国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2013年
11期
993-996
,共4页
儿茶素%重症急性胰腺炎%肠黏膜屏障功能%氧自由基
兒茶素%重癥急性胰腺炎%腸黏膜屏障功能%氧自由基
인다소%중증급성이선염%장점막병장공능%양자유기
EGCG%Severe acute pancreatitis%Intestinal mucosa barrier function%Free radicals
目的 本实验研究儿茶素对重症急性胰腺炎模型大鼠肠黏膜屏障功能损伤的保护作用及其机制,从而为重症急性胰腺炎的临床防治提供新的药物及思路.方法 80只清洁级SD大鼠按随机数字表分为正常对照组(8只)、假手术组(24只)、重症急性胰腺炎模型组(24只)和儿茶素(EGCG,24只)组.正常对照组不做任何处理；假手术组在开腹后仅翻动胰腺后关腹；急性胰腺炎模型组采用逆行十二指肠胰胆管注射5％牛黄胆酸钠溶液制备重症急性胰腺炎大鼠模型；儿茶素组在模型制备成功后即刻经尾静脉注射30mg/kg的儿茶素单体成分EGCG.各组大鼠分别在建模成功后6h、12h、24 h采集血液、肠道标本；检测血浆二胺氧化酶(DAO)、丙二醛(MDA)、一氧化氮(NO)含量；采用免疫组织化学法检测诱导型一氧化氮合酶(iNOS)的表达水平.结果 ①急性胰腺炎模型组术后6h、12 h、24 h血浆DAO浓度分别[(4.23±0.51)μ/L、(5.13±0.32)μ/L、(7.83±0.76) μ/L]、血淀粉酶浓度[(3158.64±218.25) μ/L、4277.24±231.35) μ/L、5842.76±248.18) μ/L]均明显高于正常对照组[DAO:(2.32±0.48)μ/L;血淀粉酶:(2074.11±101.56) μ/L]、假手术组[DAO:(2.24±0.43) u/L、(2.27.53) μ/L、(2.33±0.41) μ/L;血淀粉酶(1885.27±103.89)μ/L、(1778.57±98.94)u/L、(1935.24±105.87) μ/L].儿茶素组血浆DAO[(3.59±0.57) μ/L、(3.98±0.67) μ/L、(6.44±0.71)μ/L]、血淀粉酶浓度[(2576.70±123.43) μ/L、(2783.77±155.53)μ/L、(2902.32±176.25)μ/L]高于正常对照组、假手术组,但低于模型组.②与正常对照组、假手术组相比较,模型组肠道MDA、NO水平3个时间点依次升高[MDA:(2.28±0.35) μmol/g、(3.02±0.41) μmol/g、(3.78±0.48) μmol/g; NO:(16.80±0.60) μmol/g、(18.23±0.78) μmol/g、(20.14±0.82) μmol/g];儿茶素组MDA:(1.67±0.14) μmol/g、(1.75±0.16) μ.mol/g、(1.84±0.22) μmol/g; NO:(9.09±0.31) μmol/g、(9.32±0.44)μmol/g、(10.15±0.52) μmol/g较急性胰腺炎模型组肠道MDA、NO水平均明显降低.③重症急性胰腺炎组肠道iNOS表达水平(1.86)均高于正常对照组(0.10)、假手术组(0.25)；儿茶素治疗组肠道iNOS表达水平(0.66)低于模型组,但高于正常对照组、假手术组(P＜0.05).结论 儿茶素对重症急性胰腺炎大鼠肠黏膜屏障功能损害具有保护作用,机制与抗氧化、清除氧自由基及下调iNOS表达有关.
