国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2013年
7期
610-612
,共3页
二十五味珊瑚胶囊%颈椎病%退变颈椎间盘%磷脂酶A2
二十五味珊瑚膠囊%頸椎病%退變頸椎間盤%燐脂酶A2
이십오미산호효낭%경추병%퇴변경추간반%린지매A2
Jinzhuyalong Ershiwuwei coral capsule%Cervical spondylosis%Degenerated intervertebral discs%Phospho-lipaseA2
目的 观察二十五味珊瑚胶囊对模型动物退变颈椎间盘组织磷脂酶A2 (PLA2)活性的影响,探讨二十五味珊瑚胶囊治疗颈椎病的作用机理.方法 SD大鼠(SPF) 60只,随机抽签分为对照组(15只)、模型对照组(15只)、模型服药1组(15只),模型服药2组(15只)；模型服药1、2组分别给予二十五味珊瑚胶囊粉剂0.33×5g/kg、0.66×5g/kg,加蒸馏水1ml稀释灌胃给药,1次/d,持续90 d.选用动力失衡性颈椎病动物模型,90 d后取C4～5颈椎间盘,采用催化活性与微孔比色相结合的方法测定PLA2活性.结果 模型组颈椎间盘PLA2活性(12.37±4.21)IU/mg·min-1,明显高于对照组(2.56±1.19) IU/mg·min-1 (P＜ 0.05).模型服药1组、模型服药2组颈椎间盘PLA2活性分别为(8.56±2.13) IU/mg·min-1、(5.58±2.39) IU/mg·min-1,均低于模型对照组(12.37±4.21) IU/mg· min-1(P均＜0.01).结论 二十五味珊瑚胶囊可下调退变颈椎间盘中PLA2的活性,可减少多种炎症介质的合成.
目的 觀察二十五味珊瑚膠囊對模型動物退變頸椎間盤組織燐脂酶A2 (PLA2)活性的影響,探討二十五味珊瑚膠囊治療頸椎病的作用機理.方法 SD大鼠(SPF) 60隻,隨機抽籤分為對照組(15隻)、模型對照組(15隻)、模型服藥1組(15隻),模型服藥2組(15隻)；模型服藥1、2組分彆給予二十五味珊瑚膠囊粉劑0.33×5g/kg、0.66×5g/kg,加蒸餾水1ml稀釋灌胃給藥,1次/d,持續90 d.選用動力失衡性頸椎病動物模型,90 d後取C4～5頸椎間盤,採用催化活性與微孔比色相結閤的方法測定PLA2活性.結果 模型組頸椎間盤PLA2活性(12.37±4.21)IU/mg·min-1,明顯高于對照組(2.56±1.19) IU/mg·min-1 (P＜ 0.05).模型服藥1組、模型服藥2組頸椎間盤PLA2活性分彆為(8.56±2.13) IU/mg·min-1、(5.58±2.39) IU/mg·min-1,均低于模型對照組(12.37±4.21) IU/mg· min-1(P均＜0.01).結論 二十五味珊瑚膠囊可下調退變頸椎間盤中PLA2的活性,可減少多種炎癥介質的閤成.
목적 관찰이십오미산호효낭대모형동물퇴변경추간반조직린지매A2 (PLA2)활성적영향,탐토이십오미산호효낭치료경추병적작용궤리.방법 SD대서(SPF) 60지,수궤추첨분위대조조(15지)、모형대조조(15지)、모형복약1조(15지),모형복약2조(15지)；모형복약1、2조분별급여이십오미산호효낭분제0.33×5g/kg、0.66×5g/kg,가증류수1ml희석관위급약,1차/d,지속90 d.선용동력실형성경추병동물모형,90 d후취C4～5경추간반,채용최화활성여미공비색상결합적방법측정PLA2활성.결과 모형조경추간반PLA2활성(12.37±4.21)IU/mg·min-1,명현고우대조조(2.56±1.19) IU/mg·min-1 (P＜ 0.05).모형복약1조、모형복약2조경추간반PLA2활성분별위(8.56±2.13) IU/mg·min-1、(5.58±2.39) IU/mg·min-1,균저우모형대조조(12.37±4.21) IU/mg· min-1(P균＜0.01).결론 이십오미산호효낭가하조퇴변경추간반중PLA2적활성,가감소다충염증개질적합성.
Objective To study the anti-inflammation mechanism of Jinzhuyalong Ershiwuwei coral capsule on cervical spondylosis by investigating Jinzhuyalong Ershiwuwei coral capsule on PLA2 in degenerated cervical intervertebral discs.Methods 60SD rats (SPF) were divided into a control group (15),a medicine model control group (15),a medicine modle 1 group (15),and a medicine model 2 group (15) by random lottery; Medicine model 1,2 groups were given Jinzhuyalong Ershiwuwei coral capsule powder 0.33 × 5 g/kg 0.66×5 g/kg,and added 1 ml distilled water dilution lavage,l/d,90 d,The unbalance of power cervical spondylosis animal models were chosen and C4～5 neck intervertebral discs were taken after 90 days.Millipore colorimetry methods were used to measure PLA2 activity in C4～5 cervical intervertebral discs.Results The PLA2 activity of the cervical intervertebral disc (12.37 ± 4.21) IU/mg · min-1 in the model group was significantly higher than the control group(2.56± 1.19)IU/mg· min-1 (P＜0.05).The cervical intervertebral disc PLA2 activity of medicine model group 1 (8.56± 2.13)IU/mg · min-1 and medicine model group 2 (5.58 ±2.39)IU/mg· min-1 was lower than the model control group(12.37±4.21)IU/mg· min-1,with significant differences (both P＜0.01).Conclusion Jinzhuyalong Ershiwuwei coral capsule could down regulate PLA2 activity in degenerated cervical intervertebral discs,and also decrease the production of some inflammaroty mediators.
