CAJ | 학술논문

目的研究旨在观察性别差异对经食道心室起搏致心脏骤停小鼠心肺复苏疗效是否有影响.方法 30只昆明小鼠,雌雄各半(F-gro=15,M-gro=15),经食道快速起搏心室诱发室颤、建立心脏骤停模型,起搏开始后4 min经动脉注射肾上腺素0.02 mg/kg,立即开始胸外心脏按压及机械通气,观察自主循环恢复情况,10 min无效则放弃复苏.自主循环恢复的小鼠连续监测心电和血压60 min,观察血压、心率、呼吸恢复情况及生存时间.结果雌性小鼠15只全部恢复自主循环,雄性小鼠15有14只恢复自主循环,两组小鼠的自主循环恢复时间、呼吸出现时间以及生存时间差异均无统计学意义[(50±17)s vs.(46±12)s;(2.4± 1)min vs.(2.5±1)min;28(1,72)h vs.16(3,72)h,P＞0.05)],且两组小鼠在恢复自主循环后lh内血压和心率均无显著性差异.结论与报道不同,性别差异并未影响昆明小鼠的心肺复苏疗效,其机制尚不清楚,还有待进一步研究.
목적 연구지재관찰성별차이대경식도심실기박치심장취정소서심폐복소료효시부유영향.방법 30지곤명소서,자웅각반(F-gro=15,M-gro=15),경식도쾌속기박심실유발실전、건립심장취정모형,기박개시후4 min경동맥주사신상선소0.02 mg/kg,립즉개시흉외심장안압급궤계통기,관찰자주순배회복정황,10 min무효칙방기복소.자주순배회복적소서련속감측심전화혈압60 min,관찰혈압、심솔、호흡회복정황급생존시간.결과 자성소서15지전부회복자주순배,웅성소서15유14지회복자주순배,량조소서적자주순배회복시간、호흡출현시간이급생존시간차이균무통계학의의[(50±17)s vs.(46±12)s;(2.4± 1)min vs.(2.5±1)min;28(1,72)h vs.16(3,72)h,P＞0.05)],차량조소서재회복자주순배후lh내혈압화심솔균무현저성차이.결론 여보도불동,성별차이병미영향곤명소서적심폐복소료효,기궤제상불청초,환유대진일보연구.
Objective Objectives To investigate the gender difference affecting the efficacy of cardiopulmonary resuscitation (CPR) in the mouse cardiac arrest (CA) model.Methods CA was induced in 30 Kunming mice (15 male and 15 female) by trans-oesophageal cardiac pacing for 4 minutes.Epinephrine was then administrated intra-artery,and CPR was performed.The time required for restoration of spontaneous circulation (ROSC) was observed,but if ROSC failed to appear at 10 minutes after CPR,resuscitation was discontinued.Blood pressure and electrocardiograms of resuscitated animals were invasively monitored for an additional 60 minutes.Blood pressure,heart rate,the restoration of spontaneous respiration (ROSR) and survival time were observed and recorded.Results All 15 female mice and 14 of 15 male mice had ROSC.There were no significant differences in the time required for ROSC,ROSR,and survival between the two groups [(50±17)svs.(46±12)s; (2.4±1)minvs.(2.5±1)min; 28 (1,72)h vs.16 (3,72)h,P ＞ 0.05)].Moreover,neither blood pressure nor heart rate showed significant differences one hour after ROSC between the two groups.Conclusions Sex differences did not affect the efficacy of CPR,but the precise mechanism is still unclear,and further investigations are required.