中华急诊医学杂志
中華急診醫學雜誌
중화급진의학잡지
CHINESE JOURNAL OF EMERGENCY MEDICINE
2014年
1期
56-60
,共5页
许益聘%林和敏%谭健%韩芳%刘广鹏%叶信海
許益聘%林和敏%譚健%韓芳%劉廣鵬%葉信海
허익빙%림화민%담건%한방%류엄붕%협신해
脂肪干细胞%骨质疏松%卵巢切除术%兔%藻酸钙凝胶
脂肪榦細胞%骨質疏鬆%卵巢切除術%兔%藻痠鈣凝膠
지방간세포%골질소송%란소절제술%토%조산개응효
Adipose-derived stem cell%Osteoporosis%Ovariectomy%Rabbit%Calcium alginate hydrogel
目的 观察自体脂肪干细胞(adipose-derived stem cells,ASCs)移植对双侧卵巢切除术后骨质疏松(ovariectomy osteoporosis,OVX-OP)兔的影响.方法 15只成年新西兰雌性大白兔随机分为OVX模型组(A组,n=12)与假手术组(B组,n=3).A组行双侧卵巢切除术,B组行假手术.术后6个月,A、B组行双能量X线吸收测定(dual energy X-ray absorptiometry,DXA)检测骨密度(bone mineral density,BMD),验证造模效果.取OP兔皮下脂肪,体外培养并成骨诱导ASCs,行细胞计数,碱性磷酸酶(alkaline phosphatase,ALP)、茜素红染色及骨钙素(osteocalcin,OCN)等检测.取第3代成骨诱导ASCs复合藻酸钙凝胶(calcium alginate hydrogel,CAH),于OP模型兔左侧股骨远端注射ASCs-CAH,而右侧股骨注射单纯CAH材料作为治疗对照组.移植12周后作骨密度测定、Micro-CT检测及骨组织形态学分析.结果 与B组相比,A组股骨骨密度明显降低(P<0.05),证实OVX造模成功;体外成骨诱导培养的ASCs细胞计数与未诱导组相比,差异无统计学意义(P>0.05),而ALP、OCN检测及茜素红染色证实ASCs已分化为成骨细胞;自体细胞移植治疗12周后,与对照组相比,细胞治疗组股骨远端骨密度、骨小梁数量、厚度、分离度及结构模型指数均明显改善(P<0.05).组织学检测发现实验组骨小梁厚度增宽,植入的CAH材料已降解完全,而对照组骨小梁较纤细,CAH材料有部分残留.结论 ASCs自体移植对兔OP有一定的治疗效果,为OP治疗提供新思路.
目的 觀察自體脂肪榦細胞(adipose-derived stem cells,ASCs)移植對雙側卵巢切除術後骨質疏鬆(ovariectomy osteoporosis,OVX-OP)兔的影響.方法 15隻成年新西蘭雌性大白兔隨機分為OVX模型組(A組,n=12)與假手術組(B組,n=3).A組行雙側卵巢切除術,B組行假手術.術後6箇月,A、B組行雙能量X線吸收測定(dual energy X-ray absorptiometry,DXA)檢測骨密度(bone mineral density,BMD),驗證造模效果.取OP兔皮下脂肪,體外培養併成骨誘導ASCs,行細胞計數,堿性燐痠酶(alkaline phosphatase,ALP)、茜素紅染色及骨鈣素(osteocalcin,OCN)等檢測.取第3代成骨誘導ASCs複閤藻痠鈣凝膠(calcium alginate hydrogel,CAH),于OP模型兔左側股骨遠耑註射ASCs-CAH,而右側股骨註射單純CAH材料作為治療對照組.移植12週後作骨密度測定、Micro-CT檢測及骨組織形態學分析.結果 與B組相比,A組股骨骨密度明顯降低(P<0.05),證實OVX造模成功;體外成骨誘導培養的ASCs細胞計數與未誘導組相比,差異無統計學意義(P>0.05),而ALP、OCN檢測及茜素紅染色證實ASCs已分化為成骨細胞;自體細胞移植治療12週後,與對照組相比,細胞治療組股骨遠耑骨密度、骨小樑數量、厚度、分離度及結構模型指數均明顯改善(P<0.05).組織學檢測髮現實驗組骨小樑厚度增寬,植入的CAH材料已降解完全,而對照組骨小樑較纖細,CAH材料有部分殘留.結論 ASCs自體移植對兔OP有一定的治療效果,為OP治療提供新思路.
