国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2013年
7期
604-606
,共3页
溃疡%大鼠%复方丹参注射液%白细胞介素-1β
潰瘍%大鼠%複方丹參註射液%白細胞介素-1β
궤양%대서%복방단삼주사액%백세포개소-1β
Ulcer%Rat%Compound Danshen injection%Interleukin-1beta
目的 探讨复方丹参注射液对大鼠应激性胃黏膜损伤的保护作用及其机制.方法 采用浸水束缚应激法建立大鼠应激性胃黏膜损伤的动物模型.50只SD大鼠采用随机数字表法分为5组,即正常对照组、应激模型组、低剂量丹参治疗组、中剂量丹参治疗组、高剂量丹参治疗组.检测5组大鼠胃黏膜溃疡指数(UI)、胃黏膜丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、血浆白细胞介素(IL-1β含量).结果 应激模型组大鼠胃黏膜UI值为(28.4±4.5) mm、MDA含量为(0.60±0.08) nmol/mg,均高于正常对照组[分别为(0.7±0.4) mm、(0.25±0.03) nmol/mg],(P均＜0.01).胃黏膜SOD活性为(122.5±14.2) Nu/mg,低于正常对照组(246.5±45.4) Nu/mg (P＜0.01).应激模型组大鼠血浆IL-1β含量为(31.6±8.4) pg/ml,显著高于正常对照组(8.5±3.1) pg/ml (P＜0.01).应激前预先应用复方丹参注射液则呈剂量依赖性减轻了大鼠应激性胃黏膜损伤,降低UI值[(22.8±3.7) mm、(12.2±3.5) mm、(6.2±1.6) mm]、MDA含量[(0.52±0.07) nmol/mg、(0.32±0.06) nmol/mg、(0.28±0.03) nmol/mg],提高胃黏膜SOD活性[(135.2±13.6) Nu/mg、(220.7±33.5) Nu/mg、(251.2±23.7) Nu/mg],同时降低血浆IL-1β含量[(27.2±7.5) pg/ml、(13.5±5.3)pg/ml、(9.3±4.4) pg/ml] (P＜0.01).结论 复方丹参注射液对大鼠浸水束缚冷应激性胃黏膜损伤有预防作用.
目的 探討複方丹參註射液對大鼠應激性胃黏膜損傷的保護作用及其機製.方法 採用浸水束縳應激法建立大鼠應激性胃黏膜損傷的動物模型.50隻SD大鼠採用隨機數字錶法分為5組,即正常對照組、應激模型組、低劑量丹參治療組、中劑量丹參治療組、高劑量丹參治療組.檢測5組大鼠胃黏膜潰瘍指數(UI)、胃黏膜丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、血漿白細胞介素(IL-1β含量).結果 應激模型組大鼠胃黏膜UI值為(28.4±4.5) mm、MDA含量為(0.60±0.08) nmol/mg,均高于正常對照組[分彆為(0.7±0.4) mm、(0.25±0.03) nmol/mg],(P均＜0.01).胃黏膜SOD活性為(122.5±14.2) Nu/mg,低于正常對照組(246.5±45.4) Nu/mg (P＜0.01).應激模型組大鼠血漿IL-1β含量為(31.6±8.4) pg/ml,顯著高于正常對照組(8.5±3.1) pg/ml (P＜0.01).應激前預先應用複方丹參註射液則呈劑量依賴性減輕瞭大鼠應激性胃黏膜損傷,降低UI值[(22.8±3.7) mm、(12.2±3.5) mm、(6.2±1.6) mm]、MDA含量[(0.52±0.07) nmol/mg、(0.32±0.06) nmol/mg、(0.28±0.03) nmol/mg],提高胃黏膜SOD活性[(135.2±13.6) Nu/mg、(220.7±33.5) Nu/mg、(251.2±23.7) Nu/mg],同時降低血漿IL-1β含量[(27.2±7.5) pg/ml、(13.5±5.3)pg/ml、(9.3±4.4) pg/ml] (P＜0.01).結論 複方丹參註射液對大鼠浸水束縳冷應激性胃黏膜損傷有預防作用.
