中华解剖与临床杂志
中華解剖與臨床雜誌
중화해부여림상잡지
Chinese Journal of Anatomy and Clinics
2014年
1期
58-61
,共4页
王丽华%张競%王亮亮%王蓓蓓
王麗華%張競%王亮亮%王蓓蓓
왕려화%장경%왕량량%왕배배
上皮性卵巢癌%Beclin 1%LC3-Ⅱ%P62%免疫组织化学
上皮性卵巢癌%Beclin 1%LC3-Ⅱ%P62%免疫組織化學
상피성란소암%Beclin 1%LC3-Ⅱ%P62%면역조직화학
Epithelia ovarian cancer%Beclin 1%Microtubule-associated protein 1 light chain3%P62%Immunohistochemistry
目的 探讨上皮性卵巢癌中Beclin 1、LC3-Ⅱ及P62蛋白的表达及临床意义.方法 选取2011年6月-2013年3月我院手术切除并经病理学检查确诊的上皮性卵巢癌标本50例为实验组,同期手术切除正常卵巢组织20例作为对照,通过免疫组织化学SP法,检测标本中Beclin 1、LC3-Ⅱ及P62蛋白的表达情况,并结合临床病理资料进行分析.结果 在上皮性卵巢癌组织中,Beclin 1、LC3-Ⅱ蛋白的阳性表达率分别为46.00％(23/50)和42.00％ (21/50),低于正常卵巢组织的95.00％(19/20)和85.00％(17/20);而P62蛋白的阳性表达率为72.00％(36/50),高于正常卵巢组织的10.00％(2/20),差异均具有统计学意义(P值均＜0.05).在上皮性卵巢癌组织中,Beclin 1、LC3-Ⅱ蛋白的表达与FIGO分期、病理分级具有相关性(P＜0.05),而P62蛋白的表达与FIGO分期具有相关性(P＜0.05).Beclin 1蛋白和LC3-Ⅱ蛋白在上皮性卵巢癌组织中的表达呈正相关(P＜0.01),P62蛋白的表达与Beclin 1、LC3-Ⅱ蛋白均呈负相关(P＜0.01).结论 在上皮性卵巢癌组织中,Beclin 1、LC3-Ⅱ和P62蛋白的异常表达,可导致自噬体形成减少和自噬功能减弱;可能与卵巢癌的发生、发展和预后不良有关.
目的 探討上皮性卵巢癌中Beclin 1、LC3-Ⅱ及P62蛋白的錶達及臨床意義.方法 選取2011年6月-2013年3月我院手術切除併經病理學檢查確診的上皮性卵巢癌標本50例為實驗組,同期手術切除正常卵巢組織20例作為對照,通過免疫組織化學SP法,檢測標本中Beclin 1、LC3-Ⅱ及P62蛋白的錶達情況,併結閤臨床病理資料進行分析.結果 在上皮性卵巢癌組織中,Beclin 1、LC3-Ⅱ蛋白的暘性錶達率分彆為46.00％(23/50)和42.00％ (21/50),低于正常卵巢組織的95.00％(19/20)和85.00％(17/20);而P62蛋白的暘性錶達率為72.00％(36/50),高于正常卵巢組織的10.00％(2/20),差異均具有統計學意義(P值均＜0.05).在上皮性卵巢癌組織中,Beclin 1、LC3-Ⅱ蛋白的錶達與FIGO分期、病理分級具有相關性(P＜0.05),而P62蛋白的錶達與FIGO分期具有相關性(P＜0.05).Beclin 1蛋白和LC3-Ⅱ蛋白在上皮性卵巢癌組織中的錶達呈正相關(P＜0.01),P62蛋白的錶達與Beclin 1、LC3-Ⅱ蛋白均呈負相關(P＜0.01).結論 在上皮性卵巢癌組織中,Beclin 1、LC3-Ⅱ和P62蛋白的異常錶達,可導緻自噬體形成減少和自噬功能減弱;可能與卵巢癌的髮生、髮展和預後不良有關.
목적 탐토상피성란소암중Beclin 1、LC3-Ⅱ급P62단백적표체급림상의의.방법 선취2011년6월-2013년3월아원수술절제병경병이학검사학진적상피성란소암표본50례위실험조,동기수술절제정상란소조직20례작위대조,통과면역조직화학SP법,검측표본중Beclin 1、LC3-Ⅱ급P62단백적표체정황,병결합림상병리자료진행분석.결과 재상피성란소암조직중,Beclin 1、LC3-Ⅱ단백적양성표체솔분별위46.00％(23/50)화42.00％ (21/50),저우정상란소조직적95.00％(19/20)화85.00％(17/20);이P62단백적양성표체솔위72.00％(36/50),고우정상란소조직적10.00％(2/20),차이균구유통계학의의(P치균＜0.05).재상피성란소암조직중,Beclin 1、LC3-Ⅱ단백적표체여FIGO분기、병리분급구유상관성(P＜0.05),이P62단백적표체여FIGO분기구유상관성(P＜0.05).Beclin 1단백화LC3-Ⅱ단백재상피성란소암조직중적표체정정상관(P＜0.01),P62단백적표체여Beclin 1、LC3-Ⅱ단백균정부상관(P＜0.01).결론 재상피성란소암조직중,Beclin 1、LC3-Ⅱ화P62단백적이상표체,가도치자서체형성감소화자서공능감약;가능여란소암적발생、발전화예후불량유관.
Objective To investigate the expression of autophagy-related gene Beclinl,microtubule-associated protein 1 light chain3 (LC3-Ⅱ) and P62 in ovarian cancer tissues and explore its clinical significance.Methods Fifty cases of epithelia ovarian cancer excision specimens definite by histopathology selected from our department from June 2011 to March 2013 were experimental group,and 20 cases of normal ovarian specimens as the control.Expressions of LC3-Ⅱ,Beclinl and P62 were detected by immunohistochemistry.The correlations of Beclinl,LC3-Ⅱ and P62 expression to the clinicopathologic characteristics of the 50 epithelial ovarian cancer patients were analyzed.Results The positive rates of LC3-Ⅱ and Beclinl were significantly lower in epithelial ovarian cancer than that in normal ovarian tissue (46.00％,23/50 vs 95.00％,19/20;42.00％,21/50 vs 85.00％,17/20),and the positive rates of P62 were significantly higher in epithelial ovarian cancer than that in normal ovarian tissue (72.00％,36/50 vs 10.00％,2/20 ; P ＜ 0.05).The expression of Beclinl and LC3-Ⅱ were associated with FIGO stage and histological grade (P ＜ 0.05).The expression of P62 was related to FIGO stage (P ＜ 0.05).Beclinl expression was positively correlated with the expression of LC3-Ⅱ (P ＜ 0.01).The expression of P62 was negatively correlated with Beclinl and LC3-Ⅱ expression (P ＜ 0.01).Conclusions The abnormal expression of Beclin 1,LC3-Ⅱ and P62 in epithelial ovarian carcinoma tissues lead to decrease of autophagosome formation and function of autophagy,and may be associated with ovarian cancer occurrence,development and prognosis.