목적 관찰자체지방간세포(adipose-derived stem cells,ASCs)이식대쌍측란소절제술후골질소송(ovariectomy osteoporosis,OVX-OP)토적영향.방법 15지성년신서란자성대백토수궤분위OVX모형조(A조,n=12)여가수술조(B조,n=3).A조행쌍측란소절제술,B조행가수술.술후6개월,A、B조행쌍능량X선흡수측정(dual energy X-ray absorptiometry,DXA)검측골밀도(bone mineral density,BMD),험증조모효과.취OP토피하지방,체외배양병성골유도ASCs,행세포계수,감성린산매(alkaline phosphatase,ALP)、천소홍염색급골개소(osteocalcin,OCN)등검측.취제3대성골유도ASCs복합조산개응효(calcium alginate hydrogel,CAH),우OP모형토좌측고골원단주사ASCs-CAH,이우측고골주사단순CAH재료작위치료대조조.이식12주후작골밀도측정、Micro-CT검측급골조직형태학분석.결과 여B조상비,A조고골골밀도명현강저(P<0.05),증실OVX조모성공;체외성골유도배양적ASCs세포계수여미유도조상비,차이무통계학의의(P>0.05),이ALP、OCN검측급천소홍염색증실ASCs이분화위성골세포;자체세포이식치료12주후,여대조조상비,세포치료조고골원단골밀도、골소량수량、후도、분리도급결구모형지수균명현개선(P<0.05).조직학검측발현실험조골소량후도증관,식입적CAH재료이강해완전,이대조조골소량교섬세,CAH재료유부분잔류.결론 ASCs자체이식대토OP유일정적치료효과,위OP치료제공신사로.
Objective To observe the effects of transplantation of autologous adipose-derived stem cells (ASCs) on osteoporosis (OP) in a rabbit ovariectomy (OVX) model.Methods A total of fifteen 6-month-old female New Zealand white rabbits were randomly divided into two groups:ovariectomy group (group A,n =12) and sham operation group (group B,n =3).All rabbits were subjected to bilateral ovariectomy in the group A.Six months later,bone mineral density (BMD) of group A and group B were measured by dual energy X-ray absorptiometry (DXA) to check the result of OVX-OP.ASCs harvested from adipose of OP rabbits were cultured to be expanded and differentiated in osteogenic medium in vitro.Osteogenesis was evaluated by alizarin red staining,alkaline phosphatase (ALP) staining and quantitative assays of osteocalcin (OCN).Autologous osteo-induced ASCs were mixed in calcium alginate hydrogel (CAH) and then transplanted in the left distal femurs,while CAH was transplanted in the right distal femurs of OP rabbits.At 12 weeks after implantation,BMD,micro-CT and histomorphological analyses were performed on these rabbits.Results The BMD of femurs in group A rabbits were obviously lower than that of group B rabbits (P < 0.05) at 6 months after OVX.Compared with control group,ASCs cultured in osteo-induction medium had similar proliferation rate as the non-induced cells,but displayed positive ALP and alizarin red staining and OCN contents.At 12 weeks after implantation,the cell-treated femurs displayed higher BMD,bone trabecula number,trabecula thickness and separation than those of control group,while the structure model index and porosity were lower (P < 0.05).Histological examination indicated that the trabecular thickness increased with complete CAH resorption in cell-treated group,while CAH remained in control group.Conclusions Transplantation of autologous ASCs can help strengthen osteoporotic bone in OVX-OP rabbits,providing a novel approach to OP treatment.