目的 本實驗研究兒茶素對重癥急性胰腺炎模型大鼠腸黏膜屏障功能損傷的保護作用及其機製,從而為重癥急性胰腺炎的臨床防治提供新的藥物及思路.方法 80隻清潔級SD大鼠按隨機數字錶分為正常對照組(8隻)、假手術組(24隻)、重癥急性胰腺炎模型組(24隻)和兒茶素(EGCG,24隻)組.正常對照組不做任何處理；假手術組在開腹後僅翻動胰腺後關腹；急性胰腺炎模型組採用逆行十二指腸胰膽管註射5％牛黃膽痠鈉溶液製備重癥急性胰腺炎大鼠模型；兒茶素組在模型製備成功後即刻經尾靜脈註射30mg/kg的兒茶素單體成分EGCG.各組大鼠分彆在建模成功後6h、12h、24 h採集血液、腸道標本；檢測血漿二胺氧化酶(DAO)、丙二醛(MDA)、一氧化氮(NO)含量；採用免疫組織化學法檢測誘導型一氧化氮閤酶(iNOS)的錶達水平.結果 ①急性胰腺炎模型組術後6h、12 h、24 h血漿DAO濃度分彆[(4.23±0.51)μ/L、(5.13±0.32)μ/L、(7.83±0.76) μ/L]、血澱粉酶濃度[(3158.64±218.25) μ/L、4277.24±231.35) μ/L、5842.76±248.18) μ/L]均明顯高于正常對照組[DAO:(2.32±0.48)μ/L;血澱粉酶:(2074.11±101.56) μ/L]、假手術組[DAO:(2.24±0.43) u/L、(2.27.53) μ/L、(2.33±0.41) μ/L;血澱粉酶(1885.27±103.89)μ/L、(1778.57±98.94)u/L、(1935.24±105.87) μ/L].兒茶素組血漿DAO[(3.59±0.57) μ/L、(3.98±0.67) μ/L、(6.44±0.71)μ/L]、血澱粉酶濃度[(2576.70±123.43) μ/L、(2783.77±155.53)μ/L、(2902.32±176.25)μ/L]高于正常對照組、假手術組,但低于模型組.②與正常對照組、假手術組相比較,模型組腸道MDA、NO水平3箇時間點依次升高[MDA:(2.28±0.35) μmol/g、(3.02±0.41) μmol/g、(3.78±0.48) μmol/g; NO:(16.80±0.60) μmol/g、(18.23±0.78) μmol/g、(20.14±0.82) μmol/g];兒茶素組MDA:(1.67±0.14) μmol/g、(1.75±0.16) μ.mol/g、(1.84±0.22) μmol/g; NO:(9.09±0.31) μmol/g、(9.32±0.44)μmol/g、(10.15±0.52) μmol/g較急性胰腺炎模型組腸道MDA、NO水平均明顯降低.③重癥急性胰腺炎組腸道iNOS錶達水平(1.86)均高于正常對照組(0.10)、假手術組(0.25)；兒茶素治療組腸道iNOS錶達水平(0.66)低于模型組,但高于正常對照組、假手術組(P＜0.05).結論 兒茶素對重癥急性胰腺炎大鼠腸黏膜屏障功能損害具有保護作用,機製與抗氧化、清除氧自由基及下調iNOS錶達有關.
목적 본실험연구인다소대중증급성이선염모형대서장점막병장공능손상적보호작용급기궤제,종이위중증급성이선염적림상방치제공신적약물급사로.방법 80지청길급SD대서안수궤수자표분위정상대조조(8지)、가수술조(24지)、중증급성이선염모형조(24지)화인다소(EGCG,24지)조.정상대조조불주임하처리；가수술조재개복후부번동이선후관복；급성이선염모형조채용역행십이지장이담관주사5％우황담산납용액제비중증급성이선염대서모형；인다소조재모형제비성공후즉각경미정맥주사30mg/kg적인다소단체성분EGCG.각조대서분별재건모성공후6h、12h、24 h채집혈액、장도표본；검측혈장이알양화매(DAO)、병이철(MDA)、일양화담(NO)함량；채용면역조직화학법검측유도형일양화담합매(iNOS)적표체수평.결과 ①급성이선염모형조술후6h、12 h、24 h혈장DAO농도분별[(4.23±0.51)μ/L、(5.13±0.32)μ/L、(7.83±0.76) μ/L]、혈정분매농도[(3158.64±218.25) μ/L、4277.24±231.35) μ/L、5842.76±248.18) μ/L]균명현고우정상대조조[DAO:(2.32±0.48)μ/L;혈정분매:(2074.11±101.56) μ/L]、가수술조[DAO:(2.24±0.43) u/L、(2.27.53) μ/L、(2.33±0.41) μ/L;혈정분매(1885.27±103.89)μ/L、(1778.57±98.94)u/L、(1935.24±105.87) μ/L].인다소조혈장DAO[(3.59±0.57) μ/L、(3.98±0.67) μ/L、(6.44±0.71)μ/L]、혈정분매농도[(2576.70±123.43) μ/L、(2783.77±155.53)μ/L、(2902.32±176.25)μ/L]고우정상대조조、가수술조,단저우모형조.②여정상대조조、가수술조상비교,모형조장도MDA、NO수평3개시간점의차승고[MDA:(2.28±0.35) μmol/g、(3.02±0.41) μmol/g、(3.78±0.48) μmol/g; NO:(16.80±0.60) μmol/g、(18.23±0.78) μmol/g、(20.14±0.82) μmol/g];인다소조MDA:(1.67±0.14) μmol/g、(1.75±0.16) μ.mol/g、(1.84±0.22) μmol/g; NO:(9.09±0.31) μmol/g、(9.32±0.44)μmol/g、(10.15±0.52) μmol/g교급성이선염모형조장도MDA、NO수평균명현강저.③중증급성이선염조장도iNOS표체수평(1.86)균고우정상대조조(0.10)、가수술조(0.25)；인다소치료조장도iNOS표체수평(0.66)저우모형조,단고우정상대조조、가수술조(P＜0.05).결론 인다소대중증급성이선염대서장점막병장공능손해구유보호작용,궤제여항양화、청제양자유기급하조iNOS표체유관.
