CAJ | 학술논문

目的观察氢气对脓毒症小鼠肾损伤的保护作用,并初步探讨相关机制.方法采用盲肠结扎穿孔术诱导的小鼠脓毒症模型.24只雄性C57BL/6小鼠,体质量20～25 g,随机(随机数字法)分为4组:假手术组(Sham)、假手术+氢气吸入组(Sham+ H2)、脓毒症组(Sepsis)和脓毒症+氢气吸入组(Sepsis+ H2).氢气治疗为盲肠结扎穿孔术或假手术后1h和6h,分别吸入2％氢气1h.观察各组小鼠24 h时间点肾脏组织病理学变化和细胞凋亡情况,测定血肌酐(blood creatinine,Cr)和尿素氮(blood urea nitrogen,BUN)水平,同时检测血清及肾组织中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、人8异前列腺素F2α(8-iso-prostaglandin F2α,8-iso-PGF2α)和高迁移率族蛋白B1 (high mobility group box 1,HMGB1)的水平.结果吸入氢气可显著改善脓毒症小鼠肾组织损伤,减少肾组织细胞凋亡,提高血清及肾组织SOD和CAT活性,降低血清及肾组织8-iso-PGF2a和HMGB1水平(P＜0.05).结论吸入氢气对脓毒症小鼠急性肾损伤具有保护作用,其机制与提高机体抗氧化酶活性及降低炎症因子水平有关.
목적 관찰경기대농독증소서신손상적보호작용,병초보탐토상관궤제.방법 채용맹장결찰천공술유도적소서농독증모형.24지웅성C57BL/6소서,체질량20～25 g,수궤(수궤수자법)분위4조:가수술조(Sham)、가수술+경기흡입조(Sham+ H2)、농독증조(Sepsis)화농독증+경기흡입조(Sepsis+ H2).경기치료위맹장결찰천공술혹가수술후1h화6h,분별흡입2％경기1h.관찰각조소서24 h시간점신장조직병이학변화화세포조망정황,측정혈기항(blood creatinine,Cr)화뇨소담(blood urea nitrogen,BUN)수평,동시검측혈청급신조직중초양화물기화매(superoxide dismutase,SOD)、과양화경매(catalase,CAT)、인8이전렬선소F2α(8-iso-prostaglandin F2α,8-iso-PGF2α)화고천이솔족단백B1 (high mobility group box 1,HMGB1)적수평.결과 흡입경기가현저개선농독증소서신조직손상,감소신조직세포조망,제고혈청급신조직SOD화CAT활성,강저혈청급신조직8-iso-PGF2a화HMGB1수평(P＜0.05).결론 흡입경기대농독증소서급성신손상구유보호작용,기궤제여제고궤체항양화매활성급강저염증인자수평유관.
Objective To investigate the protective effects of hydrogen gas (H2) inhalation on acute renal injury in severe septic mice,and the associated mechanism.Methods Sepsis model was made by cecal ligation and puncture (CLP) operation in mice.A total of 24 mice were randomly (random number)divided into four groups:sham operation group (Sham),sham operation with H2 inhalation group (Sham +H2),sepsis group (Sepsis),and sepsis with H2 inhalation group (Sepsis + H2).The procedure for inhalation of 2％ H2 for 1 h was initiated at 1 h after CLP and 6 h after sham operation,respectively.The pathological changes and apoptosis of kidney,the blood creatinine (Cr) and blood urea nitrogen (BUN)concentration,the activities of superoxide dimutase (SOD) and catalase (CAT) in serum and kidney,as well as the levels of 8-iso-prostaglandin F2α (8-iso-PGF2α) and high mobility group box 1 (HMGB1) in serum and kidney were observed at 24 h after operation.Results H2 inhalation could significantly alleviate the renal injury of septic mice,reduce the renal cell apoptosis,increase the activities of SOD and CAT,as well as decrease the levels of 8-iso-PGF2α and HMGB1 in serum and kidney (P ＜ 0.05).Conclusions H2 inhalation can alleviate acute renal injury in septic mice,resulting from the increased activities of antioxidant enzymes and the reduced levels of oxidative products and inflammatory cytokines.