CAJ | 학술논문

目的观察绞股蓝皂苷对2型糖尿病合并非酒精性脂肪性肝病大鼠肝组织与血浆硫化氢(H2S)浓度的影响.方法 58只SPF级雄性SD大鼠按照体重相匹配的原则分为空白对照组(7只),2型糖尿病并非酒精性脂肪性肝病模型组(模型组,51只).建立2型糖尿病并非酒精性脂肪性肝病大鼠模型.2型糖尿病并非酒精性脂肪性肝病模型大鼠分别给予绞股蓝皂苷大剂量组(9只,1 g/kg·d-1),绞股蓝皂苷小剂量组(9只,0.5g/kg·d-1),2型糖尿病并非酒精性脂肪性肝病模型组(9只,模型组)以纯净水灌胃.检测肝组织、血浆硫化氢浓度变化,及血糖、甘油三酯、总胆固醇变化.结果 ①血浆硫化氢浓度:与模型组[(2.67±0.41) μmol/L]比较,绞股蓝皂苷大剂量组[(3.83±0.47) μmol/L]、小剂量组[(4.30±0.43) μmol/L]降低(P＜0.01).②肝组织硫化氢含量:与模型组[(237.8±33.05)pmol/min/mg/protein]比较,绞股蓝皂苷大剂量组[(275.81±36.07) pmol/min/mg/protein]、小剂量组[(333.52±37.94) pmol/min/mg/protein]升高.③血糖:与模型组(18.84±4.24) mmol/L比较,绞股蓝皂苷大剂量组(10.86±3.46) mmol/L、绞股蓝皂苷小剂量组(14.78±3.39) mmol/L降低(P＜0.01)；绞股蓝皂苷大剂量组低于小剂量组(P＜0.01).④甘油三酯水平:与模型组(3.97±0.64) mmol/L比较,空白对照组(0.96±0.09) mmol/L、绞股蓝皂苷大剂量组(1.83±0.56) mmol/L、绞股蓝皂苷小剂量组(2.82±0.66) mmol/L均降低(P均＜0.01).结论绞股蓝皂苷可升高2型糖尿病并非酒精性脂肪性肝病大鼠血浆、肝组织的硫化氢浓度,呈剂量依赖性,改善2型糖尿病并非酒精性脂肪性肝病大鼠血糖、血脂代谢.
목적 관찰교고람조감대2형당뇨병합병비주정성지방성간병대서간조직여혈장류화경(H2S)농도적영향.방법 58지SPF급웅성SD대서안조체중상필배적원칙분위공백대조조(7지),2형당뇨병병비주정성지방성간병모형조(모형조,51지).건립2형당뇨병병비주정성지방성간병대서모형.2형당뇨병병비주정성지방성간병모형대서분별급여교고람조감대제량조(9지,1 g/kg·d-1),교고람조감소제량조(9지,0.5g/kg·d-1),2형당뇨병병비주정성지방성간병모형조(9지,모형조)이순정수관위.검측간조직、혈장류화경농도변화,급혈당、감유삼지、총담고순변화.결과 ①혈장류화경농도:여모형조[(2.67±0.41) μmol/L]비교,교고람조감대제량조[(3.83±0.47) μmol/L]、소제량조[(4.30±0.43) μmol/L]강저(P＜0.01).②간조직류화경함량:여모형조[(237.8±33.05)pmol/min/mg/protein]비교,교고람조감대제량조[(275.81±36.07) pmol/min/mg/protein]、소제량조[(333.52±37.94) pmol/min/mg/protein]승고.③혈당:여모형조(18.84±4.24) mmol/L비교,교고람조감대제량조(10.86±3.46) mmol/L、교고람조감소제량조(14.78±3.39) mmol/L강저(P＜0.01)；교고람조감대제량조저우소제량조(P＜0.01).④감유삼지수평:여모형조(3.97±0.64) mmol/L비교,공백대조조(0.96±0.09) mmol/L、교고람조감대제량조(1.83±0.56) mmol/L、교고람조감소제량조(2.82±0.66) mmol/L균강저(P균＜0.01).결론 교고람조감가승고2형당뇨병병비주정성지방성간병대서혈장、간조직적류화경농도,정제량의뢰성,개선2형당뇨병병비주정성지방성간병대서혈당、혈지대사.
Objective To observe the influence ofgypenosides on hydrogen sulfide in liver tissue and plasma of rat with type 2 diabetes mellitus and nonalcoholic fatty liver disease.Methods 58 SPF male SD rats,with body mass 220～250 g,were randomly divided into a blank control group (group N,n=7),and a NAFLD and T2DM model group (Group M,n=51).Group N was fed with ordinary diet in the first four weeks,group M was fed with diets of high fat and sugar,injected with 40 mg/kg STZ overnight,and the same diets for the next four weeks.The rat model with T2DM and NAFLD was build.NAFLD and T2DM model group were divided into three groups:a high dose GPS group (JH,n=9) injected with 1 g/kg · d-1 GPS,a low dose GPS group (JL,n=9) injected with 0.5 g/kg · d-1 GPS,and a model group (M,n=9) injected with the same volume of water,and high fat diet at the same time.The treatment period was six weeks,and the experiment period was fourteen weeks.TG,TC,BS,and H2S in the plasma of rat were tested,and H2S in the liver tissue of the rat was tested.Results ①The changes of H2S in plasma:group JH [(4.30±0.43) μmol/L] and JL [(3.83 ±0.47) μmol/L] was lower than group M [(2.67 ± 0.41) μmol/L],there was a significant difference.②The changes of H2S in the liver tissue:group JH [(333.52±37.94) pmol/min/mg/protein] and JL [(275.81 ±36.07)pmol/min/mg/protein] was lower than group M [(237.8± 33.05) pmol/min/mg/protein],there was a significant difference.③BS levels:group JH(10.86±3.46)mmol/L,group JL (14.78±3.39)mmol/L,group M(18.84±4.24) mmol/L,group JH and JL was lower than group M,there was a significant difference (P＜0.01).④The plasma TG level:group N (0.96±0.09) mmol/L,group JH (2.82± 0.66) mmol/L,group JL (1.83± 0.56) mmol/L,group M (3.97 ± 0.64) mmol/L.group JH and JL was lower than group M,there was a significant difference (P＜0.01).Conclusion Gypenoside can reduce the blood sugar,triglycerides,and total cholesterol in rat with with type 2 diabetes mellitus and nonalcohol fatty liver disease.H2S concentrations in plasma and liver tissue of the rats with T2DM and NAFLD were increased by GPS,showing dose dependence.Gypenosides can also improve metabolism of blood glucose and lipid in rats with T2DM and NAFLD.