国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2013年
12期
1079-1081
,共3页
万凌峰%薛博瑜%柳璋璞%邵铭
萬凌峰%薛博瑜%柳璋璞%邵銘
만릉봉%설박유%류장박%소명
芪参清肝汤%肝癌%SMMC-7721细胞%凋亡
芪參清肝湯%肝癌%SMMC-7721細胞%凋亡
기삼청간탕%간암%SMMC-7721세포%조망
Qishen-Qinggan decoction%Hepatocellular carcinoma%SMMC-7721 cells%Apoptosis
目的 观察芪参清肝汤对人肝癌SMMC-7721细胞增殖和凋亡的影响.方法 制备芪参清肝汤,体外培养SMMC-7721细胞,取对数生长期细胞分为药物干预组及对照组,药物干预组分别加入0.135、0.27、0.54、1.08、2.16 g/ml芪参清肝汤处理12h、24 h、48 h,对照组不加药.采用四甲基偶氮唑兰(MTT)比色法检测2组细胞抑制率,采用流式细胞术检测2组细胞凋亡率.结果 0.135、0.27、0.54、1.08、2.16 g/m1芪参清肝汤对SMMC-7721细胞增殖有明显的抑制作用12h的OD值分别为0.89±0.05、0.85±0.05、0.80±0.06、0.78±0.02、0.69±0.07; 24 h的OD值分别为0.77±0.07、0.74±0.07、0.59±0.07、0.50±0.09、0.39±0.08; 48 h的OD值分别为0.78±0.05、0.61±0.08、0.44±0.10、0.39±0.08、0.34±0.07,并呈剂量、时间依赖性,与对照组(1.14±0.06、1.24±0.03、1.31±0.06)比较,差异均有统计学意义(P＜0.01); 0.27、0.54 g/ml芪参清肝汤能明显诱导SMMC-7721细胞凋亡(11.19±2.23、15.69±2.51),与对照组(1.41±0.22)比较有统计学意义(P＜0.05),1.08 g/ml芪参清肝汤诱导肝癌细胞凋亡(41.83±7.11),与对照组(1.41±0.22)比较,差异有统计学意义(P＜0.01).结论 芪参清肝汤能显著抑制人肝癌SMMC-7721细胞的增殖,可能通过诱导肝癌细胞凋亡发挥其作用.
目的 觀察芪參清肝湯對人肝癌SMMC-7721細胞增殖和凋亡的影響.方法 製備芪參清肝湯,體外培養SMMC-7721細胞,取對數生長期細胞分為藥物榦預組及對照組,藥物榦預組分彆加入0.135、0.27、0.54、1.08、2.16 g/ml芪參清肝湯處理12h、24 h、48 h,對照組不加藥.採用四甲基偶氮唑蘭(MTT)比色法檢測2組細胞抑製率,採用流式細胞術檢測2組細胞凋亡率.結果 0.135、0.27、0.54、1.08、2.16 g/m1芪參清肝湯對SMMC-7721細胞增殖有明顯的抑製作用12h的OD值分彆為0.89±0.05、0.85±0.05、0.80±0.06、0.78±0.02、0.69±0.07; 24 h的OD值分彆為0.77±0.07、0.74±0.07、0.59±0.07、0.50±0.09、0.39±0.08; 48 h的OD值分彆為0.78±0.05、0.61±0.08、0.44±0.10、0.39±0.08、0.34±0.07,併呈劑量、時間依賴性,與對照組(1.14±0.06、1.24±0.03、1.31±0.06)比較,差異均有統計學意義(P＜0.01); 0.27、0.54 g/ml芪參清肝湯能明顯誘導SMMC-7721細胞凋亡(11.19±2.23、15.69±2.51),與對照組(1.41±0.22)比較有統計學意義(P＜0.05),1.08 g/ml芪參清肝湯誘導肝癌細胞凋亡(41.83±7.11),與對照組(1.41±0.22)比較,差異有統計學意義(P＜0.01).結論 芪參清肝湯能顯著抑製人肝癌SMMC-7721細胞的增殖,可能通過誘導肝癌細胞凋亡髮揮其作用.
목적 관찰기삼청간탕대인간암SMMC-7721세포증식화조망적영향.방법 제비기삼청간탕,체외배양SMMC-7721세포,취대수생장기세포분위약물간예조급대조조,약물간예조분별가입0.135、0.27、0.54、1.08、2.16 g/ml기삼청간탕처리12h、24 h、48 h,대조조불가약.채용사갑기우담서란(MTT)비색법검측2조세포억제솔,채용류식세포술검측2조세포조망솔.결과 0.135、0.27、0.54、1.08、2.16 g/m1기삼청간탕대SMMC-7721세포증식유명현적억제작용12h적OD치분별위0.89±0.05、0.85±0.05、0.80±0.06、0.78±0.02、0.69±0.07; 24 h적OD치분별위0.77±0.07、0.74±0.07、0.59±0.07、0.50±0.09、0.39±0.08; 48 h적OD치분별위0.78±0.05、0.61±0.08、0.44±0.10、0.39±0.08、0.34±0.07,병정제량、시간의뢰성,여대조조(1.14±0.06、1.24±0.03、1.31±0.06)비교,차이균유통계학의의(P＜0.01); 0.27、0.54 g/ml기삼청간탕능명현유도SMMC-7721세포조망(11.19±2.23、15.69±2.51),여대조조(1.41±0.22)비교유통계학의의(P＜0.05),1.08 g/ml기삼청간탕유도간암세포조망(41.83±7.11),여대조조(1.41±0.22)비교,차이유통계학의의(P＜0.01).결론 기삼청간탕능현저억제인간암SMMC-7721세포적증식,가능통과유도간암세포조망발휘기작용.
Objective To investigate effect of Qishen-Qinggan decoction on proliferation and apoptosis of human hepatocellular carcinoma cellline SMMC-7721.Methods SMMC-7721 cells were cultivated in vitro.Logarithmic growth phase cells were divided into a drug intervention group and a control group.SMMC-7721 cells were treated with Qishen-Qinggan decoction of 0.135、0.27、0.54、1.08、2.16 g/ml respectively for 12、24、48 hours in the drug intervention group while the control group remained untreated.The inhibition rate of SMMC-7721 cells were detected by MTT assay,cell apoptotic rate were measured by flow cytometry analysis.Results Qishen-Qinggan decoction of 0.135,0.27,0.54,1.08,2.16 g/ml had a significantly inhibitory effect on SMMC-7721 cells in a dose and time dependent manner.OD values of 12 hours were 0.89±0.05,0.85±0.05,0.80±0.06,0.78± 0.02,0.69±0.07,OD values of 24 hours were 0.77±0.07,0.74±0.07,0.59±0.07,0.50±0.09,0.39±0.08,OD values of 48 hours were 0.78±0.05,0.61±0.08,0.44±0.10,0.39±0.08,0.34±0.07 respectively.Each drug intervention group had significant difference compared with control group.Qishen-Qinggan decoction of 0.27,0.54 g/ml could induce apoptosis of SMMC-7721 were 11.19 ± 2.23,15.69 ± 2.51,compared with control group of 1.41 ± 0.22.Apoptotic rates of Qishen-Qinggan decoction of 1.08 g/ml had extremely significant difference with the control group (41.83 ± 7.11 vs 1.41 ± 0.22).Conclusion Qishen-Qinggan decoction could inhibit the proliferation of SMMC-7721 cells probably by inducing cell apoptosis.
