CAJ | 학술논문

目的研究大剂量山莨菪碱联合乌司他丁对急性胰腺炎大鼠的治疗效果.方法健康成年SD大鼠100只,实验前12 h禁食,自由饮水,随机(随机数字法)分为5组:健康对照组、SAP组、SAP+乌司他丁治疗对照组、SAP+山莨菪碱治疗组、SAP乌司他丁加大剂量山莨菪碱治疗组各20只.观察各组大鼠的淀粉酶、磷脂酶A2、内毒素检测结果和组织学变化.另外再取SD大鼠60只,随机分为3组:SAP组、SAP+乌司他丁治疗对照组、SAP+乌司他丁及大剂量山莨菪碱治疗组,制作方法同前,观察生存情况.结果 SAP的血淀粉酶、PLA2、内毒素水平比三个治疗组明显升高(P＜0.05),同时组织学破坏最严重；而其他三个治疗组的各项指标明显降低,单独应用山莨菪碱治疗SAP也有一定效果,其中以SAP+ UTI+ 654-2组最明显,病理学改变也最轻,生存时间也是最长.结论山莨菪碱能够有效松弛Oddi括约肌,乌司他丁属蛋白酶抑制剂,对多种酶有抑制作用,联合应用可抑制SAP的炎性介质过度表达,对治疗SAP具有创新意义.
목적 연구대제량산랑탕감연합오사타정대급성이선염대서적치료효과.방법 건강성년SD대서100지,실험전12 h금식,자유음수,수궤(수궤수자법)분위5조:건강대조조、SAP조、SAP+오사타정치료대조조、SAP+산랑탕감치료조、SAP오사타정가대제량산랑탕감치료조각20지.관찰각조대서적정분매、린지매A2、내독소검측결과화조직학변화.령외재취SD대서60지,수궤분위3조:SAP조、SAP+오사타정치료대조조、SAP+오사타정급대제량산랑탕감치료조,제작방법동전,관찰생존정황.결과 SAP적혈정분매、PLA2、내독소수평비삼개치료조명현승고(P＜0.05),동시조직학파배최엄중；이기타삼개치료조적각항지표명현강저,단독응용산랑탕감치료SAP야유일정효과,기중이SAP+ UTI+ 654-2조최명현,병이학개변야최경,생존시간야시최장.결론 산랑탕감능구유효송이Oddi괄약기,오사타정속단백매억제제,대다충매유억제작용,연합응용가억제SAP적염성개질과도표체,대치료SAP구유창신의의.
Objective To study the effects of large dose anisodamine (654-2) combined with ulinastain on severe acute pancreatitis (SAP).Methods 100 healthy adult SD rats which were fasted 12 hours before experiment and were allowed drinking water freely,were divided to 5 groups randomly (random number):normal control group,SAPgroup,SAP + Ulinastain group,SAP + Anisodamine group,SAP +Ulinastain + Anisodamine,there were 20 rats in every group.To observe the levels of diastase,phospholipase A2 (PLA2) and endotoxin and pathology of rats in every group.Another cohort of 60 SD rats were divided into 3 groups:SAP group,SAP + Ulinastain group,SAP + Ulinastain + large dose Anisodamine group,survival periods were observed.Results The levels of diastase,PLA2 and endotoxin in SAP rats were higher than those in 3 SAP with treatment groups (P ＜ 0.05).The histopathological changes were most severe in SAP group.All of 3 markers in 3 SAP with treatment groups decreased obviously,and anisodamine alone was effective to treat SAP,but the effect of UTI + 654-2 was better than UTI or 654-2 alone,and histopathological changes were mild in this group treated with UTI + 654-2.Conclusions Anisodamine could effectively relax the Oddi sphincter thereby decreasing the hydrostatic pressure inside the bile duct and pancreatic duct.Ulinastain is a kind of proteinase inhibitor suppressing many kinds of enzymes and in tern to stabilize lysosomal membrane and inhibit the release of lysosomal enzyme.Combination of the large dose Anisodamine with Ulinastain could inhibit the overexpression of inflammationarv factors in SAP,thereby lessening the severity of viscera injury.