中华解剖与临床杂志
中華解剖與臨床雜誌
중화해부여림상잡지
Chinese Journal of Anatomy and Clinics
2014年
2期
154-158
,共5页
季涛%孔令尚%孙学童%张宗兵%王栓虎%汪华学%刘牧林
季濤%孔令尚%孫學童%張宗兵%王栓虎%汪華學%劉牧林
계도%공령상%손학동%장종병%왕전호%왕화학%류목림
缺血再灌注损伤%小肠%髓样细胞触发受体-1%肠黏膜屏障功能障碍%肿瘤坏死因子-α%小鼠
缺血再灌註損傷%小腸%髓樣細胞觸髮受體-1%腸黏膜屏障功能障礙%腫瘤壞死因子-α%小鼠
결혈재관주손상%소장%수양세포촉발수체-1%장점막병장공능장애%종류배사인자-α%소서
Ischemia reperfusion injury%Intestinal%Triggering recepter expressed on myeloid cell-1%Mucosal barrier dysfunction%Tumor necrosis factor-α%Mice
目的 建立小鼠肠缺血再灌注(I/R)损伤模型,观察小鼠小肠I/R损伤后髓样细胞触发受体-1(TREM-1)的表达及其与炎症因子水平变化的关系.方法 将72只小鼠按数字表法随机分为3组,对照组(N组)8只、假手术组(S组)32只、I/R损伤组(I/R组)32只.制备肠I/R损伤模型,制模后S组与I/R组分别于6、12、24、48 h处死8只小鼠取标本:ELISA测定外周血清中可溶性(s) TREM-1、TNF-α的水平并进行相关性分析;免疫组织化学检测小肠组织中TREM-1的表达.结果 I/R组24h时TREM-1浓度达峰值为(1 272.88±295.52) pg/ml,各时段浓度均高于N组[(168.99±22.79) pg/ml]和S组[24 h(178.58±10.98) pg/ml],差异均有统计学意义(P值均<0.01).I/R组TNF-α值12 h[(33.03 ±4.12) pg/ml]开始升高,24 h[(94.01±9.44) pg/ml]达峰值.sTREM-1表达和TNF-α浓度的变化呈正相关(r=0.840,P=0.000).免疫组化显示在I/R损伤后小鼠肠sTREM-1表达增高,24 h组染色积分最高为(3.38±0.66)分,且阳性部位主要是小肠黏膜固有层和上皮细胞.结论 小肠组织sTREM-1的表达上调可能是导致肠黏膜屏障功能下降及系统性炎症反应的重要环节;sTREM-1可作为判断肠I/R肠黏膜损伤严重程度的的检测指标.
目的 建立小鼠腸缺血再灌註(I/R)損傷模型,觀察小鼠小腸I/R損傷後髓樣細胞觸髮受體-1(TREM-1)的錶達及其與炎癥因子水平變化的關繫.方法 將72隻小鼠按數字錶法隨機分為3組,對照組(N組)8隻、假手術組(S組)32隻、I/R損傷組(I/R組)32隻.製備腸I/R損傷模型,製模後S組與I/R組分彆于6、12、24、48 h處死8隻小鼠取標本:ELISA測定外週血清中可溶性(s) TREM-1、TNF-α的水平併進行相關性分析;免疫組織化學檢測小腸組織中TREM-1的錶達.結果 I/R組24h時TREM-1濃度達峰值為(1 272.88±295.52) pg/ml,各時段濃度均高于N組[(168.99±22.79) pg/ml]和S組[24 h(178.58±10.98) pg/ml],差異均有統計學意義(P值均<0.01).I/R組TNF-α值12 h[(33.03 ±4.12) pg/ml]開始升高,24 h[(94.01±9.44) pg/ml]達峰值.sTREM-1錶達和TNF-α濃度的變化呈正相關(r=0.840,P=0.000).免疫組化顯示在I/R損傷後小鼠腸sTREM-1錶達增高,24 h組染色積分最高為(3.38±0.66)分,且暘性部位主要是小腸黏膜固有層和上皮細胞.結論 小腸組織sTREM-1的錶達上調可能是導緻腸黏膜屏障功能下降及繫統性炎癥反應的重要環節;sTREM-1可作為判斷腸I/R腸黏膜損傷嚴重程度的的檢測指標.