목적 탐토복방단삼주사액대대서응격성위점막손상적보호작용급기궤제.방법 채용침수속박응격법건립대서응격성위점막손상적동물모형.50지SD대서채용수궤수자표법분위5조,즉정상대조조、응격모형조、저제량단삼치료조、중제량단삼치료조、고제량단삼치료조.검측5조대서위점막궤양지수(UI)、위점막병이철(MDA)함량、초양화물기화매(SOD)활성、혈장백세포개소(IL-1β함량).결과 응격모형조대서위점막UI치위(28.4±4.5) mm、MDA함량위(0.60±0.08) nmol/mg,균고우정상대조조[분별위(0.7±0.4) mm、(0.25±0.03) nmol/mg],(P균＜0.01).위점막SOD활성위(122.5±14.2) Nu/mg,저우정상대조조(246.5±45.4) Nu/mg (P＜0.01).응격모형조대서혈장IL-1β함량위(31.6±8.4) pg/ml,현저고우정상대조조(8.5±3.1) pg/ml (P＜0.01).응격전예선응용복방단삼주사액칙정제량의뢰성감경료대서응격성위점막손상,강저UI치[(22.8±3.7) mm、(12.2±3.5) mm、(6.2±1.6) mm]、MDA함량[(0.52±0.07) nmol/mg、(0.32±0.06) nmol/mg、(0.28±0.03) nmol/mg],제고위점막SOD활성[(135.2±13.6) Nu/mg、(220.7±33.5) Nu/mg、(251.2±23.7) Nu/mg],동시강저혈장IL-1β함량[(27.2±7.5) pg/ml、(13.5±5.3)pg/ml、(9.3±4.4) pg/ml] (P＜0.01).결론 복방단삼주사액대대서침수속박랭응격성위점막손상유예방작용.
Objective To investigate the effects of compound Danshen injection on stress-induced gastric mucosal damage and its mechanism.Methods Sprague Dawley rats were used to establish immersion restraint cold stress model.50 SD rats were randomly divided into five groups(n=10 per group):① the normal control group; ② the stress model group; ③ the group treated with low dose of compound Danshen injection(50 mg/kg) ; ④ the group treated with medial dose of compound Danshen injection(100 mg/kg) ; ⑤the group treated with high dose of compound Danshen injection (200 mg/kg).The gastric mucosal ulcer index (UI),the level of malondialdehyde (MDA) in gastric mucosa,the activity of superoxide dismutase (SOD),and plasma intedeukin-1beta (IL-1β)content were detected.Results Compared with the normal control group [(0.7± 0.4) mm,(0.25 ± 0.03)nmol/mg,and (246.5 ± 45.4)Nu/mg],the stress model group exhibited a markedly increase in gastric mucosal UI[(28.4±4.5)mm](P＜0.01) and MDA level [(0.60±0.08)nmol/mg](P＜0.01) and a decrease in the activity of gastric mucosal SOD [(122.5 ±14.2) Nu/mg] (P＜0.01).In addition,an elevated level of plasma IL-1β[(31.6±8.4) pg/ml vs (8.5±3.1) pg/ml] (P＜0.01) was observed in the stress model group.Pretreatment with compound Danshen injection dose-dependently attenuated the gastric mucosal damage,characterized by a markedly decrease in gastric mucosal UI [(22.8 ± 3.7)mm、(12.2 ±3.5)mm,(6.2 ± 1.6)mm] and MDA level [(0.52 ± 0.07)nmol/mg,(0.32 ± 0.06)nmol/mg,(0.28 ±0.03)nmol/mg] and an increase in the activity of gastric mucosal SOD[(135.2± 13.6)Nu/mg,(220.7±33.5)Nu/mg,(251.2±23.7)Nu/mg] (P＜0.01).Meanwhile,compound Danshen injection reduced the content of plasma IL-1β[(27.2±7.5)pg/ml,(13.5±5.3)pg/ml,(9.3±4.4)pg/ml] (P＜0.01).Conclusion Compound Danshen injection exhibits preventive protection on the stress-induced gastric mucosal damage by water immersion restraint cold stress in SD rats,the mechanism of which might be linked to its inhibition of gastric mucosal oxidation stress and the reduced release of inflammatory mediator IL-1β.
