周围神经结构和功能损害,对DPN有较好地神经保护作用.
목적 관찰중약복방당비강대당뇨병주위신경병변대서적항양화작용.방법 채용련뇨좌균소제작당뇨병주위신경병변(DPN)대서모형,장성모대서수궤분위모형대조조、갑고알조,이급당비강저、중、고제량조.관찰각조련속급약12주후,대서혈장、간장、기육화신경조직중적MDA화SOD수평,병관찰좌골신경적초미결구.결과 당비강저、중、고제량조DPN대서간조직중MDA함량[(8.47±1.82) nmol/ml、(7.57±1.45) nmol/ml、(8.71±1.51) nmol/ml]교모형조[(10.42±2.05) nmol/ml]명현강저(P＜0.01혹＜0.05),SOD함량저、중、고제량조[(21.12±1.52) U/ml、(22.29±2.21)U/ml、(19.29±1.10) U/ml]교모형조[(18.24±1.98) U/ml]명현승고(P＜0.01혹0.05)；당비강각제량조환가불동정도적강저DPN대서혈장、기육이급신경조직중적MDA함량,여모형조비교차이유통계학의의(P＜0.05),차능승고혈장、기육화신경조직중적SOD함량,여모형조비교차이유통계학의의(P＜0.01).경당비강치료후당뇨병주위신경병변대서적투사전경현시:좌골신경유수신경섬유변관적수초화송산적결구명현개선,반괴상탈수초화절단성탈수초현상명현감소,공포상결손소견,신경미사、미관청석가견,수초결구추완정태분포.결론 당비강대당뇨병주위신경병변구유항양화작용,가감경양화응격조성적주위신경결구화공능손해,대DPN유교호지신경보호작용.
Objective To evaluate the antioxidant effects of compound Chinese medicine Tangbikang's on diabetic peripheral neuropathy rat.Methods The rats of diabetic peripheral neuropathy (DPN) were induced by streptozotocin intraperitoneally.The DPN rats were divided into 5 groups:model group,diabetic peripheral neuropathy rats treated with mecobalamin,DPN rats treated with the low dose of Tangbikang,DPN rats treated with the medium dose of Tangbi-Kang,and DPN rats treated with the high dose of Tangbikang.MDA and SOD levels in plasma,liver,muscels and nervous tissues were detected after continuously for 12 weeks,and the ultrastructure of sciatic never in rats were observed.Results The different dose of Tangbikang could reduce the contents of MDA in liver [(8.47± 1.82,7.57± 1.45,8.71 ± 1.51)nmol/ml] compared with model group [(10.42±2.05) nmol/ml] with significant difference (P＜0.01,P＜0.05),increase the contents of SOD in live r[(21.12± 1.52,22.29±2.21,19.29± 1.10) U/ml],compared with model group[(18.24± 1.98) U/ml] with significant difference (P＜0.01,P＜0.05),reduce the contents of MDA in plasma,muscels and nervous tissues,compared with model group,with significant difference (P＜0.05),and increase the contents of SOD in plasma,museels and nervous tissues on DPN rats,compared with model group,with significant difference (P＜0.05).After treated with Tangbikang,the results of transmission electron microscopy showed that the widely myelin sheath and loose structure of myelinated nerve fibers of sciatic nerve were improved significantly,plaque demyelinating and segmental demyelination phenomenon were reduced significantly,the vacuolus defects was rare,neurofilaments,neurotubule was visible clearly,the structure of myelin sheath tended to be complete and regular.Conclusion Tangbikang has antioxidant effect.It can reduce the damage of peripheral nerve structure and function by oxidative stress,and protect nerve of DPN rats.