CAJ | 학술논문

目的探讨强化剂量阿托伐他汀对不稳定心绞痛患者PCI围术期CD4+T淋巴细胞SOCS1表达的影响.方法入选广西医科大学第一附属医院住院的不稳定型心绞痛患者50例,用随机数字法分为强化他汀组(术前80 mg/d,术后40 mg/d,n=25)和常规他汀组(术前术后均20 mg/d,n=25).分别于PCI术前、术后18～24 h抽取新鲜外周血,免疫磁珠法分选出CD4+T淋巴细胞,荧光定量PCR检测SOCS1 mRNA表达,蛋白免疫印迹法检测SOCS1蛋白表达,酶联免疫法检测IFN-γ炎症因子的表达.结果 ①与PCI术前强化组相比,术后强化组SOCS1 mRNA、SOCS1蛋白表达明显升高(P＜0.05).②与PCI术前常规组相比,术后常规组SOCS1 mRNA、SOCS1蛋白表达有升高的趋势,但差异无统计学意义(P＞0.05)③与PCI术前常规组比较,术后常规组血清IFN-γ浓度明显升高(P＜0.05);与PCI术前强化组比较,术后强化组血清IFN-γ浓度有升高的趋势,但差异无统计学意义(P＞0.05).结论强化剂量的阿托伐他汀可以通过上调CD4+T淋巴细胞SOCS1的表达,从而减少PCI术后心肌的炎症反应.
목적 탐토강화제량아탁벌타정대불은정심교통환자PCI위술기CD4+T림파세포SOCS1표체적영향.방법 입선엄서의과대학제일부속의원주원적불은정형심교통환자50례,용수궤수자법분위강화타정조(술전80 mg/d,술후40 mg/d,n=25)화상규타정조(술전술후균20 mg/d,n=25).분별우PCI술전、술후18～24 h추취신선외주혈,면역자주법분선출CD4+T림파세포,형광정량PCR검측SOCS1 mRNA표체,단백면역인적법검측SOCS1단백표체,매련면역법검측IFN-γ염증인자적표체.결과 ①여PCI술전강화조상비,술후강화조SOCS1 mRNA、SOCS1단백표체명현승고(P＜0.05).②여PCI술전상규조상비,술후상규조SOCS1 mRNA、SOCS1단백표체유승고적추세,단차이무통계학의의(P＞0.05)③여PCI술전상규조비교,술후상규조혈청IFN-γ농도명현승고(P＜0.05);여PCI술전강화조비교,술후강화조혈청IFN-γ농도유승고적추세,단차이무통계학의의(P＞0.05).결론 강화제량적아탁벌타정가이통과상조CD4+T림파세포SOCS1적표체,종이감소PCI술후심기적염증반응.
Objective To investigate the effects of aggressive dosing of atorvastatin on the expression of SOCS1 in CD4 + Tlymphocytes from patients with unstable angina pectoris during peri-operative period of PCI.Methods A cohort of 50 patients with unstable angina pectoris were randomized (random number) to give pretreatment with either an aggressive dose (80 mg/d,n =25) or a routine dose (20 mg/d,n =25)of atorvastatin.Circulating CD4 +T cells were subsequently obtained prior to PCI,and also 18 h to 24 hours after PCI,using a magnetic cell sorting system (MACS).Fluorescence-based quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure expressions of SOCSI mRNA in the isolated CD4 + Tlymphocytes,and western blot analysis was used to detect levels of SOCS1 protein.Serum levels of IFN-γwere quantified using enzyme-linked immunosorbent assays (ELISAs).Results Compared with routine dose group,the expressions of SOCS1 mRNA and protein levels were dramatically increased and those were higher in aggressive dose group following PCI (P ＜ 0.05).In contrast,serum levels of IFN-γsignificantly increased following PCI in both groups,but it was higher in routine dose group than in aggressive dose group (P ＜ 0.05).Conclusions Treatment with aggressive dosing of atorvastatin reduced the post-PCI myocardial inflammatory response in patients with unstable angina pectoris,possibly modulating by up-regulating SOCS1 expression in CD4 + Tlymphocytes.