CAJ | 학술논문

目的探讨骨髓间充质干细胞(MSC)对重症急性胰腺炎(SAP)胰腺损伤的修复作用.方法将60只大鼠随机(随机数字法)分为健康对照组、SAP模型组、MSC移植组、MSC加粒细胞集落刺激因子(G-CSF)联合组.免疫荧光检测MSC的转化率,生化法检测血清淀粉酶含量,ELISA法检测血清白介素IL-6质量浓度.结果骨髓间充质干细胞转化率上升,血清淀粉酶含量和白介素IL-6质量浓度下降,增加粒细胞集落刺激因子(G-CSF)联合治疗,MSC细胞量较少时,产生协同作用增强MSC的修复作用,MSC细胞量较大时,产生拮抗作用加重胰腺组织的损伤.差异有统计学意义(P＜0.05).结论 MSC对SAP有明显修复损伤的作用,并随MCS细胞量增多,修复作用增强.增加粒细胞集落刺激因子(G-CSF)联合治疗,根据MSC细胞量的不同,产生协同作用增强MSC的修复作用,或者产生拮抗作用加重胰腺组织的损伤.
목적 탐토골수간충질간세포(MSC)대중증급성이선염(SAP)이선손상적수복작용.방법 장60지대서수궤(수궤수자법)분위건강대조조、SAP모형조、MSC이식조、MSC가립세포집락자격인자(G-CSF)연합조.면역형광검측MSC적전화솔,생화법검측혈청정분매함량,ELISA법검측혈청백개소IL-6질량농도.결과 골수간충질간세포전화솔상승,혈청정분매함량화백개소IL-6질량농도하강,증가립세포집락자격인자(G-CSF)연합치료,MSC세포량교소시,산생협동작용증강MSC적수복작용,MSC세포량교대시,산생길항작용가중이선조직적손상.차이유통계학의의(P＜0.05).결론 MSC대SAP유명현수복손상적작용,병수MCS세포량증다,수복작용증강.증가립세포집락자격인자(G-CSF)연합치료,근거MSC세포량적불동,산생협동작용증강MSC적수복작용,혹자산생길항작용가중이선조직적손상.
Objective To investigate effects of bone marrow mesenchymal stem cells (MSC) on severe acute pancreatitis (SAP) in rats.Methods A total of 60 rats were randomly divided into normal group (n =10),the model of SAP group (n =10),the transplantation of the MSC group (n =20),the combination of the MSC and the granulocyte colony-stimulating factor (G-CSF) group (n =20).The conversion rate of MSC was detected by using immunofluorescence methods,and the level of amylase in serum was assayed by using biochemical methods.Simultaneously,the level of interleukin-6 (IL-6) in serum was determined by using enzyme linked immunosorbent assay (ELISA).Results The conversion rates of the MSC increased,and consequently,the levels of amylases and interleukin-6 in serum were reduced (P ＜ 0.05).When a small amount of the G-CSF was added to MSC,the therapeutic effects of the two different kinds of cells were synergistically strengthened.In the contrary,when a large number of the G-CSF was added to MSC,the antagonism resulted between these two different kinds of cells gives rise to harmful effects on SAP.Conclusions The bone marrow mesenchymal stem cells have therapeutic effects on SAP.When the number of the bone marrow mesenchymal stem cells increases,the protective effects are enhanced.