CAJ | 학술논문

目的利用中药多成分吸收代谢评价方法,考察其对同植物不同药用部位多成分吸收及其吸收代谢差异的适用性.方法利用大鼠肠吸收代谢实验方法,对葛根素、葛根水提液、葛花水提液进行体外温孵代谢实验,并运用高效液相色谱法进行分析.结果葛根素单一成分代谢与葛根水提液、葛花水提液多成分共同代谢的对比结果显示,葛根水提液中葛根素代谢率低于葛根素对照品溶液,酶平均浓度可能是造成此差异的原因.此外,研究发现相同植物不同药用部位的葛根与葛花的化学成分指纹图谱存在很大差异,揭示二者所含成分差异较大.结论采用S9实验进行多成分肠吸收时的并发肠壁代谢研究,可揭示多成分的肠代谢总体特征.
목적 이용중약다성분흡수대사평개방법,고찰기대동식물불동약용부위다성분흡수급기흡수대사차이적괄용성.방법 이용대서장흡수대사실험방법,대갈근소、갈근수제액、갈화수제액진행체외온부대사실험,병운용고효액상색보법진행분석.결과 갈근소단일성분대사여갈근수제액、갈화수제액다성분공동대사적대비결과현시,갈근수제액중갈근소대사솔저우갈근소대조품용액,매평균농도가능시조성차차이적원인.차외,연구발현상동식물불동약용부위적갈근여갈화적화학성분지문도보존재흔대차이,게시이자소함성분차이교대.결론 채용S9실험진행다성분장흡수시적병발장벽대사연구,가게시다성분적장대사총체특정.
Objective To establish a method to compare the difference of exposure component of oral taking different part ofPueraria Lobata (Willd.) Ohwi in intestine.Methods After the incubation of puerarin,extraction Radix Puerariae and Flos Puerariae with S9,the mixtures were centrifuged to get the supernatant for analysis with HPLC.Results The research showed that the metabolic rate of puerarin was higher than that of extractions because of the concentration of enzyme.The difference between the fingerprints of Radix Puerariae and Flos Puerariae Lobata indicated that there was a difference of chemical components in such two parts of Kudzuvine Root.Conclusion The profile of intestinal metabolism can be revealed in the research of gut wall metabolism in the course of intestinal absorption by S9 incubation.