CAJ | 학술논문

目的观察代谢性抗氧化剂硫辛酸对急性百草枯中毒造成的急性肺损伤/急性呼吸窘迫综合征肺组织中肺泡表面活性物质(SP-A/B)浓度的影响,及其影响是否与肿瘤坏死因子(TNF-α)有关.探讨硫辛酸对急性肺损伤的可能保护机制.方法购买华中科技大学同济医学院实验动物中心健康雄性Sprage-Dawley大鼠66只,随机(随机数字法)分3组:正常对照组(NS)6只、百草枯中毒组(PQ) 30只、百草枯+硫辛酸治疗组(LA) 30只.PQ组和LA组再细分5个亚组,即3、6、12、24、48 h亚组,每个亚组6只.在华中科技大学同济医学院附属同济医院胸外科实验中心实验,取大鼠相同部位的肺组织行HE染色,光镜下观察组织学变化,病理特点表现炎症反应和间质纤维化为模型成功;取另外相同部位肺组织检测超氧化物歧化酶(SOD)、丙二醛(MDA)水平,取大鼠尾静脉非抗凝全血0.8 mL测定TNF-α水平;提取肺组织中总RNA,用RT-PCR法测SP-A、SP-B mRNA水平.结果 ①HE染色结果显示LA组肺组织损伤较PQ组轻.②MDA和SOD数据得出:各时间点三组之间,组内各时间点之间比较,除3h时点组间外差异均具有统计学意义(P＜0.01).③TNF-α水平的数据:各时间点三组之间、组内各时间点之间比较,差异均具有统计学意义(P＜0.01).④SP-AmRNA和SP-BmRNA扩增倍数:各时间点的三组之间比较,差异具有统计学意义(P＜0.01);PQ组内各时间点之间比较,差异具有统计学意义(P＜0.05);LA组内各时间点之间比较,差异具有统计学意义(P＜0.01).结论硫辛酸在急性百草枯中毒中能使肺组织的损伤减轻,其机制可能是通过直接调控TNF-α的水平,达到间接影响肺表面活性物质含量,从而减轻肺水肿,改善肺顺应性,保护肺脏组织.
목적 관찰대사성항양화제류신산대급성백초고중독조성적급성폐손상/급성호흡군박종합정폐조직중폐포표면활성물질(SP-A/B)농도적영향,급기영향시부여종류배사인자(TNF-α)유관.탐토류신산대급성폐손상적가능보호궤제.방법 구매화중과기대학동제의학원실험동물중심건강웅성Sprage-Dawley대서66지,수궤(수궤수자법)분3조:정상대조조(NS)6지、백초고중독조(PQ) 30지、백초고+류신산치료조(LA) 30지.PQ조화LA조재세분5개아조,즉3、6、12、24、48 h아조,매개아조6지.재화중과기대학동제의학원부속동제의원흉외과실험중심실험,취대서상동부위적폐조직행HE염색,광경하관찰조직학변화,병리특점표현염증반응화간질섬유화위모형성공;취령외상동부위폐조직검측초양화물기화매(SOD)、병이철(MDA)수평,취대서미정맥비항응전혈0.8 mL측정TNF-α수평;제취폐조직중총RNA,용RT-PCR법측SP-A、SP-B mRNA수평.결과 ①HE염색결과현시LA조폐조직손상교PQ조경.②MDA화SOD수거득출:각시간점삼조지간,조내각시간점지간비교,제3h시점조간외차이균구유통계학의의(P＜0.01).③TNF-α수평적수거:각시간점삼조지간、조내각시간점지간비교,차이균구유통계학의의(P＜0.01).④SP-AmRNA화SP-BmRNA확증배수:각시간점적삼조지간비교,차이구유통계학의의(P＜0.01);PQ조내각시간점지간비교,차이구유통계학의의(P＜0.05);LA조내각시간점지간비교,차이구유통계학의의(P＜0.01).결론 류신산재급성백초고중독중능사폐조직적손상감경,기궤제가능시통과직접조공TNF-α적수평,체도간접영향폐표면활성물질함량,종이감경폐수종,개선폐순응성,보호폐장조직.
Objective To observe the changes in concentrations of pulmonary surfactant SP-A/B in lung tissue during acute lung injury (ALI) /acute respiratory distress syndrome (ARDS) induced by acute paraquat poisoning (PQP) after the treatment with metabolic antioxidant,lipoic acid,and to explore the potential involvement of TNF-α in ALI/ARDS as well as to discuss the assumed protective mechanisms of lipoic acid against acute lung injury.Methods Sixty-six male Sprage-Dawley rats were randomly (random number) divided into 3 groups,namely control group (NS,n =6),paraquat poisoning group (PQ,n =30),paraquat + lipoic acid treatment group (LA,n =30).Then both group PQ and group LA were further divided separately into five subgroups,namely 3,6,12,24 and 48 h subgroups (n =6 in each subgroup).After rats sacrificed,the lung tissues were selected,and after HE staining,histological changes were observed under light microscope.Histopathological changes were inflammation and fibrosis in models successfully established.The lung tissues were also taken for tests of SOD and MDA levels.Specimens of whole blood 0.8 mL without anticoagulant were taken from tail vein of rats for determining the TNF-α level.The expressions of SP-A mRNA and SP-B mRNA were measured with RT-PCR from total RNA of the lung tissue.Results ① HE staining showed that the histopathological changes were milder in LA group than that in PQ group.② There were significant differences in MDA and SOD levels between different intervals both in intergroups and intragroup except the groups of 3 hours (P ＜ 0.01).③ Likewise,the significant differences in the levels of TNF-α were also present between three groups and between different intervals (P＜0.01).④ The significant differences in SP-A mRNA and SP-B mRNA amplification ratio existed between three groups at the same interval (P ＜ 0.01),but those differences between different intervals in group PQ were of statistical significance (P ＜ 0.05).And those differences between diffirent intervals in group LA were statistically significant (P ＜0.01).Conclusions Lipoic acid in acute paraquat poisoning could lessen lung tissue damage,which might be directly dominated by the levels of tumor necrosis factor,and in turn indirectly affect the content of pulmonary surfactant,thereby reducing pulmonary edema and improving lung compliance,then protecting the lung tissues.