목적 건립소서장결혈재관주(I/R)손상모형,관찰소서소장I/R손상후수양세포촉발수체-1(TREM-1)적표체급기여염증인자수평변화적관계.방법 장72지소서안수자표법수궤분위3조,대조조(N조)8지、가수술조(S조)32지、I/R손상조(I/R조)32지.제비장I/R손상모형,제모후S조여I/R조분별우6、12、24、48 h처사8지소서취표본:ELISA측정외주혈청중가용성(s) TREM-1、TNF-α적수평병진행상관성분석;면역조직화학검측소장조직중TREM-1적표체.결과 I/R조24h시TREM-1농도체봉치위(1 272.88±295.52) pg/ml,각시단농도균고우N조[(168.99±22.79) pg/ml]화S조[24 h(178.58±10.98) pg/ml],차이균유통계학의의(P치균<0.01).I/R조TNF-α치12 h[(33.03 ±4.12) pg/ml]개시승고,24 h[(94.01±9.44) pg/ml]체봉치.sTREM-1표체화TNF-α농도적변화정정상관(r=0.840,P=0.000).면역조화현시재I/R손상후소서장sTREM-1표체증고,24 h조염색적분최고위(3.38±0.66)분,차양성부위주요시소장점막고유층화상피세포.결론 소장조직sTREM-1적표체상조가능시도치장점막병장공능하강급계통성염증반응적중요배절;sTREM-1가작위판단장I/R장점막손상엄중정도적적검측지표.
Objective To establish intestinal ischemia reperfusion injury model in mice and investigate the changes of triggering recepter expressed on myeloid cell-1 (TREM-1) expression in ileum after intestine ischemia reperfusion injury and the relationship between TREM-1 expression and TNF-α level in peripheral blood.Methods Seventy-two mice were randomly divided into 3 groups:normal control group (group N),sham operation group (group S),ischemia reperfusion injury group (group I/R).Intestinal ischemia reperfusion injury model was established,mice in group S and group I/R were respectively sacrificed after 6 h,12 h,24 h and 48 h,enzyme linked immunosorbent assay was used to detect the TREM-1 expression and TNF-α levels in peripheral blood and the correlation between TREM-1 expression and TNF-α levels was analyzed.The expression of TREM-1 in the small intestine tissue was observed by immunohistochemistry.Results The concentration of sTREM-1 in peripheral blood by ELISA in the group R/I reached the peak after 24 h and in this time point the concentration of sTREM-1 in peripheral blood in the group R/I was higher than the groups [(178.58 ± 10.98) pg/ml],the concentration in the group R/I displayed at all time points was higher compared with the group N [(168.99 ± 22.79) pg/ml].The difference was significant (all P values < 0.01).TNF-α level in the group R/I began to increase after 12 h [(33.03 ± 4.12) pg/ml] and reached the peak after 24 h [(94.01 ± 9.44) pg/ml],the changes of sTREM-1 expression were positively correlated with the TNF-α levels (r =0.840,P =0.000).Immunohistochemistry showed that the TREM-1 expression in the intestine in mice increased after ischemia reperfusion injury and the staining points was the highest in group I/R 24 h(3.38 ±0.66) and the positive parts were mainly small intestinal lamina propria and epithelial cells.Conclusions Upregulation of TREM-1 expression in intestinal tissue might lead to dysfunction of intestinal mucosal barrier and it might be an important link to the systemic inflammation.sTREM-1 could be used to determine the severity of intestinal mucosa injury in intestinal ischemia reperfusion injury.