Objective The study was designed to investigate the intestinal mucosa barrier function changes in rats with severe acute pancreatitis (SAP),and the intervention effect of epigallocatechin-3-gallate(EGCG) injection and its mechanisms of protection on intestinal mucosa barrier function injury of SAP with the purpose of providing evidence for preventing and curing intestinal mucosa barrier function injury of SAP in clinical practice.Methods A total of 80 healthy male Sprauge-Dawley (SD) rats were randomly divided into a normal group (Normal),a sham-operation group (Sham),a SAP model group (Model),and a EGCG treated group (EGCG),with 24 rats (except 8 rats in the normal group) in each group.The latter three groups each had three subgroups at three time points:6,12,24hours.Rats with SAP were prepared with retrograde cholangiopancreatic duct infusion in 5％ sodium taurocholate.The dynamic changes of the indexes of intestinal mucosa barrier function injury and Pancreas injury were observed at various time points in each group,including the levels of Plasma diamine oxidase (DAO) and serum amylase and malondialdehyde (MDA),nitric oxide(NO),inducible nitric oxide synthase(iNOS)in gut.Results 1.The levels of serum amylase [(3158.64 ± 218.25)μ/L,(4277.24 ± 231.35)μ/L,(5842.76 ± 248.18)μ/L] and plasma DAO concentration [(4.23 ± 0.51) μ/L,(5.13 ± 0.32)μ/L,(7.83 ± 0.76) μ/L] in the Model group were significantly higher at 6 h,12 h,24 h postoperation than the Normal group[serum amylase:(2074.11 ± 101.56)μ/L,DAO:(2.32 ± 0.48) μ/L] and Sham group [serum amylase:(1885.27 ± 103.89) μ/L,(1778.57 ± 98.94) μ/L,(1935.24 ± 105.87) μ/L].The levels of serum amylase [(2576.70 ± 123.43) μ/L,(2783.77 ± 155.53) μ/L,(2902.32 ±176.25) μ/L] and plasma DAO concentration (3.59 ± 0.57) μ/L,(3.98 ± 0.67) μ/L,(6.44 ± 0.71) μ/L in the EGCG group were lower than the Model group,but higher than the Normal group and the Sham group.Compared with the Normal group and the Sham group,the concentration of MDA and NO [MDA:(2.28±0.35) μmol/g,(3.02 ± 0.41) μmol/g,(3.78 ± 0.48) μmol/g ; NO:(16.80 ± 0.60) μmol/g,(18.23 ± 0.78) μmol/g,(20.14±0.82) μ,mol/g] significantly increased in the Model group at 6h,12 h,and 24 h postoperation.The concentration of MDA and NO (1.67±0.14) μmol/g,(1.75±0.16) μmol/g,(1.84±0.22) μmol/g; NO:(9.09±0.31) μmol/g,(9.32±0.44)μmol/g,(10.15±0.52)μmol/g were lower in the EGCG group in all times than the Model group.Compared with the Normal group (0.10) and the Sham group (0.25).The expression ofiNOS was significantly increased in the Model group(1.86).The expression of iNOS was lower in the EGCG group (0.66) in all times than the Model group,but higher than the Normal group and the sham group (P＜0.05).Conclusion Conclusion EGCG can protect the damages of intestinal mucosa barrier function in SAP rats,probably through reducing free radicals,and the iNOS expression.
