CAJ | 학술논문

目的探索可重复的严重大鼠挤压综合征模型.方法武汉大学中南医院动物实验中心,清洁级雄性SD大鼠50只,随机(随机数字法)分为5组,麻醉后用3.5 kg的重物按双侧后肢特殊槽式(SP组)、双侧后肢常规压迫(NM组)及单纯对照不压迫(SHAM组)6h,再灌注3h(每组10只大鼠).所有大鼠在压迫开始前和再灌注3h后测量动脉压、血乳酸、K+,CK,BUN,CRN及MB,相应的局部肌肉及肾脏进行病理切片检查.另外,进行相同的试验方法,监测观察双侧后肢常规压迫(D组)、双侧后肢特殊槽式(E组)生存率72 h.采用SPSS 17.0统计软件进行统计分析,重复测量数据的方差分析分析多组之间差异,Kaplan-Meier方法分析生存率.结果双侧后肢槽式组压迫较常规压迫组和单纯对照组增高了血乳酸(F=39.626,P＜0.05),AST(F=24.965,P＜0.05),ALT (F=19.096,P＜0.05),BUN (F=7.938,P＜0.05),CR (F=14.787,P＜0.05)及MB (F=16.840,P＜0.05)的水平,组织学标本中发现细胞损伤和缺血-再灌注损伤,双侧后肢卡槽式压迫在24h后导致潜在的急性肾小管坏死,常规式压迫组大鼠病死率20％,双侧后肢槽式挤压的病死率可达90％ (P ＜0.05).结论该模型是一个有效的大鼠严重挤压综合征模型,有应用价值.
목적 탐색가중복적엄중대서제압종합정모형.방법 무한대학중남의원동물실험중심,청길급웅성SD대서50지,수궤(수궤수자법)분위5조,마취후용3.5 kg적중물안쌍측후지특수조식(SP조)、쌍측후지상규압박(NM조)급단순대조불압박(SHAM조)6h,재관주3h(매조10지대서).소유대서재압박개시전화재관주3h후측량동맥압、혈유산、K+,CK,BUN,CRN급MB,상응적국부기육급신장진행병리절편검사.령외,진행상동적시험방법,감측관찰쌍측후지상규압박(D조)、쌍측후지특수조식(E조)생존솔72 h.채용SPSS 17.0통계연건진행통계분석,중복측량수거적방차분석분석다조지간차이,Kaplan-Meier방법분석생존솔.결과 쌍측후지조식조압박교상규압박조화단순대조조증고료혈유산(F=39.626,P＜0.05),AST(F=24.965,P＜0.05),ALT (F=19.096,P＜0.05),BUN (F=7.938,P＜0.05),CR (F=14.787,P＜0.05)급MB (F=16.840,P＜0.05)적수평,조직학표본중발현세포손상화결혈-재관주손상,쌍측후지잡조식압박재24h후도치잠재적급성신소관배사,상규식압박조대서병사솔20％,쌍측후지조식제압적병사솔가체90％ (P ＜0.05).결론 해모형시일개유효적대서엄중제압종합정모형,유응용개치.
Objective To investigate a reproducible model of severe crush injury (CI) in rats.Methods A total of 50 clean grade male SD rats were randomly (random number) divided into 5 groups.Both hindlimbs of anesthetized rats were compressed by blocks weighing 3.5 kg,for 6 hours and followed by 3 hours of reperfusion on a specially notched device (group SP,n =10),ordinary compression (group NM,n =10) and simple control (group SHAM,n =10).Arterial tension,serum lactate,and potassium (K+),serum myoglobin (MB),aspartate transferase (AST) and alanine transferase (ALT),BUN and Cr were measured at 10 minutes after cannulaton,and 3 hours after release from compression.Muscles and kidneys were evaluated morphologically.Group D and E were treated in the same way and were observed for 72 h to get the survival rate of the NM group and the specially notched compression group.The SPSS 17.0 statistical software was used for statistical analysis,repeated-measures ANOVA analysis for the differences between groups,Kaplan Meier-estimator for survival analysis.Results The Specially notched compression produced a greater increase in serum lactate (F =39.626,P ＜ 0.05),AST (F =24.965,P ＜ 0.05),ALT (F =19.096,P＜0.05),BUN (F=7.938,P＜0.05),CR (F=14.787,P＜0.05) and MB (F=16.840,P ＜0.05) by the end of experiment than NM group and simple control group.The direct cellular damage and ischemia-reperfusion injury were found under microscope.In crush injury caused by specially notched compression there was acute tubular necrosis found at 24 hours after injury.Mortality rate in the NM group was 20％,whereas mortality rate reached 90％ in rats with specially notched compression (P ＜0.05).Conclusions It successfully developed a severe crush injury model in experimental rats,suggesting it is worthwhile to popularization.